RESUMEN
Since ancient times the oil from date palm pits (Phoenix dactylifera L.) has been used to heal wounds. In order to prove this traditional usage of the pits, this oil was extracted from the pits of the Tunisian cultivar 'Alig' and its physico-chemical properties and the chemical composition were evaluated. The fatty acid profile, evidenced by GC, allowed to classify this oil as an oleic-myristic acid oil with a clear abundance of oleic acid (53.66 %). 1 H and 13 C-NMR as well as FT-IR analyses confirmed the presence of fatty acids in triglyceride forms. Furthermore, inâ vivo wound healing activity of a cream formulated from the extracted oil was performed, for the first time, using a rat model and was compared to placebo cream and a commercial formulation, MEBO®. This study showed that the test cream promoted the healing of pressure ulcers better than the placebo cream and the MEBO® ointment. The results showed that this vegetable oil is able to improve the healing of infected wounds in rats, thus supporting its traditional use. The contribution of the main oleic, linoleic and myristic acids that can be derived from enzymatic hydrolysis to the healing activity of the whole pits oil was predicted by in silico study and the calculated pharmacokinetics parameters.
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Phoeniceae , Ratas , Animales , Phoeniceae/química , Simulación del Acoplamiento Molecular , Espectroscopía Infrarroja por Transformada de Fourier , Aceites de Plantas/química , Cicatrización de Heridas , Ácidos Grasos/análisis , Ácido OléicoRESUMEN
Allium roseum is one of the medicinal plants of the Liliaceae family, widely used in the food industry and traditional medicine. It is known for its various biological properties, such as its antioxidant, antiviral, antidiabetic, and anti-inflammatory activities. The present work aims to extract the polysaccharides from Allium roseum leaves and evaluate their antioxidant activities and hepatoprotective effects in vivo. Three polysaccharides from the leaves of Allium roseum were sequentially extracted in three media: water, chelating, and basic, respectively. They were characterized by size exclusion chromatography, gas chromatography mass spectrometry, FTIR-ATR, and NMR spectroscopy (1D and 2D). The different polysaccharides principally consist of glucose, galactose, mannose, rhamnose, xylose, and galacturonic acid. The antioxidant activity and hepatoprotective effect of the extracts against Cd-caused oxidative stress in liver mouse were tested. Cd treatment, during 24 h, enhanced significantly lipid peroxidation by a high production of malondyaldehyd (MDA) and superoxide dismutase (SOD) activity. In contrast, catalase activity (CAT) was decreased after the same period of exposure to the metal. The polysaccharides pre-treatment improved the antioxidant defense system to a great degree, mainly explained by the modulating levels of oxydative stress biomarkers (MDA, SOD, and CAT). This research clearly shows that Allium roseum polysaccharides, especially those extracted in aqueous medium, can be used as natural antioxidants with hepatoprotective properties.
RESUMEN
The aim of this study was to investigate antimicrobial and antioxidant activities of different fractions obtained from edible Tunisian Ziziphus Lotus leaves of Tozeur region. Different organic extracts were tested: cyclohexane, dichloromethane, ethyl acetate, n-butanol and water. Bio-guided fractionation revealed that dichloromethane fraction is the most active against S. aureus and Methicillin-resistant S. aureus strains. Moreover, this fraction showed the highest antileishmanial activity with IC50 values of 20.55 ± 0.34 µg/mL and 15.37 ± 0.17 µg/mL against L. major and L. infantum, respectively. The potentialities of antibacterial and leishmanicidal activities found in dichloromethane could be explained by the presence of major flavonoids such as catechin, rutin and luteolin 7-O-glucoside as revealed by HPLC system. The observed moderate antifungal activity, which was only given by butanolic fraction against pathogen fungi, may be attributed to the presence of chlorogenic acid. Furthermore, dichloromethane and butanolic fraction showed a good DPPH (2,2-diphenyl-1-picryl hydrazyl) scavenging activity and Ferric reducing power. These results suggest that Ziziphus lotus leaf fractions might be used as antioxidant and antimicrobialagent.
Asunto(s)
Antiinfecciosos , Staphylococcus aureus Resistente a Meticilina , Ziziphus , Antibacterianos/farmacología , Antiinfecciosos/farmacología , Antioxidantes/farmacología , Pruebas de Sensibilidad Microbiana , Extractos Vegetales/farmacología , Hojas de la Planta , Staphylococcus aureusRESUMEN
The preparation, chemical properties and bio-activities of polysaccharides derived from halophytes have gained an increasing interest in the past few years. Phytochemical and pharmacological reports have shown that carbohydrates are important biologically active compounds of halophytes with numerous biological potentials. It is believed that the mechanisms involved in these bio-activities are due to the modulation of immune system. The main objective of this summary is to appraise available literature of a comparative study on the extraction, structural characterizations and biological potentials, particularly immunomodulatory effects, of carbohydrates isolated from halophytes (10 families). This review also attempts to discuss on bioactivities of polysaccharides related with their structure-activity relationship. Data indicated that the highest polysaccharides yield of around 35% was obtained under microwave irradiation. Structurally, results revealed that the most of extracted carbohydrates are pectic polysaccharides which mainly composed of arabinose (from 0.9 to 72%), accompanied by other monosaccharides (galactose, glucose, rhamnose, mannose and xylose), significant amounts of uronic acids (from 18.9 to 90.1%) and some proportions of fucose (from 0.2 to 8.3%). The molecular mass of these pectic polysaccharides was varied from 10 to 2650 kDa. Hence, the evaluation of these polysaccharides offers a great opportunity to discover novel therapeutic agents that presented especially beneficial immunomodulatory properties. Moreover, reports indicated that uronic acids, molecular weights, as well as the presence of sulfate and unmethylated acidic groups may play a significant role in biological activities of carbohydrates from halophyte species.
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Pectinas/química , Pectinas/farmacología , Plantas Tolerantes a la Sal/química , Secuencia de Carbohidratos , Inmunomodulación , Microondas , Pectinas/aislamiento & purificación , Relación Estructura-ActividadRESUMEN
Alginate nanocomposite films incorporating sepiolite (Sep) modified with myrtle berries extract (MBE) rich in polyphenols were prepared by solution casting method. The effects of different extract concentrations on the film properties were determined by measuring physicochemical, mechanical and antioxidant properties of the films. Fourier transform infrared (FTIR) spectra indicated that strong interactions between the polyphenols present in the MBE and sepiolite were involved in the films. The results suggested that incorporation of Sep-MBE hybrids into the films improved elongation at break, tensile strength, water vapor and UV barrier properties compared to the control film. The antioxidant activity of the films was significantly improved and raised with increasing content of MBE. The release kinetics results of MBE polyphenols from the active films into alcoholic food simulant indicated that the addition of Sep-MBE hybrids to alginate film is able to slow the release of MBE polyphenols. This study revealed the benefits of incorporation of Sep-MBE hybrids into the alginate films and their potential application as active packaging films or coating material.
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Alginatos/química , Frutas/química , Silicatos de Magnesio/química , Myrtus/química , Nanocompuestos/química , Extractos Vegetales/química , Polifenoles/química , Adsorción , Antioxidantes/farmacología , Rastreo Diferencial de Calorimetría , Módulo de Elasticidad , Humedad , Nanocompuestos/ultraestructura , Fenómenos Ópticos , Permeabilidad , Polifenoles/farmacología , Espectroscopía Infrarroja por Transformada de Fourier , Vapor/análisis , Temperatura , Resistencia a la Tracción , Termogravimetría , Agua/químicaRESUMEN
The aim of this work was to perform an unprecedented in-depth study on the bioactive phytochemicals of Abelmoschus esculentus L. Moench Tunisian landrace (Marsaouia). For this purpose, its nutritional, aroma volatile, and phenolic profiles were characterized, and sundry biological activities were assessed in vitro. The approximate composition revealed that total dietary fiber as the most abundant macronutrient, mainly insoluble dietary fiber, followed by total carbohydrates and proteins. In addition, okra pods were rich in K, Ca, Mg, organic acids, tocopherols, and chlorophylls. Gas Chromatography-Electron Impact Mass Spectrometry (GC-EIMS) analysis showed that oxygenated monoterpenes, sesquiterpene hydrocarbons, and phenylpropanoids were the predominant essential volatile components in A. esculentus pods. A total of eight flavonols were detected by High-Performance Liquid Chromatography coupled to a DAD detector and mass spectrometry by electrospray ionization (HPLC-DAD-MS/ESI); with quercetin-3-O-glucoside being the majority phenolic component, followed by quercetin-O-pentosyl-hexoside and quercetin-dihexoside. This pioneering study, evidences that Tunisian okra display promising antioxidant and cytotoxic actions, in addition to relevant inhibitory effects against α-amylase and α-glucosidase enzymes, and interesting analgesic activity.
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Abelmoschus/química , Antioxidantes/química , Evaluación Nutricional , Fenoles/química , Fibras de la Dieta/análisis , Glucósidos/química , Humanos , Monoterpenos/química , Valor Nutritivo , Extractos Vegetales/química , Quercetina/análogos & derivados , Quercetina/química , Sesquiterpenos/químicaRESUMEN
A Box-Behnken design was used to optimize extraction temperature, extraction time and concentration of the salicylic acid to obtain a maximum polysaccharide yield from Citrus sinensis peels. The optimal settings were: extraction time 3 h, extraction temperature 80 °C and concentration of the salicylic acid 1.5%. Under these conditions, the experimental yield and uronic acid content were 11.74% and 66.9% respectively. Preliminary characterization was performed via FT-IR, SEC/MALS/VD/DRI and GC-MS after hydrolysis. SEC analysis showed that the extracted polysaccharide had a weight average molar mass of 350 kDa and an intrinsic viscosity of 640 mL/g. The GC-MS results revealed that the extracted polysaccharide was composed of arabinose 56.7%, galactose 17.8%, xylose 13.8%, rhamnose 5.1%, mannose 2.5% and glucose 1.5% suggested a rhamnogalacturonan pectin type I with a degree of esterification of 50.9% (IRTF). The flow curve and the dynamic frequency sweep were obtained at 10, 20, 30 and 40 g/L in water and at 30 g/L in presence of CaCl2 or NaCl at 1 mol/L. The solutions showed shear-thinning behavior fitted with Ostwald-De Waele model, except 10 g/L with a Newtonian behavior. The apparent viscosity and, the G' and G" moduli increase with PACO concentration in agreement with a slow-down of the dynamic chain. In the presence of CaCl2 or NaCl the reduction of electrostatic repulsions between pectin chains decreases the rheological parameters. The effect is less sensitive with CaCl2 due to intermolecular interactions. The antiproliferative activity of the extracted pectin on human Caco-2 and Hep-2 cells was very interesting with an IC50 1.4 and 1.8 µg/mL respectively.
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Antineoplásicos Fitogénicos/química , Antineoplásicos Fitogénicos/farmacología , Citrus sinensis/química , Pectinas/química , Pectinas/farmacología , Reología , Ácido Salicílico/química , Antineoplásicos Fitogénicos/aislamiento & purificación , Carbohidratos/química , Carbohidratos/aislamiento & purificación , Línea Celular Tumoral , Proliferación Celular/efectos de los fármacos , Cromatografía en Gel , Humanos , Peso Molecular , Pectinas/aislamiento & purificación , Proteínas de Plantas/química , Proteínas de Plantas/aislamiento & purificación , Polisacáridos/química , Polisacáridos/aislamiento & purificación , Ácido Salicílico/aislamiento & purificación , Solubilidad , Espectroscopía Infrarroja por Transformada de FourierRESUMEN
The use of biological immunostimulants is considered a valuable practice to improve culture conditions in aquaculture sector that may help to increase production and maintain healthy environment. We undertook this study in order to evaluate the potential effect of the administration of two exopolysaccharides (EPS) "EPLB" and "EPB" derived from potential probiotic strains on immune and antioxidant status of European sea bass (Dicentrarchus labrax L.) larvae. In order to find out if the EPSs have an effect on the biochemical composition during the trial period, the nutritional value has been evaluated. The results revealed that expression levels of immune-relevant genes (infg, Il1b, Il8, Il6 and tcr-ß) in the gut and head kidney and the scavenging enzymes (cat, sod, gr) genes in the liver were modulated. In fact, the dietary supplementation with the tested EPSs, significantly enhances the expression of immune-associated genes in the head-kidney, particularly infg and tcrß, as well as catalase gene in liver. During the period of study, EPSs administration did not affect the fatty acid profiles of larvae, which is balanced. This is confirmed by the Docosahexaenoic acid / Eicosapentaenoic acid ratio and demonstrates that EPLB and EPB can be administrated without any negative effect on biochemical composition of European sea bass. The present findings provided evidence that the tested EPSs with antibacterial and antioxidant activities can enhance immune response without negative effect on the biochemical composition. The used EPSs can be considered as a good source of natural functional aquafeed ingredients for European sea bass.
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Lubina , Polisacáridos Bacterianos/farmacología , Probióticos , Alimentación Animal/análisis , Fenómenos Fisiológicos Nutricionales de los Animales , Animales , Antioxidantes/metabolismo , Acuicultura , Dieta/veterinaria , Ácidos Grasos/metabolismo , Valor NutritivoRESUMEN
The cadmium (Cd) is considered one of the widespread toxic metals in the aquatic and terrestrial environments, which is due to its long half-life, non-degradable characteristic, and toxicity. Aqueous extract of freeze-dried Moringa oleifera (Moringaceae family) leaves was examined for protective effect and antioxidant power against Cd toxicity. The results revealed that Moringa aqueous extract (MAE) has contents of total polyphenols and flavonoids about 30.14 mg GAE/g and 18.35 mg QE/g respectively. Furthermore, phenolic compounds in leaves of Moringa were studied using a high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS). Results showed that the largest number of phenolic compounds determined in leaves of Moringa belongs to flavonoids. Moreover, biological properties were determined by radical scavenging capacity (DPPH) and ferric-reducing power (FRAP). Cytoprotective effect and antioxidant power of Moringa extract were assessed using the mitochondrial activity testing method (MTT test), malondialdehyde (MDA), and reactive oxygen species (ROS) production. Results indicate that Moringa aqueous extract have a significant (i) proliferative, (ii) antioxidant, and (iii) cytoprotective effect on HCT116 and HEK293 cells against metal toxicity.
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Moringa oleifera , Antioxidantes , Cadmio , Células HEK293 , Humanos , Extractos Vegetales , Hojas de la PlantaRESUMEN
In this study, we investigated a novel aflatoxin biosensor based on acetylcholinesterase (AChE) inhibition by aflatoxin B1 (AFB1) and developed electrochemical biosensors based on a sodium alginate biopolymer as a new matrix for acetylcholinesterase immobilization. Electrochemical impedance spectroscopy was performed as a convenient transduction method to evaluate the AChE activity through the oxidation of the metabolic product, thiocholine. Satisfactory analytical performances in terms of high sensitivity, good repeatability, and long-term storage stability were obtained with a linear dynamic range from 0.1 to 100 ng/mL and a low detection limit of 0.1 ng/mL, which is below the recommended level of AFB1 (2 µg/L). The suitability of the proposed method was evaluated using the samples of rice supplemented with AFB1 (0.5 ng/mL). The selectivity of the AChE-biosensor for aflatoxins relative to other sets of toxic substances (OTA, AFM 1) was also investigated.
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Acetilcolinesterasa/química , Aflatoxina B1/análisis , Alginatos/química , Técnicas Biosensibles , Inhibidores de la Colinesterasa/análisis , Acetiltiocolina/química , Aflatoxina B1/química , Inhibidores de la Colinesterasa/química , Espectroscopía Dieléctrica , Contaminación de Alimentos/análisis , Oryza/químicaRESUMEN
In this study, Box-Behnken Design was used to optimize the ultrasonic extraction of polysaccharides from quince peels (QPPs) by ascorbic acid and the effect of extraction temperature, extraction time and pH was evaluated. Under optimized conditions of temperature 90 °C, 60 min sonication time and pH = 3.26, the extraction yield, the galacturonic acid yield and the concentration of sample required to scavenge 50% of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic) acid (ABTS) values of QPPs were respectively 10.25%, 3.86% and 1.35 mg/mL. The QPPs extracted under optimum conditions was characterized by Fourier transform infrared spectroscopy (FTIR), Nuclear magnetic resonance (1 H NMR) and Size exclusion chromatography (SEC/MALS/VD/DRI). The monosaccharide analysis revealed that arabinose was the most abundant, followed by galactose, glucose, mannose and xylose. Moreover, QPPs showed significant antioxidant activities (2,2-diphenyl-1-picrylhydrazyl (DPPH) and Ferric- reducing antioxidant power (FRAP)) and reduced viability of human Caco-2 and murine B-16 cell lines in a dose-dependent manner. Hence QPPs could be used as antitumor agent in functional foods andpharmaceutical industries.
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Antineoplásicos Fitogénicos/farmacología , Antioxidantes/farmacología , Polisacáridos/aislamiento & purificación , Polisacáridos/farmacología , Rosaceae/química , Animales , Antineoplásicos Fitogénicos/administración & dosificación , Antioxidantes/química , Células CACO-2 , Supervivencia Celular/efectos de los fármacos , Fraccionamiento Químico , Cromatografía en Gel , Relación Dosis-Respuesta a Droga , Frutas/química , Ácidos Hexurónicos/aislamiento & purificación , Humanos , Concentración de Iones de Hidrógeno , Espectroscopía de Resonancia Magnética , Ratones , Monosacáridos/análisis , Monosacáridos/química , Extractos Vegetales/química , Polisacáridos/química , Espectroscopía Infrarroja por Transformada de Fourier , TemperaturaRESUMEN
OBJECTIVE: Age-related diseases, including neurodegenerative diseases, are associated with oxidative stress and lipid peroxidation, and increase the levels of cholesterol auto-oxidation products such as 7ß-hydroxycholesterol (7ß-OHC). Thus, it is imperative to identify agents that can prevent 7ß-OHC-induced side-effects. METHODS: We evaluated the potential protective effects of Carpobrotus edulis ethanol-water extract (EWe) on murine oligodendrocytes (158N) cultured in the absence or presence of 7ß-OHC (20 µg/mL, 24 h). The cells were incubated with EWe (20-200 µg/mL) 2 h before 7ß-OHC treatment. Mitochondrial activity and cell growth were evaluated with the MTT assay. Photometric methods were used to analyze antioxidant enzyme [catalase (CAT) and glutathione peroxidase (GPx)] activities and the generation of lipid and protein oxidation products [malondialdehyde (MDA), conjugated diene (CD), and carbonylated proteins (CPs)]. RESULTS: Treatment with 7ß-OHC induced cell death and oxidative stress (reflected by alteration in CAT and SOD activities). Overproduction of lipid peroxidation products (MDA and CDs) and CPs was also reported. The cytotoxic effects associated with 7ß-OHC were attenuated by 160 µg/mL of EWe of C. edulis. Cell death induced by 7ß-OHC treatment was ameliorated, GPx and CAT activities were restored to normal, and MDA, CD, and CP levels were reduced following C. edulis extract treatment. CONCLUSION: These data demonstrate the protective activities of C. edulis EWe against 7ß-OHC-induced disequilibrium in the redox status of 158N cells, indicative of the potential role of this plant extract in the prevention of neurodegenerative diseases.
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Aizoaceae , Enfermedades Neurodegenerativas/prevención & control , Oligodendroglía/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Extractos Vegetales/farmacología , Animales , Línea Celular , Evaluación Preclínica de Medicamentos , Hidroxicolesteroles , Ratones , Neuroprotección , Oligodendroglía/metabolismo , Fitoterapia , Extractos Vegetales/uso terapéuticoRESUMEN
Spinacia is an interesting medicinal halophyte plant that is employed as a food and therapeutic agent in traditional medicine. In this work, water-soluble polysaccharides from Spinacia oleracea were extracted and preliminary characterization was established via FT-IR, UV-vis, 1H NMR and SEC/MALS DRI technics. The extracted polysaccharide, with an average molecular mass of 408â¯kDa, was composed of arabinose, galactose, mannose, glucose, rhamnose and xylose in the molar percentage of 49.3%, 28.1%, 4.9%, 7.8%, 8.2% and 1.7%, respectively. The polysaccharide showed significant antioxidant activity. Moreover, Spinacia polysaccharide, significantly prevented oxidation-induced Cd damage and exhibited a protective effect against Cd cytotoxicity on HEK293 and HCT116 cells, with an important cell viability decrease, an important reduction of MDA production and ROS levels. The outcomes obtained suggest that the Spinacia polysaccharides may be used as an accessible source of natural antioxidants and as potential phytochemicals against kidney and colon cancer.
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Antioxidantes/química , Antioxidantes/farmacología , Cadmio/toxicidad , Citoprotección/efectos de los fármacos , Polisacáridos/química , Polisacáridos/farmacología , Spinacia oleracea/química , Benzotiazoles/química , Compuestos de Bifenilo/química , Supervivencia Celular/efectos de los fármacos , Células HCT116 , Células HEK293 , Humanos , Peroxidación de Lípido/efectos de los fármacos , Picratos/química , Especies Reactivas de Oxígeno/metabolismo , Ácidos Sulfónicos/químicaRESUMEN
Nutritional, soluble carbohydrates and aroma volatile profiles of Tunisian wild Swiss chard leaves (Beta vulgaris L. var. cicla) have been characterized. The chemical composition of an ethanol chard leaves extract, as well as its in vitro antioxidant, α-amylase and α-glucosidase inhibition activities were carefully evaluated. The results of the proximate composition showed that total carbohydrate fraction, mainly as dietary fiber were the major macronutrient (2.43â¯g/100â¯g fw), being the insoluble dietary fiber the predominant fraction (2.30â¯g/100â¯g). In addition, leaves of Beta vulgaris L. were especially rich in Mg, Fe and Ca (4.54, 2.94 and 2.28â¯mg/100â¯g fw) and very poor in Na (0.09â¯mg/100â¯g fw). Volatile profile revealed that non-terpene derivatives and sesquiterpene hydrocarbons were the essential classes of volatiles in the chard leaves. Myricitrin, p-cumaric acid and rosmarinic acid were characterized. Moreover, the ethanol extract of wild Swiss chard leaves revealed significant antioxidant capacity. Furthermore, a good enzyme inhibitory effects on α-glucosidase and α-amylase activities were observed. These findings highlighted the potential health benefits of wild Swiss Chard as a source of nutritional and bioactive compounds.
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Beta vulgaris/química , Fitoquímicos/análisis , Extractos Vegetales/análisis , Hojas de la Planta/química , Antioxidantes/análisis , Carbohidratos/análisis , Fibras de la Dieta , Inhibidores Enzimáticos/análisis , Ácidos Grasos/análisis , Flavonoides/análisis , Hidroxibenzoatos/análisis , Hidroxibenzoatos/aislamiento & purificación , Minerales/análisis , Valor Nutritivo , Pigmentos Biológicos , Terpenos , Compuestos Orgánicos Volátiles/análisis , Compuestos Orgánicos Volátiles/aislamiento & purificaciónRESUMEN
In order to develop new and low cost anticoagulants as potential heparin alternatives, sulfation of a pectin-like polysaccharide from Opuntia ficus indica cladodes using SO3-DMF complex was performed with a significant sulfate content (7%). FTIR and NMR assays indicated that the sulfation reaction had occurred. In addition, GC-MS analyses demonstrated that sulfation was carried out on the arabinose units of native polysaccharide. Moreover, Physico-chemical characterization indicated an evident decrease of the average molecular weight (Mw) and sugar rates after sulfation. Finally, anticoagulant assays demonstrated that the anticoagulant activity was significantly enhanced by the addition of sulfate groups. Thus, sulfated polysaccharides exhibited the most potent anticoagulant activity by prolonging activated partial thromboplastin time (APTT) and thrombin time (TT).
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Anticoagulantes/química , Anticoagulantes/farmacología , Opuntia/química , Pectinas/química , Pectinas/farmacología , Sulfatos/química , Tiempo de TrombinaRESUMEN
Prunus amygdalus is used in the folk medicine that proved the interest of this plant which cures many diseases. Many researchers are interested to valorize almond waste (hull and shell) and to evaluate their biological and pharmacological activities. In this work, polysaccharides from Prunus amygdalus shell were extracted sequentially by water, ammonium oxalate and hydrochloric acid. The monosaccharide composition of polysaccharides fractions was performed by GC-MS. Water-soluble polysaccharide was found to be the most effective extracting agent with an extracted yield of 9%. The acid Soluble Polysaccharides (ASP) exhibited the highest galacturonic acid content (31.95%), the highest polysaccharides extractability (88.57%) and the lowest degree of esterification (31.76%). The different polysaccharides fractions were characterized by FTIR, 1H NMR and SEC/MALS/VD/DRI. The antioxidant tests (DPPH, ABTS and FRAP) indicated that ASP showed even better antioxidant activities. Moreover, the result of the antiproliferative activity against Caco-2 and B-16 cells showed that ASP exhibited strong cytotoxicity ability which confirmed that the Prunus amygdalus peels may comprise the natural raw materials for new drug and functional food.
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Antineoplásicos/farmacología , Antioxidantes/farmacología , Extractos Vegetales/farmacología , Polisacáridos/farmacología , Prunus dulcis/química , Antineoplásicos/química , Antioxidantes/química , Fenómenos Químicos , Cromatografía de Gases y Espectrometría de Masas , Ácidos Hexurónicos/química , Espectroscopía de Resonancia Magnética , Fenol/química , Extractos Vegetales/química , Polisacáridos/química , Solubilidad , Espectroscopía Infrarroja por Transformada de FourierRESUMEN
Box-Behnken design (BBD) was performed via ultrasound-assisted system to optimize extraction yield and total antioxidant ability of polysaccharides from Suaeda fruticosa. Three independent variables were studied: temperature, time and pH with optimal settings 90⯰C, 37â¯min and 2.9, respectively. Under these settings, isolation yield and total antioxidant activity were 34.0% and 31.8â¯mg ascorbic acid equivalent/g material, respectively. Furthermore, preliminary structure study was carried out using UV-vis analysis, Fourier transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC)) methods Size exclusion chromatography (SEC) determination revealed that polysaccharide extracted had an average molecular weight of 240â¯kDa. Chemical composition analyses showed that extracted polysaccharide was a pectin-like polysaccharide with uronic acid content (47.5%) and neutral monosaccharides (52.5%) constituted mainly of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively. This sugar composition was typical of that of a galactoarabinan. Importantly, the optimum polysaccharide possesses significant potentials in antioxidant activity effect. Moreover, pharmacological investigation of extracted polysaccharide revealed a good anti-inflammatory effect and interesting peripheral as well as central antinociceptive activities.
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Analgésicos/química , Antiinflamatorios/química , Antioxidantes/química , Chenopodiaceae/química , Pectinas/química , Analgésicos/uso terapéutico , Animales , Antiinflamatorios/uso terapéutico , Antioxidantes/uso terapéutico , Edema/tratamiento farmacológico , Miembro Posterior/lesiones , Ratones , Monosacáridos/análisis , Pectinas/uso terapéutico , Hojas de la Planta/química , Ratas , Ratas WistarRESUMEN
The aim of this study is to isolate pectin from peel (WNPE) and pulp (WNPU) of Opuntia microdasys var. rufida's (OMR) cladodes and to characterize these polysaccharides by size exclusion (SEC/MALS/VD/DRI), gas chromatography coupled to mass spectrometer (GC-MS), nuclear magnetic resonance (1H NMR), and Fourier transform infrared spectroscopy (FTIR) analysis. The polysaccharides were extracted in neutral aqueous media followed by ethanol precipitation and dialysis. Both WNPE and WNPU are mainly composed of uronic acids and some neutral sugars such as arabinose, galactose, rhamnose and mannose. Their molecular weight range from 2,180,000 and 4,920,000g/mol. The in-vivo pharmacological activities (anti-inflammatory, analgesic and gastroprotective activities) have been performed. The extracted pectin (50-100mg/kg, i.p. (intraperitoneal)) inhibited, in a dose-related manner, both carrageenan-induced paw edema in rats and Xylene-induced ear edema in mice. A dose-dependent action was obtained against chemical (writhing test) and thermic (hot plate test) stimuli, respectively, with doses of 50 and 100mg/kg. Moreover, a considerable gastroprotective effect was observed with these two biopolymers, the gastric ulcer was attenuated until 67.67% for WNPE and 81.93% for WNPU, at the dose of 100mg/kg.
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Edema/tratamiento farmacológico , Opuntia/química , Dolor/tratamiento farmacológico , Pectinas/administración & dosificación , Polisacáridos/química , Animales , Carragenina/toxicidad , Cromatografía de Gases , Edema/inducido químicamente , Edema/patología , Humanos , Espectroscopía de Resonancia Magnética , Espectrometría de Masas , Ratones , Dolor/inducido químicamente , Dolor/patología , Pectinas/química , Polisacáridos/administración & dosificación , Ratas , Espectroscopía Infrarroja por Transformada de Fourier , Xilenos/toxicidadRESUMEN
In this study, Box-Behnken design was used to optimize the ultrasonic extraction of Carpobrotus edulis polysaccharides (CEP), and the effect of time, extraction temperature and water to material ratio was evaluated. Optimum conditions were 1.77h, 78.0°C and 33.04mL/g to improved CEP yield (7.84%), which is in good agreement with the predicted yield 7.77%. Then, the physico-chemical, antioxidant and antiglycation properties of optimized CEP were studied, and the total sugar and galacturonic acid content were 89.7 and 63.2%, respectively. The composition of neutral monosaccharide was arabinose, xylose, rhamnose and mannose in the molar percentage of 71.84, 14.80, 8.57, and 4.79%, respectively. In addition, (1H, and 13C) NMR and FTIR analyses confirmed the presence of uronic acids in the free and methyl ester forms with a degree of esterification of 31.27%. Therefore, this finding showed that CEP is a low methoxyl pectic polysaccharide, with an average molecular weight about 65,000g/mol. Finally, the results indicated that CEP presents strong antioxidant activities in vitro (DPPH, chelating ability and reducing power), and significantly inhibits lipid peroxidation and the formation of fluorescent advanced glycation end products in glucose-BSA system model.
Asunto(s)
Aizoaceae/química , Antioxidantes/química , Extractos Vegetales/química , Polisacáridos/química , Antioxidantes/farmacología , Depuradores de Radicales Libres , Glucosa/química , Ácidos Hexurónicos/química , Hipoglucemiantes/química , Hipoglucemiantes/farmacología , Peroxidación de Lípido/efectos de los fármacos , Espectroscopía de Resonancia Magnética , Monosacáridos/química , Extractos Vegetales/farmacología , Polisacáridos/aislamiento & purificación , Polisacáridos/farmacología , Albúmina Sérica Bovina/química , Espectroscopía Infrarroja por Transformada de Fourier , Azúcares/química , Ácidos Urónicos/química , Agua/químicaRESUMEN
In the food industry, there is a continuous search for ingredients that might provide advantageous properties to food products, either considering their nutritional value or bioactivity, as well as flavouring and technological aspects. Crude oils are good examples of this type of ingredient, especially if obtained from nonconventional sources. Accordingly, the Opuntia macrorhiza Engelm. seed oil (OMSO) was chemically characterized and evaluated for different in vitro and in vivo bioactivities. OMSO presented physicochemical characteristics appropriate to be considered as an edible oil, namely low acidity value, stability to oxidation (high peroxide value and low K232 and K270 values), and high contents of unsaturated fatty acids (as shown by the iodine value) and saponifiable matter. Furthermore, this natural oil, owing to its rich phytochemical profile, showed relevant antioxidant activity (especially in lipid peroxidation inhibition assays), α-glucosidase inhibitory activity, cytotoxicity against human tumour cell lines, antibacterial (mainly against Gram positive species) and antifungal properties, as well as anti-inflammatory and analgesic activities. Furthermore, OMSO did not show any sign of acute toxicity on animals, highlighting its possible use in different applications, considering that this natural product is not expected to induce the adverse effects typically associated with synthetic bioactive agents (e.g., ampicillin, amphotericin B, or lysine acetylsalicilate).