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1.
J Biol Chem ; 281(17): 11560-8, 2006 Apr 28.
Artículo en Inglés | MEDLINE | ID: mdl-16505484

RESUMEN

We identified the gene encoding chondroitin-glucuronate C5-epimerase (EC 5.1.3.19) that converts D-glucuronic acid to L-iduronic acid residues in dermatan sulfate biosynthesis. The enzyme was solubilized from bovine spleen, and an approximately 43,000-fold purified preparation containing a major 89-kDa candidate component was subjected to mass spectrometry analysis of tryptic peptides. SART2 (squamous cell carcinoma antigen recognized by T cell 2), a protein with unknown function highly expressed in cancer cells and tissues, was identified by 18 peptides covering 26% of the sequence. Transient expression of cDNA resulted in a 22-fold increase in epimerase activity in 293HEK cell lysate. Moreover, overexpressing cells produced dermatan sulfate chains with 20% of iduronic acid-containing disaccharide units, as compared with 5% for mock-transfected cells. The iduronic acid residues were preferentially clustered in blocks, as in naturally occurring dermatan sulfate. Given the discovered identity, we propose to rename SART2 (Nakao, M., Shichijo, S., Imaizumi, T., Inoue, Y., Matsunaga, K., Yamada, A., Kikuchi, M., Tsuda, N., Ohta, K., Takamori, S., Yamana, H., Fujita, H., and Itoh, K. (2000) J. Immunol. 164, 2565-2574) with a functional designation, chondroitin-glucuronate C5-epimerase (or DS epimerase). DS epimerase activity is ubiquitously present in normal tissues, although with marked quantitative differences. It is highly homologous to part of the NCAG1 protein, encoded by the C18orf4 gene, genetically linked to bipolar disorder. NCAG1 also contains a putative chondroitin sulfate sulfotransferase domain and thus may be involved in dermatan sulfate biosynthesis. The functional relation between dermatan sulfate and cancer is unknown but may involve known iduronic acid-dependent interactions with growth factors, selectins, cytokines, or coagulation inhibitors.


Asunto(s)
Antígenos de Neoplasias/química , Carbohidrato Epimerasas/metabolismo , Proteínas de Unión al ADN/química , Dermatán Sulfato/biosíntesis , Proteínas de Neoplasias/química , Secuencia de Aminoácidos , Animales , Antígenos de Neoplasias/metabolismo , Carbohidrato Epimerasas/genética , Carbohidrato Epimerasas/aislamiento & purificación , Bovinos , Células Cultivadas , ADN Complementario , Proteínas de Unión al ADN/metabolismo , Humanos , Ácido Idurónico/metabolismo , Riñón/metabolismo , Espectrometría de Masas , Datos de Secuencia Molecular , Músculos/metabolismo , Proteínas de Neoplasias/metabolismo , Ratas , Homología de Secuencia de Aminoácido , Bazo/enzimología
2.
Glycobiology ; 15(12): 1277-85, 2005 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-16118286

RESUMEN

The chondroitin/dermatan sulfate proteoglycans (CS/DSPGs), biglycan, decorin, and versican play several important roles in extracellular matrix influencing matrix organization, cell proliferation, and recruitment. Moreover, they bind and regulate growth factors in the extracellular matrix. We have previously shown that cultured human lung fibroblasts treated with transforming growth factor-beta (TGF-beta) alone or in combination with epidermal growth factor and platelet-derived growth factor, increase the production of these PGs. In this report, we describe that the structure of their galactosaminoglycan side chains is altered, albeit there is no alteration of polysaccharide length. The findings showed that iduronic acid content is reduced by 50% in decorin and biglycan, whereas 4-O-sulfation is increased 2-fold in versican. To unravel the mechanism behind these changes, the activities of chondroitin C-5 epimerase and of O-sulfotransferases in cellular fractions prepared from fibroblasts were quantitated, and transcript levels of the relevant sulfotransferases were measured by real time polymerase chain reaction (RT-PCR). The C-5 epimerase activity was reduced by 25% in TGF-beta1 treated cells and 50% in fibroblasts treated with the growth factor combination. No change in activity in dermatan 4-O sulfotransferase was observed, and only a minor decrease in dermatan 4-O-sulfotransferase-1 (D4ST-1) mRNA was observed. On the other hand, chondroitin 4-O sulfotransferase activity increased 2-fold upon TGF-beta1 treatment and 3-fold upon treatment with the growth factor combination. This is in agreement with a 2-fold up-regulation of chondroitin-4-O-sulfotransferase 1 (C4ST-1) mRNA, and no changes in chondroitin-4-O-sulfotransferase 2 (C4ST-2) mRNA. Thus, cellular activity and transcript level correlated well with the changes in the structure of the dermatan/chondroitin sulfate chains.


Asunto(s)
Condroitín/química , Citocinas/metabolismo , Dermatán Sulfato/química , Regulación de la Expresión Génica , Factor de Crecimiento Transformador beta/metabolismo , Carbohidrato Epimerasas/química , Línea Celular , Células Cultivadas , Sulfatos de Condroitina/química , Cartilla de ADN/química , ADN Complementario/metabolismo , Decorina , Disacáridos/química , Factor de Crecimiento Epidérmico/metabolismo , Matriz Extracelular/metabolismo , Proteínas de la Matriz Extracelular/química , Fibroblastos/metabolismo , Humanos , Ácido Idurónico/química , Microsomas/metabolismo , Polímeros/química , Polisacáridos/química , Proteoglicanos/química , ARN/química , ARN Mensajero/metabolismo , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Sulfotransferasas/química , Sulfotransferasas/metabolismo , Factor de Crecimiento Transformador beta/química , Factor de Crecimiento Transformador beta1 , Regulación hacia Arriba
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