The extrageniculate visual pathway generates distinct response properties in the higher visual areas of mice.

BACKGROUND: Visual information conveyed through the extrageniculate visual pathway, which runs from the retina via the superior colliculus (SC) and the lateral posterior nucleus (LPN) of the thalamus to the higher visual cortex, plays a critical role in the visual capabilities of many mammalian species. However, its functional role in the higher visual cortex remains unclear. Here, we observed visual cortical area activity in anesthetized mice to evaluate the role of the extrageniculate pathway on their specialized visual properties. RESULTS: The preferred stimulus velocities of neurons in the higher visual areas (lateromedial [LM], anterolateral [AL], anteromedial [AM], and rostrolateral [RL] areas) were measured using flavoprotein fluorescence imaging and two-photon calcium imaging and were higher than those in the primary visual cortex (V1). Further, the velocity-tuning properties of the higher visual areas were different from each other. The response activities in these areas decreased after V1 ablation; however, the visual properties' differences were preserved. After SC destruction, these preferences for high velocities disappeared, and their tuning profiles became similar to that of the V1, whereas the tuning profile of the V1 remained relatively normal. Neural tracer experiments revealed that each of these higher visual areas connected with specific subregions of the LPN. CONCLUSIONS: The preservation of visual property differences among the higher visual areas following V1 lesions and their loss following SC lesions indicate that pathways from the SC through the thalamus to higher cortical areas are sufficient to support these differences.

The presence of ferric and ferrous iron in the nonheme iron store of resident macrophages in different tissues and organs: histochemical demonstrations by the perfusion-Perls and -Turnbull methods in the rat.

We previously developed the highly sensitive perfusion-Perls and -Turnbull methods to visualize nonheme ferric (Fe (III)) and ferrous (Fe (II)) iron, respectively. The present study used these methods to investigate the possible presence of nonheme iron in the redox (ferric/ferrous) state in the noneheme iron store (phagolysosomes and siderosomes) of resident macrophages in the rat. The perfusion-Perls and -Turnbull methods at pH 0.6 supplemented by DAB intensification intensely stained resident macrophages of different tissues and organs of normal and iron-overloaded rats. The perfusion-Turnbull method, which is specific for nonheme Fe (II), partly stained hemosiderin at pH 5.3, but hardly stained it at the physiological pH, suggesting the presence of some iron in the reduced form, free Fe2+ and/or loosely bound Fe (II), at the intravacuolar pH (5.4+/-0.2) of the phagolysosomes of macrophages. Electron microscopy of the splenic and hepatic macrophages treated by the perfusion-Perls or -Turnbull method showed that Fe (II) deposits were largely distributed along the margin of hemosiderin masses while Fe (III) deposits could also be found within hemosiderin masses.

Perfusion-Perls and -Turnbull methods supplemented by DAB intensification for nonheme iron histochemistry: demonstration of the superior sensitivity of the methods in the liver, spleen, and stomach of the rat.

Perfusion-Perls and -Turnbull methods supplemented by the intensification with 3,3'-diaminobenzidine (+ DAB) enabled stronger and more extensive staining of nonheme iron than the Perls + and Turnbull + DAB methods carried out on tissue sections fixed with 10% formalin in 0.9% saline or PBS. The section- and perfusion-Perls + DAB methods are not specific for the demonstration of nonheme ferric iron but also stain nonheme ferrous iron. However, owing to its high sensitivity, the perfusion-Perls + DAB method would provide useful information about nonheme iron deposition regardless of oxidation states in normal and pathological conditions. The perfusion-Turnbull + DAB method is specifically demonstrable of nonheme ferrous iron and the results from this method showed significant stores of nonheme ferrous iron in the hepatocytes, Kupffer cells, splenic macrophages, and gastric parietal cells of the rat. Since nonheme ferrous iron is considered to be critically involved in free radical generation, the perfusion-Turnbull + DAB method would visualize such populations of cells that are at risk from free radical damage.