RESUMEN
Osteosarcoma is the most common primary malignant tumor of bone usually occurring in young adolescent and children. This disease has a poor prognosis, because of the metastases in the period of tumor progression, which are usually developed previous to the clinical diagnosis. In this paper, a 2000-year-old ancient bone remain with osteogenic sarcoma was analyzed searching for tumor biomarkers which are closely related to this disease. After a specific extraction SDS-PAGE gel electrophoresis followed by tryptic digestion was performed. After the digestion the samples were measured using MALDI TOF/TOF MS. Healthy bone samples from same archaeological site were used as control samples. Our results show that in the pathological skeletal remain several well known tumor biomarkers are detected such as annexin A10, BCL-2-like protein, calgizzarin, rho GTPase-activating protein 7, HSP beta-6 protein, transferrin and vimentin compared to the control samples. The identified protein biomarkers can be useful in the discovery of malignant bone lesions such as osteosarcoma in the very early stage of the disease from paleoanthropological remains.
Asunto(s)
Biomarcadores de Tumor/metabolismo , Huesos/metabolismo , Regulación Neoplásica de la Expresión Génica/genética , Osteosarcoma/historia , Osteosarcoma/metabolismo , Arqueología , Biomarcadores de Tumor/genética , Huesos/patología , Electroforesis en Gel de Poliacrilamida , Historia Antigua , Humanos , Hungría , Espectrometría de Masas , Osteosarcoma/genéticaRESUMEN
The purpose of this study was to analyse a skeleton (adult female, 25-30 years) that presented evidence of tuberculous spondylitis. The skeleton, dated from the Roman Period (III-VI centuries), was excavated near the town of Győr, in western Hungary. The skeleton was examined by gross observation supplemented with mycolic acid and proteomic analyses using MALDI-TOF/TOF tandem mass spectrometry. The biomolecular analyses supported the morphological diagnosis.
Asunto(s)
Adulto , Femenino , Historia Antigua , Humanos , Tuberculosis de la Columna Vertebral/historia , Hungría , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción , Tuberculosis de la Columna Vertebral/patologíaRESUMEN
The purpose of this study was to analyse a skeleton (adult female, 25-30 years) that presented evidence of tuberculous spondylitis. The skeleton, dated from the Roman Period (III-VI centuries), was excavated near the town of Gyor, in western Hungary. The skeleton was examined by gross observation supplemented with mycolic acid and proteomic analyses using MALDI-TOF/TOF tandem mass spectrometry. The biomolecular analyses supported the morphological diagnosis.