Tuina Intervention in Sodium Monoiodoacetate Injection-Induced Rat Model of Knee Osteoarthritis.

Knee osteoarthritis (KOA), a common degenerative joint disorder, is characterized by chronic pain and disability, which can progress to irreparable structural damage of the joint. Investigations into the link between articular cartilage, muscles, synovium, and other tissues surrounding the knee joint in KOA are of great importance. Currently, managing KOA includes lifestyle modifications, exercise, medication, and surgical interventions; however, the elucidation of the intricate mechanisms underlying KOA-related pain is still lacking. Consequently, KOA pain remains a key clinical challenge and a therapeutic priority. Tuina has been found to have a regulatory effect on the motor, immune, and endocrine systems, prompting the exploration of whether Tuina could alleviate KOA symptoms, caused by the upregulation of inflammatory factors, and further, if the inflammatory factors in skeletal muscle can augment the progression of KOA. We randomized 32 male Sprague Dawley (SD) rats (180-220 g) into four groups of eight animals each: antiPD-L1+Tuina (group A), model (group B), Tuina (group C), and sham surgery (group D). For groups A, B, and C, we injected 25 µL of sodium monoiodoacetate (MIA) solution (4 mg MIA diluted in 25 µL of sterile saline solution) into the right knee joint cavity, and for group D, the same amount of sterile physiological saline was injected. All the groups were evaluated using the least to most stressful tests (paw mechanical withdrawal threshold, paw withdrawal thermal latency, swelling of the right knee joint, Lequesne MG score, skin temperature) before injection and 2, 9, and 16 days after injection.

Effect of circulation and micro-aeration on sludge characteristics and microbial community in an ABR for treating traditional Chinese medicine wastewater.

The effects of circulation reflux and micro-aeration on the performance of a modified anaerobic baffled reactor (ABR) for treatment of traditional Chinese medicine (TCM) wastewater were evaluated. The characteristics of anaerobic sludge and microbial community structure in the modified ABR were also investigated. The results indicated that with conditions of reflux ratio of 1, reflux ratio of 2, reflux ratio of 2 with micro-aeration, and reflux ratio of 3, the modified ABR achieved an average COD removal efficiency of 90%, 87.7%, 87.8%, and 88.4%, respectively. In addition, the NH3-N average removal efficiency was 45.1%, 50%, 55.9%, and 55.4%, respectively. The analysis of excitation-emission matrix (EEM) fluorescence spectra of soluble microbial products (SMP) and extracellular polymeric substances (EPS) showed that there were tyrosine-like, aromatic protein-like, and coenzyme F420 substances in the sludge. The EPS were analysed by the Fourier transform infrared spectroscopy (FTIR), which showed that aromatic compounds were partially degraded, while the protein and polysaccharide compounds increased in each compartment of the modified ABR. Interestingly, the microbial community of anaerobic sludge analysis results showed that Chloroflexi was the dominant in the first, third and fourth compartments. Meanwhile, Levilinea and Methanothrix were the dominant species in the first and third compartments at the genus level.

Effects of hydraulic retention time on the performance and microbial community of an anaerobic baffled reactor-bioelectricity Fenton coupling reactor for treatment of traditional Chinese medicine wastewater.

The aim of the present paper was to investigate the effects of hydraulic retention time (HRT) on the performance and microbial community dynamics of an anaerobic baffled reactor-bioelectricity Fenton (ABR-BEF) coupling reactor for treating traditional Chinese medicine (TCM) wastewater. The results show that the average removal of chemical oxygen demand (COD) and NH3-N at HRTs of 24 h and 18 h were high (>90% and >70%, respectively), but decreased to about 40% and 30% when operating at 12 h HRT. For the electrical production performance, the maximum power density was 196.86 mW/m3 at a HRT of 18 h. Methanomicrobia was the dominant archaea in the coupling reactor and the relative abundance of Methanothrix and Methanolinea increased with decreasing HRT. For the bacteria, the relative abundance of Planctomycetia significantly decreased with a short HRT; however, Anaerolineaceae was always the dominant bacterial taxa, which could guarantee efficient treatment of TCM wastewater.

Performance of a novel ABR-bioelectricity-Fenton coupling reactor for treating traditional Chinese medicine wastewater containing catechol.

In this study, a novel anaerobic baffled reactor-bioelectricity-Fenton (ABR-BEF) coupling reactor, combining an ABR, microbial fuel cell (MFC), and Fenton system, was used to treat traditional Chinese medicine (TCM) wastewater containing catechol. The bio-electrochemical degradation of the catechol reached 99.7% after 8 h at dissolved oxygen (DO) concentration of 4 mg/L in the cathodic chamber. The removal rates of chemical oxygen demand (COD) reached 91.7%, when the ratio rate was 1 and the DO concentration was 4 mg/L. Moreover, the maximum open-circuit voltage and power density of the coupling reactor reached 424.9 mV and 77.1 mW/m3, respectively. According to the PICRUSt analysis, carbohydrate metabolism took up the most abundant function of metabolism and the enrichment of membrane transporters may relieve TCM wastewater toxicity. These results suggest that the ABR-BEF coupling reactor could be applied as an efficient approach to treat TCM wastewater.

[Efficiency and Microecology of a Soil Infiltration System with High Hydraulic Loading for the Treatment of Swine Wastewater].

The swine wastewater after treated with an anaerobic reactor was used to investigate the removal efficiency of COD, ammonia nitrogen, and total nitrogen in an ecological high hydraulic loading soil infiltration system. Meanwhile, the microbial community structure and the contents of the catalase, urease, and nitrate reductase were analyzed. The results showed that with the hydraulic load of 11 cm·d-1 and the influent COD concentration of 700 mg·L-1, the removal rate of COD was 78.8%, 63.0%, and 92.6%for the first land infiltration column, the secondary land infiltration column, and the total system, respectively. When the hydraulic load increased to 22 cm·d-1; the total removal rate of COD was also above 90.0%. The system was more significant for ammonia nitrogen removal. The removal rate of ammonia nitrogen reached approximately 99%. The contents of catalase were 1.899, 0.990, and 0.323 mL·g-1 at the upper, middle, and bottom sections of the system, respectively. The organic matter in the swine wastewater was removed at the upper and middle of the system. On the secondary soil infiltration system, the content of nitrate reductase was 3.453, 3.831, and 1.971 mL·g-1, respectively. Denitrification mainly occurred in the upper and middle of the secondary soil infiltration system. Gram Negative and Gram Positive bacteria were given priority in the soil infiltration system. Especially, arbuscular mycorrhizal fungi and Actinomycetes dominated the secondary soil infiltration system. The system provides a guarantee for the removal of the refractory organic compounds from the swine wastewater.

Effect of heterogeneous Fenton-like pre-treatment on anaerobic granular sludge performance and microbial community for the treatment of traditional Chinese medicine wastewater.

The effect of a heterogeneous Fenton-like pre-treatment on the anaerobic processes, characteristics and microbial community of sludge was investigated for traditional Chinese medicine (TCM) wastewater containing rhein. When the concentrations of rhein were 50mg/L and 100mg/L, the toxic effect was physiological toxicity for anaerobic granular sludge. Using a single double circle (DC) reactor for the treatment of TCM wastewater containing rhein at concentrations of 15-20mg/L, the chemical oxygen demand (COD) removal rate was 69%, and coenzyme F420 was nearly undetectable in the 3D-excitation-emission matrix (EEM) spectra of soluble microbial products (SMP). The abundances of Methanoregula, Methanobacterium, Methanosphaerula were only 5.57%, 2.39% and 1.08% in the DC reactor, respectively. TCM wastewater containing rhein could be successfully treated by the combination of the heterogeneous Fenton-like pre-treatment and the DC reactor processes, and the COD removal rate reached 95%. Meanwhile, the abundances of Methanoregula, Methanobacterium, Methanosphaerula increased to 22.5%, 18.5%, and 13.87%, respectively. For the bacterial community, the abundance of Acidobacteria_Gp6 decreased from 6.99% to 1.07%, while the abundances of Acidobacteria_Gp1 and Acidobacteria_Gp2 increased from 1.61% to 6.55% and from 1.28% to 5.87%, respectively.