RESUMEN
The exposure of human lymphoid leukemia Molt 4B cells to sesaminol, a component of sesame oil led to both growth inhibition and the induction of apoptosis. Morphological change showing apoptotic bodies was observed in the cells treated with sesaminol. The fragmentation of DNA by sesaminol to oligonucleosomal-sized fragments that are characteristics of apoptosis was observed to be concentration- and time-dependent. These findings suggest that growth inhibition of Molt 4B cells by sesaminol results from the induction of apoptosis in the cells.
Asunto(s)
Apoptosis/efectos de los fármacos , Dioxoles/farmacología , Furanos/farmacología , Leucemia Linfoide/patología , División Celular/efectos de los fármacos , Fragmentación del ADN/efectos de los fármacos , ADN de Neoplasias/efectos de los fármacos , ADN de Neoplasias/genética , ADN de Neoplasias/metabolismo , Relación Dosis-Respuesta a Droga , Humanos , Leucemia Linfoide/tratamiento farmacológico , Aceite de Sésamo/química , Células Tumorales CultivadasRESUMEN
The exposure of human lymphoid leukemia Molt 4B cells to sesamolin, a component of sesame seed led to both growth inhibition and the induction of apoptosis. Morphological change showing apoptotic bodies was observed in the cells treated with sesamolin. The fragmentation of DNA by sesamolin to oligonucleosomal-sized fragments that are characteristics of apoptosis was observed to be concentration- and time-dependent. These findings suggest that growth inhibition of Molt 4B cells by sesamolin results from the induction of apoptosis in the cells.
Asunto(s)
Apoptosis/efectos de los fármacos , Dioxoles/farmacología , Inhibidores de Crecimiento/farmacología , Leucemia Linfoide/patología , Semillas/química , Aceite de Sésamo/química , Antineoplásicos/farmacología , División Celular/efectos de los fármacos , Humanos , Aceite de Sésamo/farmacología , Células Tumorales CultivadasRESUMEN
The exposure of human lymphoid leukemia Molt 4B cells to sesamin and episesamin which were isolated from unroasted sesame seed oil and identified by MS, and 1H and 13C-NMR, led to both growth inhibition and the induction of programmed cell death (apoptosis). Morphological change showing apoptotic bodies was observed in the Molt 4B cells treated with sesamin and episesamin. The fragmentations by sesamin and episesamin of DNA to oligonucleosomal-sized fragments that are characteristics of apoptosis were observed to be concentration-dependent, respectively. Moreover, the amount of the DNA fragments in the sesamin-treated cells was increased from 2 days, while that in the episesamin-treated cells was elevated at 3 days after addition of the compounds. These findings suggest that growth inhibitions by sesamin and episesamin of Molt 4B cells result from the induction of apoptosis in the cells.
Asunto(s)
Antineoplásicos/farmacología , Apoptosis , Dioxoles/farmacología , Lignanos/farmacología , Aceite de Sésamo/farmacología , Antineoplásicos/química , Antineoplásicos/aislamiento & purificación , Fragmentación del ADN , Dioxoles/química , Dioxoles/aislamiento & purificación , Humanos , Leucemia Linfoide , Lignanos/química , Lignanos/aislamiento & purificación , Espectroscopía de Resonancia Magnética , Espectrometría de Masas , Microscopía Fluorescente , Aceite de Sésamo/química , Aceite de Sésamo/aislamiento & purificación , Células Tumorales CultivadasRESUMEN
To investigate the possible participation of immunoglobulin E (IgE) in the autoimmune process of Graves' disease, incidence of elevation of serum IgE level, TSH receptor antibody (TRAb), and thyroid status were studied in 66 patients with hyperthyroid Graves' disease, 54 patients with Hashimoto's thyroiditis, 19 patients with bronchial asthma, and 15 patients with pollen allergy. In hyperthyroid Graves' patients, elevation of serum IgE levels (> or = 170 U/mL) was found in 19 of 66 patients (29%), 11 of whom had hereditary and/or allergic conditions. Elevations of serum IgE levels were found in 63% of patients with bronchial asthma and in 40% of patients with pollen allergy. Mean values of serum IgE were the same in patients with hyperthyroid Graves' disease and with bronchial asthma. During methimazole treatment TRAb decreased without fluctuation of IgE levels in both groups. The decrease in TRAb was significantly greater in patients with normal IgE than in patients with IgE elevation. After prednisone administration, reduction in TRAb was greater in patients with normal IgE than that in patients with IgE elevation. High incidence of IgE elevation in hyperthyroid Graves' disease and slower reduction in TRAb in association with IgE elevation suggest a difference in the autoimmune processes in Graves' disease with and without elevation of IgE.