RESUMEN
Anoectochilus formosanus, a plant native to Taiwan, is used as a folk medicine. It was found that oral administration of A. formosanus extract (AFE) (500 mg/kg) for 4 weeks suppressed bone weight loss and trabecular bone loss in ovariectomized mice, an experimental model of osteoporosis. Although AFE at 12.5 and 25 mug/ml inhibited osteoclast formation in co-culture of osteoblasts and bone marrow cells, AFE did not inhibit the formation of osteoclast progenitor cells and preosteoclast cells in bone marrow cells and RAW264 cells. However, AFE (at 12.5 and 25 microg/ml) decreased RANKL expression. These results suggested that AFE might suppress the bone loss caused by estrogen deficiency through suppression of RANKL expression required for osteoclast formation.
Asunto(s)
Resorción Ósea/tratamiento farmacológico , Resorción Ósea/patología , Orchidaceae , Osteoclastos/citología , Osteoclastos/efectos de los fármacos , Extractos Vegetales/farmacología , Animales , Peso Corporal/efectos de los fármacos , Calcitriol/farmacología , Diferenciación Celular/efectos de los fármacos , Femenino , Fémur/efectos de los fármacos , Fémur/patología , Ratones , Tamaño de los Órganos/efectos de los fármacos , Osteogénesis/efectos de los fármacos , Ovariectomía , Fitoterapia , Extractos Vegetales/uso terapéutico , Ligando RANK/metabolismo , Células Madre/citología , Células Madre/efectos de los fármacosRESUMEN
A novel compound (1) and a known one (2) were isolated from Makomotake, Zizania latifolia infected with Ustilago esculenta, as osteoclast-forming suppressive substances.