A chromosome-scale genome assembly of a diploid alfalfa, the progenitor of autotetraploid alfalfa.

Alfalfa (Medicago sativa L.) is one of the most important and widely cultivated forage crops. It is commonly used as a vegetable and medicinal herb because of its excellent nutritional quality and significant economic value. Based on Illumina, Nanopore and Hi-C data, we assembled a chromosome-scale assembly of Medicago sativa spp. caerulea (voucher PI464715), the direct diploid progenitor of autotetraploid alfalfa. The assembled genome comprises 793.2 Mb of genomic sequence and 47,202 annotated protein-coding genes. The contig N50 length is 3.86 Mb. This genome is almost twofold larger and contains more annotated protein-coding genes than that of its close relative, Medicago truncatula (420 Mb and 44,623 genes). The more expanded gene families compared with those in M. truncatula and the expansion of repetitive elements rather than whole-genome duplication (i.e., the two species share the ancestral Papilionoideae whole-genome duplication event) may have contributed to the large genome size of M. sativa spp. caerulea. Comparative and evolutionary analyses revealed that M. sativa spp. caerulea diverged from M. truncatula ~5.2 million years ago, and the chromosomal fissions and fusions detected between the two genomes occurred during the divergence of the two species. In addition, we identified 489 resistance (R) genes and 82 and 85 candidate genes involved in the lignin and cellulose biosynthesis pathways, respectively. The near-complete and accurate diploid alfalfa reference genome obtained herein serves as an important complement to the recently assembled autotetraploid alfalfa genome and will provide valuable genomic resources for investigating the genomic architecture of autotetraploid alfalfa as well as for improving breeding strategies in alfalfa.

Frontal Eye Field Involvement in Color and Motion Feature-Based Attention: Single-Pulse Transcranial Magnetic Stimulation.

An object can have multiple attributes, and visual feature-based attention (FBA) is the process of focusing on a specific one of them. During visual FBA, the frontal eye field (FEF) is considered to be an important brain area related to the choice of attribute. However, the study of the FEF in FBA remains inadequate. We applied single-pulse transcranial magnetic stimulation (TMS) to the right FEF (rFEF), and designed two independent experimental FBA tasks that each involved two attributes (color and motion), to explore the action time of FEF and the spatial transmission of the FEF signal, respectively. The results of the first experiment showed that when TMS was applied to the rFEF at 100 ms after the target image stimulus began, the subjects' response time increased significantly compared with the response time in the control trials (in which TMS was applied to the vertex). This indicated that inhibiting the rFEF influenced the progress of visual FBA. The results confirm that the FEF is involved in the early stage of visual attention (at ~100 ms). In the second experiment, TMS was applied at 100 ms after the target image stimulus began. We analyzed the electroencephalogram (EEG) signal after TMS, and found that the electrode signal amplitudes for FC4 (which corresponded to the rFEF) were significantly correlated with the electrode signal amplitudes in the posterior regions. In addition, the amplitude rise of the posterior electrode signal lagged ~50 ms behind that of the FC4. Furthermore, for color and motion, different areas in the posterior brain region were involved in signal transmission. In this study, the application of single-pulse TMS was shown to provide a direct and effective method for research on the FEF, and the combination of TMS and EEG recordings allows a high degree of time resolution, which can provide powerful evidence for research on neural signal transmission.

[Studies on chemical components of Cirsium segestum].

Six chemical components were got from the alcohol extract of Cirsium segestum, their structures were identified by UV, IR, NMR, MS and EA. They were 5,7-dihydroxyflavone, baicalin, oleanolic acid, cholesterol, methyl ursolate and rutin. The first to the fifth of which were got from Cirsium segestum for the first time.

[The design for curing circuit used in Chinese acupuncture & moxibustion instrument].

In this paper, we introduce a multiple electro-stimulative pulse generator based on IC555, which can produce continuous, discontinuous, frequency modulated and amplitude modulated modes, and thus multiple-output, independent adjustment & control are realized. The principle block diagram and a reference circuit are presented too. This design adds acusetor curing function to BME-500 series Chinese Acupuncture & Moxibustion Instrument, making it a multifunctional therapy device of electro-acupuncture, massage and drug permeation Moxibustion Instrument.