Screening of Phyllanthus niruri Leaves Phytoconstituents for Antiviral and Antibacterial Activity by Molecular Docking Studies.

The genus Phyllanthus belongs to one of the largest plant families, the Phyllantaceae (L.). Phyllanthus niruri is an annual perennial herb that grows in tropical Asia, America, China, and the islands of the Indian Ocean. Numerous alkaloids, steroids, flavonoids, lignans, coumarins, polyphenols, and lipids are present in Phyllanthus. The effects of plants have been studied for a variety of purposes, including their antioxidant (Giribabu et al., Evid Based Complement Alternat Med, 2014), anti-inflammatory (Porto et al., Revista Brasileira de Pharmacognosy, 2013), antinociceptive (Sathisha et al., Indian Drugs, 2009), analgesic (Mostofa et al., BMC Complement Altern Med, 2017), antiulcer (Mali et al., Biomed Aging Pathol, 2011), antiarthritic (Obidike and Salawu, Planta Medica, 2010), antiplasmodial (Shilpa et al., Environ Dis, 2018), immunomodulatory (Manikkoth et al., Anticonvulsant activity of Phyllanthus amarus in experimental animal models), anticonvulsant (Wasnik et al., Int J Pharm Sci Rev Res, 2014), antidepressant (Venkateswaran et al., Effects of an extract from Phyllanthus niruri on hepatitis B and woodchuck hepatitis viruses: In vitro and in vivo studies (antiviral agent/Marmota monax/DNA polymerase/hepatitis B surface antigen/woodchuck hepatitis surface antigen). In Hepatitis B and The Prevention of Primary Cancer of The Liver: Selected Publications of Baruch S Blumberg, pp 535-539), antiviral (Venkateswaran et al., Effects of an extract from Phyllanthus niruri on hepatitis B and woodchuck hepatitis viruses: In vitro and in vivo studies (antiviral agent/Marmota monax/DNA polymerase/hepatitis B surface antigen/woodchuck hepatitis surface antigen). In Hepatitis B and The Prevention of Primary Cancer of The Liver: Selected Publications of Baruch S Blumberg, pp 535-539), antitumor (Sharma et al., Asian Pac J Cancer Prev, 2009), hyperlipidemia (Khanna et al., J Ethnopharmacol, 2002), and antifertility (Ezeonwu, Inquiries J, 2011). For additional docking investigations with distinct proteins, the leaf chemicals are assessed, that is, the crystal structure of serine protease hepsin in complex with inhibitor [PDB ID:5 CE1] for antiviral activity human topoisomerase II beta in complex with DNA and etoposide [PDB ID:3QX3] and crystal structure of E. coli GyraseB 24 kDa in complex with 4-(4-bromo-1H-pyrazol-1-yl)-6-[(ethylcarbamoyl)amino]-N-(pyridin-3-yl) pyridine-3-carboxamide [PDB ID: 6F86] for antibacterial activity and have been selected. To evaluate the in silico results and grading of virtual screening, or molecular docking, ritonavir antiviral activity and ampicillin for antibacterial activity were used as a benchmark.

Screening of Phyllanthus niruri Plant Active Constituents for Anticancer and Antifungal Activity by Insilico Methods.

A large genus of shrubs, trees, and rare plants belonging to the Euphorbiaceae family, Phyllanthus contains 600-700 species. The Phyllanthus niruri (L.) species is a tiny, erect annual herb that can reach heights of 30-40 cm. Its 7-12 cm long, sessile, alternating leaves are native to the Amazon rainforest, but they can also be found in other tropical regions such as South East Asia, Southern India, America, China, and the islands of the Indian Ocean. Phyllanthus contains many classes of alkaloids, steroids, flavonoids, lignin, polyphenols, and lipids. Numerous activities of the plant have been studied, including antidepressant (Wasnik et al., Int J Pharm Sci Rev Res, 6:26-29, 2014), anticancer (Sayuti et al. Studies, 10:17, 2020), anti-inflammatory, antinociceptive (Porto et al., Revista Brasileira de Farmacognosia, 23:138-144, 2013), analgesic (Bhat et al., Pharm Res, 7:378, 2015), antiarthritic (Mali et al., Biomed Aging Pathol, 1:185-190, 2011), immunomodulatory, antibacterial, antifungal (Shilpa et al., Environm Dis, 3:63, 2018), antidiabetic (Kumar et al., Biomed Pharm J, 12:57-63, 2019), antiulcer (Mostofa et al., BMC Complement Altern Med, 17:1-10, 2017), antiviral (Wahyuni et al., Malays Appl Biol, 48:105-111, 2019), antiplasmodial (Ifeoma et al., Asian Pacific J Trop Med, 6:169-175, 2013), anticonvulsant (Amaechina and Omogbai, Nig J Nat Prod Med, 17:61-65, 2013), and hepato human cytochrome P450 CYP17A1 in association with abiraterone [PDB ID: 3RUK] plant extracts. New selective androgen receptor modulators were synthesized, and they were biologically evaluated (SARMs) (Micah et al., J Veter Med Anim Health 5(1):8-15, 2013, Rusmana et al., Indonesian Biomed J 9(2):84-90, 2017, Al Zarzour et al., Nutrients 10(8):1057, 2018, Khanna J Ethnopharmacol 82(1):19-22, 2002). In the present study [PDB ID: 3RUK,5T8E] with anticancer and [PDB ID: 6F0E,1EA1] with antifungal activities were used for docking study. In this study fluconazole's antifungal activity and dacarbazine's anticancer activity were used as benchmarks for molecular docking with Schrodinger 13.0 to compare the activity of Phyllanthus niruri's active constituents.