Retention and Separation Changes of Ternary and Quaternary Alkaloids from Chelidonium majus L. by TLC Under the Influence of External Magnetic Field.

Thin layer techniques proved their usefulness in the analysis of plant extract material. Thanks to their low operation costs, high sample throughput and possibility to gather chromatographic data for the whole sample in a single act, they allowed to find, and in some cases identify, active ingredients often hidden in sophisticated plant extract matrices. It was also proved that the presence of a magnetic field changes the retention of some substances, and moreover changes the separation efficiency of chromatographic systems. In the presented experiment, retention, efficiency and separation abilities of TLC chromatographic systems for mixtures of alkaloids under the influence of magnetic field depending on inductivity of the magnetic field, the saturation of chromatographic chamber and quantity of chromatographed substances were investigated. The results obtained were tested on real plant extracts that revealed the ability of chromatography in a magnetic field for separation of ternary and quaternary alkaloids from Chelidonium majus L. Our experiments proved that the presence of magnetic field induction lines perpendicular to the chromatographic plate plane can influence the width and retention of chromatographed substances, and can be considered as a tool for separation adjustment of plant extracts containing ternary and quaternary alkaloids.

Skin-mimetic chromatography for prediction of human percutaneous absorption of biologically active compounds occurring in medicinal plant extracts.

The main aim of this study was to predict quantitatively human percutaneous absorption of chosen compounds commonly occurring in plants which can be used as medicinal extracts in the drug and beauty industries. The most important human percutaneous descriptors, i.e. logKp (logarithm of the water/skin partition coefficient) and logJmax (logarithm of the maximum flux of solutes penetrating the skin), of fatty acids and polyphenols were determined using both in vitro and in silico methods. For in vitro determination of human percutaneous absorption, micellar liquid chromatography based on hexadecyltrimethylammonium bromide, sodium dodecyl sulfate and polyoxyethylene (23) lauryl ether (Brij35) was used. Human percutaneous absorption was characterized by entirely new QSAR/QRAR models based on retention, lipophilic, steric and electronic data as well as on the linear free energy relationship parameters. Many different correlations between human skin absorption and different physicochemical parameters were performed, e.g. the in silico estimated logKp value was correlated with the retention parameter logkw (logarithm of the retention factor extrapolated to pure water) from the systems imitating a cutaneous environment (R2 = 0.92). Moreover, the influence of lipophilicity on percutaneous absorption was examined. The obtained correlation was excellent (R2 = 0.95).

[Successes and failures of ozena's medical treatment]. / Sukcesy i porazki zachowawczego leczenia ozeny.

Ozena belongs to the primary atrophic rhinitis of unknow etiology. The incidence of this disease has markedly decrease in the last decades. We treated 4 patients with ozena between 2000-2005. The diagnosis was confirmed by physical and ENT examination, culture from the nose and CT scan of perinasal sinusis. Ciprofloxacin was aministrated orally in a dose 1,0-0,5 g daily to all patients. Also they were instructed how to clean the nose regularly with an isotonic saline solution and to moisteurse nosa by wotery spray. The treatment with ciprofloxacin was maintained for 4-6 weeks. In 3 causes the result was very good after 2-4 y observation. 1 patient, 16 y. old girl, didn't cooperate therapy propely - she stoped nasal rinsing after some improvement. The second course of treatment seems to get good result (3 months observation). Our and some other authors conclusion is: ciprofloxacin seems to be a promising drug for the treatment of ozena. For good results cleanising the nose regularly is importent as well as antibiotic.