Effects of cadmium and flooding on the formation of iron plaques, the rhizosphere bacterial community structure, and root exudates in Kandelia obovata seedlings.

In the rhizosphere, plant root exudates (REs) serve as a bridge between plant and soil functional microorganisms, which play a key role in the redox cycle of iron (Fe). This study examined the effects of periodic flooding and cadmium (Cd) on plant REs, the rhizosphere bacterial community structure, and the formation of root Fe plaques in the typical mangrove plant Kandelia obovata, as well as the relationship between REs and Fe redox cycling bacteria. Based on two-way analysis of variance, flooding and Cd had a considerable effect on the REs of K. obovata. DOC, NH4+-N, NO3--N, dissolved inorganic phosphorus, acetic acid, and malonic acid concentrations in REs of K. obovata increased considerably with the increase of Cd concentration under 5 and 10 h flooding conditions. Fe plaque development in the plant root was stimulated by flooding and Cd, although flooding was more effective. After Cd treatment, the ways in which Fe-oxidizing bacteria (FeOB) and Fe-reducing bacteria (FeRB) were enriched in the rhizosphere and rhizoplane of plants were different. Thiobacillus and Sideroxydans (dominant FeOB) were more abundant in the plant rhizosphere, whereas Acinetobacter (dominant FeRB) was more abundant in the rhizoplane. Cd considerably decreased the relative abundance of unclassified_f_Gallionellaceae in the rhizosphere and rhizoplane but dramatically enhanced the relative abundance of Thiobacillus, Shewanella, and unclassified_f_Geobacteraceae. Unclassified_f_Geobacteraceae and Thiobacillus exhibited substantial positive correlations with citric acid and DOC in REs in the rhizosphere and rhizoplane but strong negative correlations with Sideroxydans. The findings indicate that Cd and flooding treatments may play a role in the production and breakdown of Fe plaque in K. obovata roots by affecting the relative abundance of Fe redox cycling bacteria in the rhizosphere and rhizoplane.

Effects of exogenous organic acids and flooding on root exudates, rhizosphere bacterial community structure, and iron plaque formation in Kandelia obovata seedlings.

The rhizosphere of coastal wetland plants is the active interface of iron (Fe) redox transformation. However, coupling mechanism between organic acids (OAs) exuded by plant roots and Fe speciation transformation participated by Fe redox cycling bacteria in the rhizosphere is still unclear. Effects of four common OAs (citric acid, malic acid, tartaric acid, and oxalic acid) on root exudation, rhizosphere bacterial community structure, root Fe plaque, and Fe redox cycling bacterial communities of Kandelia obovata were investigated in this study. Long-term flooding (10 h) was conducive to K. obovata seedlings exuding additional dissolved organic carbon (DOC) and nitrogen and phosphorus organic matter (NH4+-N, NO3--N, and dissolved inorganic phosphorus [DIP]) under each OA level. DOC, NH4+-N, NO3--N, and DIP in root exudates increased significantly with the increase of exogenous OA level. Notably, long flooding time corresponds to an evidently increasing trend. Exogenous OAs also significantly increased contents of formic and oxalic acids in root exudates. Exogenous OAs and flooding enhanced the rhizosphere effect of K. obovata and significantly enhanced bacterial diversity of the rhizosphere and relative abundance of dominant bacteria in rhizoplane. Bacterial diversity in the rhizosphere of K. obovata seedlings was significantly higher than that in the rhizoplane under the same level of OAs and flooding. Fe plaque content of K. obovata root decreased significantly and the relative abundance of typical Fe-oxidizing bacteria, such as Gallionella, unclassified_f__Gallionellaceae, and Sideroxydans, decreased significantly in the rhizosphere but increased significantly in the rhizoplane with the increase of the treatment level of exogenous OAs. This finding is likely due to the Fe3+ reduction caused by acidification of rhizosphere environment after exogenous OA treatment rather than the result of chemotactic colonization of Fe redox cycling bacteria in the rhizoplane.