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Artículo en Inglés | MEDLINE | ID: mdl-21243929

RESUMEN

BACKGROUND: Schefflera leucantha Viguier is used as a traditional medicine in Thailand and China to relieve chronic cough and asthma. However, little is known about its anti-allergic effects. OBJECTIVE: This study was designed to investigate the effects of S leucantha ethanol extract (SLEE) on chemokine production by epidermal Langerhans cells (LCs) stimulated with peptidoglycan (PEG) from Staphylococcus aureus and histamine release from mast cells. METHODS: LCs were purified from murine epidermal cells using the panning method with anti-IA(d) monoclonal antibody. Chemokine production by LCs was investigated by reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay (ELISA). Mast cells for histamine release assay were induced by long-term culture of mouse spleen cells. Histamine release from these mast cells was measured by a competitive ELISA. RESULTS: Production of the eosinophil chemoattractant CCL5 and the type 2 T helper (TH2)-associated chemokine CCL17 from PEG-stimulated LCs was significantly inhibited by SLEE. Furthermore, SLEE significantly decreased the release of histamine from mast cells by IgE-mediated degranulation. CONCLUSION: These results suggest that S leucantha may offer a new therapeutic approach for the control of atopic dermatitis associated with S aureus colonization through inhibition of the production of allergic mediators.


Asunto(s)
Antialérgicos/farmacología , Araliaceae , Quimiocina CCL17/biosíntesis , Quimiocina CCL5/biosíntesis , Células de Langerhans/efectos de los fármacos , Mastocitos/efectos de los fármacos , Extractos Vegetales/farmacología , Animales , Dermatitis Atópica/tratamiento farmacológico , Femenino , Liberación de Histamina/efectos de los fármacos , Células de Langerhans/fisiología , Mastocitos/fisiología , Ratones , Ratones Endogámicos BALB C , Peptidoglicano/farmacología
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