Physical activity level of post-stroke individuals that use the Brazilian public health system / Nível de atividade física de indivíduos pós-AVC usuários do sistema público de saúde brasileiro

Abstract Introduction: According to studies from developed countries, post-stroke individuals commonly have a low level of physical activity. Considering the benefits of maintaining a good level of physical activity in these subjects, it is important to provide specific and complete information, based on the assessment of all dimensions of physical activity, which supports interventions. Objective: To compare the physical activity levels between individuals with stroke and matched healthy individuals that use the public health system in Brazil considering the different dimensions of physical activity. Methods: Individuals with stroke (n = 11) and matched healthy individuals (n = 11) were assessed. Physical activity levels, considering all dimensions -duration (> 3 MET), frequency (number of steps) and intensity (mean total energy expenditure per day) - were assessed using SenseWear® monitor for seven days. Descriptive statistics and between-groups comparisons were performed (α = 0.05). Results: The physical activity levels were significantly lower in individuals with stroke when compared to matched healthy individuals, considering all dimensions. The between-group differences in activity duration, frequency, and intensity were 74 minutes/day, 5,274 steps/day, and 2,134kJ/day, respectively. Conclusion: Individuals with stroke users of the Brazilian public health system have lower physical activity levels in different dimensions of physical activity than matched healthy individuals. The assessment of the physical activity level of post-stroke individuals is important for decision making in public health programs.

Resumo Introdução: De acordo com os estudos realizados em países desenvolvidos, indivíduos pós-acidente vascular cerebral (AVC) comumente apresentam baixo nível de atividade física. Considerando os benefícios desses indivíduos manterem um bom nível de atividade física, é importante fornecer informações específicas e completas a partir da avaliação de todas as dimensões da atividade física que guiem as intervenções. Objetivo: Comparar o nível de atividade física de indivíduos pós-AVC e saudáveis pareados, usuários do sistema público de saúde brasileiro, considerando as diferentes dimensões da atividade física. Métodos: Foram avaliados indivíduos pós-AVC (n = 11) e seus pares saudáveis (n = 11). O nível de atividade física, considerando as diferentes dimensões - duração (> 3 equivalentes metabólicos), frequência (número de passos) e intensidade (média do gasto energético total por dia) -, foi avaliado com o monitor SenseWear® por sete dias. Foram utilizadas estatísticas descritivas e comparações entre os grupos (α = 0,05). Resultados: O nível de atividade física foi significativamente menor nos indivíduos pós-AVC comparados aos seus pares saudáveis, considerando todas as dimensões. A diferença entre os grupos na duração, frequência e intensidade da atividade foi de 74 minutos/dia, 5274 passos/dia e 2134 kJ/dia, respectivamente. Conclusão: Indivíduos pós-AVC usuários do sistema público de saúde brasileiro têm menor nível de atividade física nas diferentes dimensões da atividade quando comparados aos seus pares saudáveis. A avaliação do nível de atividade física em indivíduos pós-AVC é importante para a tomada de decisões em programas de saúde pública.

The evaluation of the potential ecotoxicity of pyroligneous acid obtained from fast pyrolysis.

Pyroligneous acid (PA) is a by-product of bio-oil, which is obtained by pyrolysis of the wood. This product has been tested for use in several areas, such as agriculture, as a promising green herbicide; however, there are few scientific data regarding its environmental impacts. For this study, an ecotoxicity testing battery, composed of Daphnia magna acute toxicity test, Allium cepa test and in vitro Comet assay with the rainbow trout gonad-2 cell fish line (RTG-2) were used to evaluate the acute toxicity and genotoxicity of PA obtained from fast pyrolysis of eucalyptus wood fines. The PA presented acute toxicity to D. magna (microcrustacea) with EC50 of 26.12 mg/L, and inhibited the seed germination (EC50 5.556 g/L) and root development (EC50 3.436 g/L) of A. cepa (higher plant). No signs of genotoxicity (chromosomal aberrations and micronuclei in A. cepa and primary DNA lesions in RTG-2 cells) were detected to this product. The acute toxicity and absence of genotoxicity may relate to the molecules found in the PA, being the phenolic fraction the key chemical candidate responsible for the toxicity observed. In addition, daphnids seem to be more sensitivity to the toxicity of PA than higher plants based on their EC50 values. This first ecotoxicological evaluation of PA from fast pyrolysis pointed out the need of determining environmental exposure limits to promote the safer agriculture use of this product, avoiding impacts to living organisms.

The role of calcium in intracellular pathways of rutin in rat pancreatic islets: potential insulin secretagogue effect.

Rutin is a flavonol glycoside with multiple biological activities and it has been demonstrated that rutin modulates glucose homeostasis. In pancreatic ß-cell, an increase in intracellular calcium concentration triggers exocytosis and thus insulin secretion. The aim of the study reported herein was to investigate the effect of rutin associated intracellular pathways on Ca(2+) uptake in isolated rat pancreatic islets. We focused on the acute effects of rutin on in vivo insulin secretion and the in vitro cellular signaling of pancreatic islets related to this effect. The results show that rutin significantly increased glucose-induced insulin secretion in an in vivo treatment. Moreover, it was demonstrated that rutin stimulated Ca(2+) uptake after 10 min of incubation compared with the respective control group. The involvement of L-type voltage-dependent Ca(2+) channels (L-VDCCs) was evidenced using nifedipine, while the use of glibenclamide and diazoxide demonstrated that the ATP-sensitive potassium (KATP) channels are not involved in the rutin action in pancreatic islets. In conclusion, rutin diminish glycemia, potentiate insulin secretion in vivo and significantly stimulates Ca(2+) uptake in rat pancreatic islets. A novel cellular mechanism of action of rutin in Ca(2+) uptake on pancreatic ß-cells was elucidated. Rutin modulates Ca(2+) uptake in pancreatic islets by opening L-VDCCs, alter intracellular Ca(2+), PLC and PKC signaling pathways, characterizing KATP channel-independent pathways. These findings highlight rutin, a dietary adjuvant, as a potential insulin secretagogue contributing to glucose homeostasis.