RESUMEN
OBJECTIVE: To observe the effect of salviandic acid B (SA-B) on MMP-2/9 and TIMP-2 of fibrotic cardiac tissues in rats and explore the action mechanism of SA-B anti-fibrosis of heart. METHOD: Ventricular remodeling model was induced by abdominal aortic banding (AAB) in rats. Rats were randomly divided into 6 groups: normal, model, SA-B high, SA-B middle, SA-B low and captopril control group. Histological changes of heart were observed with hemotoxylin and eosin (H&E) staining and Sirius red staining. Hydroxyproline (Hyp) content in heart tissue was measured by hydrolysis method. Expression of heart tissue collagen NIV, MMP-2/9 and TIMP-2 were analyzed with Western blot The activities of heart tissue MMP-2 were determined with gelatin zymography substrate degradation method. RESULT: SA-B treated groups had lower heart inflammation and lower heart Hyp content; decreased Collagen deposit and alleviated cardiac fibrosis. SA-B treated groups obviously decreased the expression of Collagen IV, MMP-2/9 and TIMP-2. The activity of MMP-2 was decreased in treated SA-B treated groups. CONCLUSION: The mechanism of SA-B action against cardiac fibrosis may be related to down-regulating the expression of TIMP -2 and the activity of MMP-2/9, thus protect the normal basal membrane.
Asunto(s)
Membrana Basal/enzimología , Cardiomegalia/tratamiento farmacológico , Medicamentos Herbarios Chinos/farmacología , Metaloproteinasa 2 de la Matriz/metabolismo , Metaloproteinasa 9 de la Matriz/metabolismo , Inhibidor Tisular de Metaloproteinasa-1/metabolismo , Animales , Membrana Basal/efectos de los fármacos , Cardiomegalia/enzimología , Cardiomegalia/genética , Modelos Animales de Enfermedad , Humanos , Masculino , Metaloproteinasa 2 de la Matriz/genética , Metaloproteinasa 9 de la Matriz/genética , Ratas , Ratas Sprague-Dawley , Inhibidor Tisular de Metaloproteinasa-1/genéticaRESUMEN
OBJECTIVE: To study the protective effect of the total isoflavones from Pueraria lobata (TIP) on secondary osteoporosis induced by dexamethasome (DXM). METHODS: Female SD rats, 4 month aged, were given intramuscularly with 1.5 mg/kg DXM twice once week. The treated groups were orally given TIP 100, 50 or 25 mg.kg-1.d-1 for 6 months. The whole body BMD and BMC were detected at 3 and 6 months. The biodynamics potency, density, ash weight and calcium content of femur were determined at 6 months. RESULTS: (1) At 3 and 6 months, BMDs in TIP 100 mg/kg group were increased by 3.4% and 8.4% than that in model group, BMC were increased by 7.0% and 9.1%, while in TIP 50 mg/kg group by 3.2%, 5.3% and 6.2%, 6.0%. (2) The Fmax and hardness in TIP 100 mg/kg group were enhanced by 10.3% and 10.5%, when in TIP 50 mg/kg group were enhanced by 3.5% and 7.1%. (3) TIP can increase wet and dry weight in slightly extent, but significantly increase wet density and dry density. (4) The ash weight and calcium content in femur were increased by 3.1% and 4.2% in TIP 100 mg/kg group, 4.3% and 6.4% in TIP 50 mg/kg group, while no change in TIP 25 mg/kg group. CONCLUSIONS: TIP can prevent secondary osteoporosis induced by DXM in rats.