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1.
Int J Mol Sci ; 16(8): 19291-307, 2015 Aug 14.
Artículo en Inglés | MEDLINE | ID: mdl-26287178

RESUMEN

Superparamagnetic iron oxide nanoparticles (SPIONs) are frequently used for drug targeting, hyperthermia and other biomedical purposes. Recently, we have reported the synthesis of lauric acid-/albumin-coated iron oxide nanoparticles SEON(LA-BSA), which were synthesized using excess albumin. For optimization of magnetic treatment applications, SPION suspensions need to be purified of excess surfactant and concentrated. Conventional methods for the purification and concentration of such ferrofluids often involve high shear stress and low purification rates for macromolecules, like albumin. In this work, removal of albumin by low shear stress tangential ultrafiltration and its influence on SEON(LA-BSA) particles was studied. Hydrodynamic size, surface properties and, consequently, colloidal stability of the nanoparticles remained unchanged by filtration or concentration up to four-fold (v/v). Thereby, the saturation magnetization of the suspension can be increased from 446.5 A/m up to 1667.9 A/m. In vitro analysis revealed that cellular uptake of SEON(LA-BSA) changed only marginally. The specific absorption rate (SAR) was not greatly affected by concentration. In contrast, the maximum temperature Tmax in magnetic hyperthermia is greatly enhanced from 44.4 °C up to 64.9 °C by the concentration of the particles up to 16.9 mg/mL total iron. Taken together, tangential ultrafiltration is feasible for purifying and concentrating complex hybrid coated SPION suspensions without negatively influencing specific particle characteristics. This enhances their potential for magnetic treatment.


Asunto(s)
Ácidos Láuricos/química , Nanopartículas de Magnetita/química , Albúmina Sérica Bovina/química , Ultrafiltración/métodos , Animales , Bovinos , Coloides/química , Coloides/aislamiento & purificación , Humanos , Hipertermia Inducida , Células Jurkat , Ácidos Láuricos/aislamiento & purificación , Magnetismo , Albúmina Sérica Bovina/aislamiento & purificación , Propiedades de Superficie
2.
Nanomedicine (Lond) ; 7(3): 447-57, 2012 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-22385201

RESUMEN

The term 'nanomedicine' refers to the use of nanotechnology in the treatment, diagnosis and monitoring of diseases. Magnetic drug targeting is a particularly promising application in this field. The goal of the carrier systems involved is to achieve active enrichment of effective substances in diseased tissue. Numerous nanosystems can be used as carriers, but magnetic iron oxide nanoparticles are particularly important. On the one hand, the particles serve as carriers for the active substance, while on the other hand they can also be visualized using conventional imaging techniques and can therefore be used for 'theranostic' purposes. They can also be used in hyperthermia, another important pillar of nanomedicine. Both procedures are intended to lead to specific forms of treatment, which is of medical and economic relevance in view of the increasing numbers of cancer patients worldwide. This study offers a brief overview of current developments in medical applications for magnetic nanoparticles in cancer therapy.


Asunto(s)
Antineoplásicos/administración & dosificación , Sistemas de Liberación de Medicamentos , Imanes/química , Nanopartículas/química , Nanopartículas/uso terapéutico , Neoplasias/terapia , Animales , Compuestos Férricos/química , Humanos , Hipertermia Inducida/métodos , Nanomedicina/métodos
3.
Gene ; 376(2): 184-91, 2006 Jul 19.
Artículo en Inglés | MEDLINE | ID: mdl-16624504

RESUMEN

The human gene deleted in malignant brain tumors 1 (DMBT1) is considered to play a role in tumorigenesis and pathogen defense. It encodes a protein with multiple scavenger receptor cysteine-rich (SRCR) domains, which are involved in recognition and binding of a broad spectrum of bacterial pathogens. The SRCR domains are encoded by highly homologous repetitive exons, whose number in humans may vary from 8 to 13 due to genetic polymorphism. Here, we characterized the porcine DMBT1 gene on the mRNA and genomic level. We assembled a 4.5 kb porcine DMBT1 cDNA sequence from RT-PCR amplified seminal vesicle RNA. The porcine DMBT1 cDNA contains an open reading frame of 4050 nt. The transcript gives rise to a putative polypeptide of 1349 amino acids with a calculated mass of 147.9 kDa. Compared to human DMBT1, it contains only four N-terminal SRCR domains. Northern blotting revealed transcripts of approximately 4.7 kb in size in the tissues analyzed. Analysis of ESTs suggested the existence of secreted and transmembrane variants. The porcine DMBT1 gene spans about 54 kb on chromosome 14q28-q29. In contrast to the characterized cDNA, the genomic BAC clone only contained 3 exons coding for N-terminal SRCR domains. In different mammalian DMBT1 orthologs large interspecific differences in the number of SRCR exons and utilization of the transmembrane exon exist. Our data suggest that the porcine DMBT1 gene may share with the human DMBT1 gene additional intraspecific variations in the number of SRCR-coding exons.


Asunto(s)
Neoplasias Encefálicas/genética , Eliminación de Gen , Porcinos/genética , Proteínas Supresoras de Tumor/química , Proteínas Supresoras de Tumor/genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Cromosomas de los Mamíferos , Cisteína/química , ADN Complementario/genética , Exones , Etiquetas de Secuencia Expresada , Mutación del Sistema de Lectura , Duplicación de Gen , Variación Genética , Genoma , Intrones , Datos de Secuencia Molecular , Peso Molecular , Sistemas de Lectura Abierta , Polimorfismo Genético , Unión Proteica , Estructura Terciaria de Proteína , Empalme del ARN , ARN Mensajero/genética , Proteínas Supresoras de Tumor/metabolismo
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