RESUMEN
Matrine, a alkaloid of the root of Sophora flavescens, has multiple protective effects on the cardiovascular system including cardiac arrhythmias. However, the molecular and ionic mechanisms of matrine have not been well investigated. Our study aimed at to shed a light on the issue to investigate the antiarrhythmic effects of matrine by using ouabain to construct an arrhythmic model of cardiomyocytes. In this experiment, matrine significantly and dose-dependently increased the doses of ouabain required to induce cardiac arrhythmias and decreased the duration of arrhythmias in guinea pigs. In cardiomyocytes of guinea pigs, ouabain 10 µM prolonged action potential duration by 80% (p<0.05) and increased L-type Ca(2+) currents and Ca(2+) transients induced by KCl (p<0.05). Matrine 100 µM shortened the prolongation of APD and prevented the increase of L-type Ca(2+) currents and Ca(2+) transients induced by ouabain. Taken together, these findings provide the first evidence that matrine possessed arrhythmogenic effect of ouabain by inhibiting of L-type Ca(2+) currents and Ca(2+) overload in guinea pigs.
Asunto(s)
Alcaloides/farmacología , Antiarrítmicos/farmacología , Ouabaína/farmacología , Quinolizinas/farmacología , Sophora/química , Potenciales de Acción/efectos de los fármacos , Animales , Arritmias Cardíacas/inducido químicamente , Arritmias Cardíacas/tratamiento farmacológico , Calcio/metabolismo , Canales de Calcio Tipo L/metabolismo , Células Cultivadas , Modelos Animales de Enfermedad , Relación Dosis-Respuesta a Droga , Cobayas , Miocitos Cardíacos/efectos de los fármacos , Miocitos Cardíacos/metabolismo , MatrinasRESUMEN
OBJECTIVE: To provide the identification basis for Mongolian medicine Potentilla glabra. METHODS: Macroscopic and microscopic identification were applied to observe macroscopic, histological, superficial, and powder characteristics of its stems and leaves. RESULTS: The following characteristics were observed: The cork layer sandwiched with sclerenchyma ring which was main composed of fibers, accompanied by stone cells and large cell layer; Catheter with fiber existed in xylem; Beaded thickened anticlinal wall in leaf epidermal cell; Stomatal infinitive; Small clusters of calcium oxalate crystal and different type of leaf transverse section. CONCLUSION: These characteristics can provide evidences for the identification and quality control of Potentilla glabra.