Flaxseed gum based biocomposite film modified with betel leaf extract: A novel packaging material for oxidative stability of meat patties.

The study investigated the antioxidant effect on lipid and protein oxidation, microbial count and other physicochemical attributes of meat patties packaged in flaxseed gum (FSG) based films added with betel leaf extract (BLE) during refrigerated storage (4 ± 1 °C) of 30 days. FSG films were developed after incorporating 0, 2.5, 5, 7.5 and 10% of BLE (BLE0, BLE1, BLE2, BLE3 and BLE4) respectively. The patties showed no change in pH due to composite films however, a remarkable effect in retarding the weight loss and color change along with an improvement in sensory score and microbial quality. TBARS of the patties packed in treated films ranged from 0.10 to 0.99 (mg MDA/kg), lower than that of the control 0.34-1.33 (mg MDA/kg). The BLE4 (packed in FSG film with 10% BLE) had the lowest metmyoglobin content of 31.71% compared to the control sample (69.02%) on 30th day of refrigerated storage. Further, a significant reduction in moisture and color change was observed in meat patties packed in FSG-BLE composite films compared to the control patties. Hence, this study concluded that the FSG-BLE composite films improves the storage stability by impeding the rate of lipid oxidation indicating the developed film's promising potential as a sustainable material in active packaging for the shelf life extension of high-fat meat products and other perishable food products.

Development and characterization of edible films based on flaxseed gum incorporated with Piper betle extract.

There has been a shift from use of petroleum-based plastics, causing serious environmental pollution, towards innovative and biodegradable edible packaging. The present study documents the development of composite edible films based on the flaxseed gum (FSG) modified by the incorporation of betel leaf extract (BLE). The films were assessed for physicochemical, mechanical, morphological, thermal, antimicrobial and structural characteristics. Scanning electron microscopy images indicated that the roughness decreased with an increase in BLE concentration. The water vapor permeability of the FSG-BLE films ranged from 4.68 to 1.59 × 10-9 g s- 1 m- 2 Pa- 1, lower than that of the control sample (6.77 × 10-9 g s- 1 m- 2 Pa- 1). The BLE4 (containing 10 % BLE) films had the highest tensile strength of 32.46 MPa compared to the control sample (21.23 MPa). Similarly, EAB and seal strength of the films incorporated with BLE were ameliorated. X-ray diffraction pattern and FTIR illustrated the shift of amorphous to crystalline behavior and a significant interaction among the BLE and FSG functional groups. Furthermore, the thermal stability of the treated films was not affected significantly however, they showed improved antimicrobial activity with the highest diameter of inhibition zone in the BLE4 sample. This study concluded that the FSG-BLE composite films (BLE4 in particular) can be considered as novel packaging material for food conservation coupled with a potential to enhance the shelf life of perishable food products.

Incorporation of betel leaf extract provides oxidative stability and improves phytochemical, textural, sensory and antimicrobial activities of buffalo meat sausages.

The antioxidant effect of betel leaf extract (BLE) on lipid and protein oxidation, microbial count and physicochemical attributes was investigated in meat sausages during refrigerated storage at 4 ± 1 °C. Buffalo meat sausages were developed after incorporating 0, 250, 500 and 750 mg kg-1 of BLE (BLE0, BLE1, BLE2 and BLE3) respectively. The sausages showed no changes in proximate composition due to BLE inclusion, but there was an improvement in microbial quality, color score, textural properties and lipid and protein oxidative stability. Further, higher sensory scores were observed for the BLE-incorporated samples. The images from scanning electron microscopy (SEM) revealed a reduction in surface roughness and unevenness showing microstructure modification in BLE treated sausages compared to the control sausages. Hence, to improve the storage stability and impede the rate of lipid oxidation in sausages, BLE incorporation proved to be an effective strategy.