RESUMEN
OBJECTIVE: To research the disperse behavior of many Chinese medicine decoctions. METHODS: Through the analysis of the dispersible attributes of 22 kinds of Chinese medicine decoctions including Radix Salviae Miltiorrhizae by means of turbidity, ultramicroscope and TEM, we found that a lot of nanometer particles existed in these decoctions after 4000 r/min centrifugation. Based on the model of Radix Salviae Miltiorrhizae, we analyzed the influence on the loss of effective ingredients by way of centrifugation, alcohol precipitation, flocculation and salting-out edulcoration. RESULTS: The removal of infinite nanometer particles from these decoctions led to the greater loss of water-soluble and fat-soluble ingredients,and the latter accounts for the major loss. CONCLUSION: Oral liquid preparation of traditional Chinese medicine is a kind of nano-pharmaceutics with nanometer particles dispersed in the water as the carriers of effective ingredients in medicine.
Asunto(s)
Nanopartículas/química , Extractos Vegetales/química , Plantas Medicinales/química , Química Farmacéutica , Estabilidad de Medicamentos , Microscopía Electrónica de Transmisión , Nanopartículas/ultraestructura , Tamaño de la Partícula , Extractos Vegetales/análisis , Extractos Vegetales/aislamiento & purificación , Control de Calidad , Salvia miltiorrhiza/química , Solubilidad , Tecnología FarmacéuticaRESUMEN
OBJECTIVE: To investigate the mechanisms of the oil-in-oil nanoemulsions transport through the gastrointestinal tract and the transport efficiency changed with its different particle size in the lymphatic channels. METHOD: The behavior of nanoemulsions in vivo and their absorption via lymph after oral administration was investigated, with the transport efficiency and absorption pathway of nanoemulsions clarified by lymph duct cannulation in rats. RESULT: It suggested about 36.8% of puerarin nanoemulsions was transported into systematic circulation via lymph. Nanoparticles with different size absorbed by the lymphatic channels varied as the degree of transportion. CONCLUSION: The degree of absorption and particle transport is inversely proportional to the size.
Asunto(s)
Isoflavonas/farmacocinética , Absorción , Animales , Transporte Biológico , Emulsiones , Femenino , Isoflavonas/administración & dosificación , Linfa/metabolismo , Masculino , Nanopartículas , Tamaño de la Partícula , Ratas , Ratas Sprague-DawleyRESUMEN
The main purpose of this study was to prepare puerarin microemulsion system to improve oral bioavailability of puerarin. The microemulsion formulations were prepared using soybean oil, soybean lecithin/ethyl lactate (1:1), and 1,2-propanediol/water. The presence of microemulsion regions were investigated by pseudo-ternary phase diagrams. The droplet size of microemulsion was characterized by photo-correlation spectroscopy. In vivo pharmacokinetic study was conducted in mice, and the results indicated that AUC(0-->infinity) was 15.82-fold higher than that of puerarin suspension upon oral administration. Particles of puerarin microemulsion were round and homogeneous. Puerarin microemulsion also showed good stability. These studies showed that microemulsion system of puerarin might be promising vehicles for the peroral delivery of puerarin.