RESUMEN
Four new taraxastane-type triterpenoids acids 3ß,22α-dihydroxy-20-taraxasten-30-oic acid (1), 3ß-hydroxy-22-oxo-20-taraxasten-30-oic acid (2), 3-oxo-22α-hydroxy-20- taraxasten-30-oic acid (3), and 3ß,19ß-dihydroxy-20-taraxasten-30-oic acid (4) were isolated and characterized from Cirsium setosum (Willd.) MB. Their structures were determined by the combination of 1D and 2D NMR experiments ((1)H-(1)HCOSY, HSQC, HMBC and ROESY) and mass spectrometry. Compound 2 exhibited potent selective cytotoxicity against human ovarian cancer cell line A2780 with an IC50 value of 3.9 µM.
Asunto(s)
Cirsium/química , Triterpenos/aislamiento & purificación , Medicamentos Herbarios Chinos/química , Humanos , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular , Triterpenos/química , Triterpenos/farmacologíaRESUMEN
OBJECTIVE: To study the extraction technology of flavonoids from Cynomorium songaricum by supercritical CO2 extraction. METHODS: The effects of pressure, temperature, time, concentration of alcohol, dosage of chemical preparation, flux of CO2 and particle size were studied by single factor analysis and orthogonal test. RESULTS: The optimized conditions were as follows: particle size 60 - 80 sieve mesh, the pressure was 30 MPa, the temperature was 50 degrees C, the time was 75 min, concentration of alcohol was 50%, entrainment rate was 8%, flux of CO2 was 5 mL/min. The total flavonoids yield could reach 21.18% under the above conditions. CONCLUSION: This method is simple, rapid and higher extraction yield, so it is suitable for the extraction of flavonoids from Cynomorium songaricum.