The Effects of Selenium-Enriched Probiotics on Growth Performance, Oocysts Shedding, Intestinal Cecal Lesion Scores, Antioxidant Capacity, and mRNA Gene Expression in Chickens Infected with Eimeria tenella.

This study was carried out to investigate the effects of selenium-enriched probiotics (SP) supplementation on growth performance, oocysts shedding, intestinal lesions and antioxidant capacities, and mRNA gene expression of local Chinese yellow male chickens infected with Eimeria tenella. One-day-old 270 chickens were randomly assigned into five groups, each consisting of three replicates with 18 chickens per replicate. Chickens in the negative and positive controls (NC, PC, respectively) received basal diets only (0.11 mg Se/kg), whereas the other groups were supplied basal diets with probiotics and designated as (P, 0.11 mg Se/kg), sodium selenite (SS, 0.41 mg Se/kg), and (SP, 0.41 mg Se/kg) groups. At 21 days of age, except the NC group, all other groups were infected by oral gavage with 1.5 × 104 sporulated E. tenella oocysts per chicken. Three chickens were randomly selected from each group for serum, liver, and cecal specimen collection. The results showed that P, SS, and SP had significant increase weight gain and feed intake. Additionally, these groups showed higher activities of serum superoxide dismutase (SOD) and glutathione peroxidase-1 (GPx1) compared to the PC group, whereas feed conversion ratio (FCR), serum catalase (CAT) activity, and malondialdehyde (MDA) content remained lower. Moreover, P, SS, and SP groups had lower oocyst shedding and cecal lesion scores. Significant upregulation of the glutathione peroxidase-1 (GPx1), glutathione peroxidase-4 (GPx4), Selenium W (SelW), and interferon gamma (IFN-γ) mRNA expression were detected in the SP group, which was then followed by SS when compared to the P group, whereas mRNA expression down-regulated in the PC group compared to NC, P, SS, and SP. In the NC and P groups, there were no significant differences in mRNA expression, except that IFN-γ mRNA level upregulated in P. We concluded that selenium-enriched probiotic supplementation has profound effects in enhancing the growth performance, antioxidant capacities, mRNA gene expression, reduced of oocysts shedding, and the cecal lesion scores of chickens and do provide protection against E. tenella.

Effects of Selenium-Enriched Probiotics on Lipid Metabolism, Antioxidative Status, Histopathological Lesions, and Related Gene Expression in Mice Fed a High-Fat Diet.

A total of 80 female albino mice were randomly allotted into five groups (n = 16) as follows: (A) normal control, (B) high-fat diet (HFD),; (C) HFD + probiotics (P), (D) HFD + sodium selenite (SS), and (E) HFD + selenium-enriched probiotics (SP). The selenium content of diets in groups A, B, C, D, and E was 0.05, 0.05, 0.05, 0.3, and 0.3 µg/g, respectively. The amount of probiotics contained in groups C and E was similar (Lactobacillus acidophilus 0.25 × 10(11)/mL and Saccharomyces cerevisiae 0.25 × 10(9)/mL colony-forming units (CFU)). The high-fat diet was composed of 15 % lard, 1 % cholesterol, 0.3 % cholic acid, and 83.7 % basal diet. At the end of the 4-week experiment, blood and liver samples were collected for the measurements of lipid metabolism, antioxidative status, histopathological lesions, and related gene expressions. The result shows that HFD significantly increased the body weights and liver damages compared to control, while P, SS, or SP supplementation attenuated the body weights and liver damages in mice. P, SS, or SP supplementation also significantly reversed the changes of alanine aminotransferase (AST), aspartate aminotransferase (ALT), total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL), total protein (TP), high-density lipoprotein (HDL), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalasa (CAT), and malondialdehyde (MDA) levels induced by HFD. Generally, adding P, SS, or SP up-regulated mRNA expression of carnitine palmitoyltransferase-I (CPT1), carnitine palmitoyltransferase II (CPT2), acetyl-CoA acetyltransferase II (ACAT2), acyl-coenzyme A oxidase (ACOX2), and peroxisome proliferator-activated receptor alpha (PPARα) and down-regulated mRNA expression of fatty acid synthase (FAS), lipoprotein lipase (LPL), peroxisome proliferator-activated receptor gamma (PPARγ), and sterol regulatory element-binding protein-1 (SREBP1) involved in lipid metabolism. Among the group, adding SP has a maximum effect in improving lipid metabolism, antioxidative status, histopathological lesions, and related gene expression in mice fed a HFD.