RESUMEN
Frontal auditory evoked potentials (FAEPs) obtained as a response to the warning auditory stimulus of a contingent negative variation task from depth electrodes were investigated. The second, imperative stimulus was visual. Thirteen epileptic patients participated in the study. Records from 20 electrodes of 10 patients exhibited signs of local generation. They were localized in the motor cortices (7 cases), in the superior, medial, and inferior frontal gyri (7 cases), in the cingulate gyrus (5 cases), and in the nucleus caudatus (1 case). A typical FAEP from these generators consisted of three components: (i) first negative wave peaking at 99+/-13 ms; (ii) positive wave peaking at 181 +/- 21 ms; (iii) second negative wave peaking at 324 +/- 63 ms. In 11 generators no evoked activity to visual stimulus was observed; in the remaining 9 generators both auditory and visual stimuli evoked a response. FAEPs with very early onsets (onset latency below 20 ms) were found in three sites in the precentral gyrus.
Asunto(s)
Electroencefalografía/métodos , Lóbulo Frontal/fisiología , Estimulación Acústica , Adulto , Potenciales Evocados Auditivos/fisiología , Femenino , Giro del Cíngulo/fisiología , Humanos , Masculino , Persona de Mediana Edad , Tiempo de Reacción/fisiologíaRESUMEN
Intracranial recordings were obtained from three patients with intractable chronic pain who underwent analgesic electrical stimulation of the contralateral thalamus. Multilead electrode made it possible to record from several thalamic nuclei. The electrode was targeted into the ventroposterolateral (VPL) nucleus of the thalamus. During separate recording sessions, the following tests were performed: somatosensory evoked potentials (SEP) of the median or posterior tibial nerve, event-related cognitive potentials (auditory oddball P3 wave), readiness potential (RP) and contingent negative variation (CNV) using auditory warning (S1) and visual imperative (S2) stimuli. The movement accompanying potential (MAP), which was present in the VPL in all but one of the recordings, behaved as a far-field potential. Recordings obtained from the VPL confirmed its established role as a relay nucleus, processing somatosensory information to the primary somatosensory cortex. The VPL generated the 'thalamic' SEP, which was the only potential regularly recorded in this nucleus. In the recordings from one patient (No. 3), auditory and visual evoked potentials of the CNV protocol, peaking at approximately 300 ms, were obtained from the VPL and appeared to be generated in situ. Neither RP, CNV nor 'oddball' ERPs appeared in the VPL. From the pulvinar, only a visually evoked potential was recorded. Oddball P3, RP, CNV, and middle and long latency auditory and visual potentials (evoked in the CNV paradigm) appeared to be generated 'dorsally' to the VPL, probably in the nucleus posterolateralis (PL). This structure may therefore be involved in both the processing of afferent information and in cognitive operations.
Asunto(s)
Electroencefalografía , Potenciales Relacionados con Evento P300/fisiología , Movimiento/fisiología , Tálamo/fisiología , Anciano , Estimulación Eléctrica , Terapia por Estimulación Eléctrica , Electrodos Implantados , Electromiografía , Potenciales Evocados Somatosensoriales/fisiología , Humanos , Masculino , Persona de Mediana Edad , Manejo del Dolor , Núcleos Talámicos Posteriores/fisiologíaRESUMEN
We obtained rat brain slices (550-650 microm) that contained part of the frontoparietal cortex along with a portion of the thalamic ventrobasal complex (VB) and of the reticular nucleus (RTN). Maintained reciprocal thalamocortical connectivity was demonstrated by VB stimulation, which elicited orthodromic and antidromic responses in the cortex, along with re-entry of thalamocortical firing originating in VB neurons excited by cortical output activity. In addition, orthodromic responses were recorded in VB and RTN following stimuli delivered in the cortex. Spontaneous and stimulus-induced coherent rhythmic oscillations (duration = 0.4-3.5 s; frequency = 9-16 Hz) occurred in cortex, VB, and RTN during application of medium containing low concentrations of the K(+) channel blocker 4-aminopyridine (0.5-1 microM). This activity, which resembled electroencephalograph (EEG) spindles recorded in vivo, disappeared in both cortex and thalamus during application of the excitatory amino acid receptor antagonist kynurenic acid in VB (n = 6). By contrast, cortical application of kynurenic acid (n = 4) abolished spindle-like oscillations at this site, but not those recorded in VB, where their frequency was higher than under control conditions. Our findings demonstrate the preservation of reciprocally interconnected cortical and thalamic neuron networks that generate thalamocortical spindle-like oscillations in an in vitro rat brain slice. As shown in intact animals, these oscillations originate in the thalamus where they are presumably caused by interactions between RTN and VB neurons. We propose that this preparation may help to analyze thalamocortical synchronization and to understand the physiopathogenesis of absence attacks.
Asunto(s)
4-Aminopiridina/farmacología , Corteza Cerebral/citología , Corteza Cerebral/fisiología , Sincronización Cortical/efectos de los fármacos , Tálamo/citología , Tálamo/fisiología , Animales , Barbitúricos/farmacología , Estimulación Eléctrica , Epilepsia/inducido químicamente , Epilepsia/fisiopatología , Epilepsia Tipo Ausencia/inducido químicamente , Epilepsia Tipo Ausencia/fisiopatología , Antagonistas de Aminoácidos Excitadores/farmacología , Técnicas In Vitro , Ácido Quinurénico/farmacología , Vías Nerviosas , Periodicidad , Ratas , Ratas Wistar , Tiempo de Reacción/fisiología , Fases del Sueño/fisiologíaAsunto(s)
Corteza Cerebral/fisiopatología , Epilepsia/fisiopatología , N-Metilaspartato/fisiología , Receptores de N-Metil-D-Aspartato/fisiología , Calcio/fisiología , Canales de Calcio/fisiología , Técnicas de Cultivo , Electroencefalografía , Lóbulo Frontal/fisiopatología , Humanos , Psicocirugía , Lóbulo Temporal/fisiopatologíaRESUMEN
The responses to the glutamate agonist N-methyl-D-aspartate (NMDA) were studied in the sensori-motor cortex of rats with petit mal-like seizures. In a first study, the changes in extracellular concentration of calcium elicited through ionophoretic application of NMDA at various depths in the cortex were measured in vivo. The results show that in the cortex of epileptic rats the NMDA responses are much more widely distributed than in the cortex of control rats. In a second study, a current-source density analysis of the responses elicited through electrical stimulation of the white matter was performed in slices of neocortex in vitro. These findings show that the NMDA-dependent component of the synaptic responses are more widely distributed and of longer duration in the cortex of epileptic rats than in that of control rats. Taken together, these results suggest that in this model of absence epilepsy NMDA-dependent mechanisms are important in the triggering and maintenance of epileptic activity.
Asunto(s)
Epilepsia Tipo Ausencia/fisiopatología , Corteza Motora/efectos de los fármacos , N-Metilaspartato/farmacología , Corteza Somatosensorial/efectos de los fármacos , Aminoácidos/farmacología , Animales , Modelos Animales de Enfermedad , Estimulación Eléctrica , Potenciales Evocados Somatosensoriales/efectos de los fármacos , Técnicas In Vitro , Iontoforesis , Masculino , N-Metilaspartato/antagonistas & inhibidores , Ratas , Ratas Endogámicas/genética , Sinapsis/efectos de los fármacosRESUMEN
The ionic mechanisms underlying the action of excitatory amino acids were investigated in the rat motor cortex. Ion-selective microelectrodes were attached to micropipettes such that their tips were very close and local changes in extracellular concentration of sodium, calcium, and potassium ions elicited through ionophoretic applications of glutamate (Glu) and of its agonists N-methyl-D-aspartate (NMDA), quisqualate (Quis), and kainate (Ka) were measured. These agents produced moderate increases in [K+]o (up to 13 mM) but, in contrast, substantial tetrodotoxin-insensitive decreases in [Na+]o (maximally of 60 mM). NMDA-induced sodium responses could be blocked by manganese, while the Quis- and Ka-induced responses were not. Quis and Ka produced increases in [Ca2+]o or biphasic responses while NMDA, even with small doses, induced each time drastic decreases in [Ca2+]o (maximally of 1.15 mM), which could be attenuated or blocked by manganese but not by organic calcium channel blockers. NMDA responses could be abolished by reduced doses of 2-amino-phosphonovalerate. The largest Glu- and NMDA-induced calcium responses were observed in the superficial cortical layers, but such maxima disappeared after selective degeneration of pyramidal tract neurons. All amino acids produced sizeable reductions in the extracellular space volume. The following can be concluded. (i) All the excitatory amino acids tested induce an increased permeability to sodium and potassium ions. (ii) In addition, the NMDA-operated channels have specifically a large permeability for calcium, although calcium ions contribute only by less than 10% to the NMDA-induced inward currents.(ABSTRACT TRUNCATED AT 250 WORDS)
Asunto(s)
Aminoácidos Diaminos/farmacología , Cationes/metabolismo , Corteza Cerebral/metabolismo , Receptores de Neurotransmisores/fisiología , Potenciales de Acción/efectos de los fármacos , Animales , Ácido Aspártico/análogos & derivados , Ácido Aspártico/antagonistas & inhibidores , Ácido Aspártico/farmacología , Calcio/metabolismo , Bloqueadores de los Canales de Calcio/farmacología , Corteza Cerebral/efectos de los fármacos , Espacio Extracelular/metabolismo , Microelectrodos , Corteza Motora/metabolismo , N-Metilaspartato , Potasio/metabolismo , Ratas , Ratas Endogámicas , Sodio/metabolismo , Tetrodotoxina/farmacologíaRESUMEN
Extracellular free sodium (Na+)o and calcium (Ca2+)o concentration changes were measured in the rat motor cortex, using ion-selective microelectrodes. During ionophoretic applications of excitatory amino acids, decreases in (Na2+)o and in (Ca2+)o were observed. Ca2+ signals were not or very little modified by applications of tetrodotoxin while Na+ signals were slightly depressed, up to 20%. Laminar profile analysis revealed that, while the magnitude of Na+ signals was rather constant throughout the cortex, Ca2+ signals were largest in upper cortical layers. Lesioning and pharmacological experiments indicated that the corresponding permeabilities were most probably located on apical dendrites of pyramidal tract neurons. The relative amplitude of Na+ and Ca2+ signals induced by the release of the glutamate agonists N-methyl-D-aspartate, quisqualate and kainate and the shape of the laminar profile of such responses indicated that different ionic permeabilities located on different neurons underlie such responses. Similar experiments performed on chronic epileptogenic motor foci in rats indicated that the amino acid-induced ionic responses were altered. The significance of such alterations for epileptogenesis is discussed.
Asunto(s)
Aminoácidos/farmacología , Epilepsia/fisiopatología , Corteza Motora/fisiopatología , Animales , Ácido Aspártico/análogos & derivados , Ácido Aspártico/farmacología , Calcio/fisiología , Cobalto/farmacología , Conductividad Eléctrica , Glutamatos/farmacología , Ácido Kaínico/farmacología , N-Metilaspartato , Oxadiazoles/farmacología , Tractos Piramidales/fisiopatología , Ácido Quiscuálico , Ratas , Sodio/fisiologíaRESUMEN
Changes in [Ca2+]o and [K+]o were measured in the sensorimotor cortex of cats during repetitive electrical stimulation and during pentetrazol induced epileptiform activity. Repetitive stimulation of the thalamic ventrobasal complex (VB) or of the cortical surface (CS) caused decreases in [Ca2+]o by up to 0.45 mM and increases in [K+]o by up to 7 mM. Maximum reductions of [Ca2+]o delta [Ca2+]o were found in depths of 100 to 300 micrometers below cortical surface, while rises in [K+]o were largest in depths of 600 to 1000 micrometers dependent on stimulation site. At depths below 700-900 micrometers increases in [K+]o were often accompanied by rises in [Ca2+]o of about 0.2 mM. Pentetrazol (PTZ) when injected at doses of 25 to 40 mg/kg body weight induced spontaneous seizure activity, which was in about 40% preceded by a slight fall of baseline [Ca2+]o. Repetitive stimulation and spontaneous seizures resulted in delta [Ca2+]o of up to 0.6 mM, whereas rises in [K+]o remained limited to a 'ceiling level' of about 10 mM. After PTZ application, peak delta [Ca2+]o were found at the same recording sites, but, in contrast to normal cortex, decreases in [Ca2+]o were observed in all cortical layers. The enhanced Ca2+-signals after PTZ application and the observed reduction of [Ca2+]o before seizure onset suggest that PTZ utilizes Ca2+-dependent mechanisms to initiate seizure activity.