RESUMEN
Two ester-type prostaglandin oligomeric compounds were synthesized, one from prostaglandin E1 (termed MR-356) and the other from prostaglandin B2 (termed OC-5186). Using in vivo [31P]MRS, the protective effects of these oligomers on forebrain ischemia (15 min) were evaluated in a rat model. Forebrain ischemia caused a decrease in intracellular high energy phosphates and intracellular pH (pHi) in the control and compounds-treated groups, but changes of these values in the OC-5186-treated group were significantly smaller than those in the control group. Moreover, the cerebral energy metabolism of the OC-5186-treated group returned to the preischemia level more rapidly than in the control group after forebrain ischemia. MR-356 had some effects, but the differences were not significant.
Asunto(s)
Alprostadil/análogos & derivados , Encéfalo/metabolismo , Ataque Isquémico Transitorio/prevención & control , Fosfatos/metabolismo , Fosfocreatina/metabolismo , Prostaglandinas B/farmacología , Prostaglandinas/farmacología , Adenosina Trifosfato/metabolismo , Análisis de Varianza , Animales , Encéfalo/efectos de los fármacos , Metabolismo Energético/efectos de los fármacos , Concentración de Iones de Hidrógeno , Ataque Isquémico Transitorio/metabolismo , Espectroscopía de Resonancia Magnética/métodos , Masculino , Fósforo , Ratas , Ratas Endogámicas , Factores de TiempoRESUMEN
In vivo 31P-NMR spectroscopy (31P-MRS) was used to study the metabolism of phosphate compounds in rat liver under various conditions. The changes in hepatic concentrations of ATP and inorganic phosphate (Pi) or intracellular pH (pHi) were monitored during hypovolemic shock with or without the infusion of catecholamines. Rapid decreases in the ATP level and pHi with a concomitant increase of Pi were observed upon induction of the hypovolemic shock. Dopamine infusion markedly improved the liver ATP concentration and intracellular acidosis, but epinephrine or norepinephrine were without effects. The present results suggest that dopamine increases abdominal blood flow and improves the energy metabolism in the liver during hypovolemic shock.