RESUMEN
It was produced plant-derived product, an omega-3 acid-enriched substrate (64%). In our study we tested the influence of this preparation, which is supposed a membrane-modifying agent, on the processes of damage to the isolated heart under conditions of ischemia-reperfusion. Animals took this substrate as nutrient addition to usually everyday diet. We assumed disorders in cardiodynamics and contractile functions of the myocardium (we measured a perfusion pressure in coronary vessel, left ventricular pressure and dp/dt) and in structure of cardiomyocytes. All mentioned parameters was much better after ischemia-reperfusion in hearts from animals which took an omega-3 acid-enriched substrate in course of 4 week before experiments than in hearts from control animals. Conclusions. Omega-3 polyunsaturated acids exert protective effect on functioning and structure of the isolated rat heart during ischemia-reperfusion.
Asunto(s)
Ácidos Grasos Omega-3/farmacología , Corazón/efectos de los fármacos , Daño por Reperfusión Miocárdica/prevención & control , Animales , Grasas Insaturadas en la Dieta/aislamiento & purificación , Grasas Insaturadas en la Dieta/farmacología , Grasas Insaturadas en la Dieta/uso terapéutico , Evaluación Preclínica de Medicamentos , Ácidos Grasos Omega-3/aislamiento & purificación , Ácidos Grasos Omega-3/uso terapéutico , Ácidos Grasos Omega-6 , Ácidos Grasos Insaturados/aislamiento & purificación , Ácidos Grasos Insaturados/farmacología , Ácidos Grasos Insaturados/uso terapéutico , Corazón/fisiopatología , Técnicas In Vitro , Peroxidación de Lípido/efectos de los fármacos , Masculino , Contracción Miocárdica/efectos de los fármacos , Daño por Reperfusión Miocárdica/patología , Daño por Reperfusión Miocárdica/fisiopatología , Miocardio/ultraestructura , Aceites de Plantas/aislamiento & purificación , Aceites de Plantas/farmacología , Aceites de Plantas/uso terapéutico , RatasRESUMEN
The effect of phosphocreatine and hydroxamate-linoleate (an inhibitor of lipoxigenase) on development of the pathologic process in coronary vessels with immune (cytotoxic) injury of the heart was studied in the experiments on narcotized dogs. Development of the immune response after administration of cardiac serum resulted in development of large transmural damage of the left ventricle myocardium, increased resistance of coronary vessels and changed coronary vascular reactions, which correlates with changes in arachidonic acid metabolism. Experimental data described in this report demonstrate the efficiency of membrane coronary vessels stabilization and inhibition of a lipoxygenase pathway in arachidonic acid metabolism in protection of immune damage of the heart and coronary vessels.