RESUMEN
We report a case in which medicinal leeches (Hirudo medicinalis) were used to rescue a traumatic nasal flap from venous congestion and loss. A healthy 18-year-old had a bicycling accident and, in addition to other facial injuries, presented with a complex full thickness nasal laceration. After he had been resuscitated and the flap sutured, he was treated with two leeches four times a day for 48 h. His recovery was satisfactory and uncomplicated. Oral and maxillofacial surgeons should be familiar with the use of leeches, as they have a small but occasionally important role in the management of both traumatic and reconstructive flaps.
Asunto(s)
Sanguijuelas , Nariz/lesiones , Adolescente , Animales , Ciclismo/lesiones , Traumatismos Faciales/cirugía , Humanos , Masculino , Nariz/irrigación sanguínea , Nariz/cirugía , Procedimientos de Cirugía Plástica , Reimplantación , Supervivencia TisularRESUMEN
The effects of Panax ginseng extract, ginseng saponins, and some other triterpenoid saponins on glucose uptake were examined by using sheep erythrocytes. Initial rates of glucose transport were determined by measurements of 2-deoxy-D-glucose (2-DG) uptake. From kinetic analysis apparent Km and Vmax values of facilitated glucose transport in sheep erythrocytes were calculated as 2.3 +/- 0.08 mM and 1.4 +/- 0.05 nmol/min/10(9) cells. The results showed that ginseng extract stimulated glucose uptake in sheep erythrocytes dose-dependently. Ginseng saponins, in general, also stimulated glucose transport. The maximum effect was observed at 1 microM of ginsenoside Rb1 showing an increase of 24 +/- 5% above basal activity. However, ginsenoside Rg3, chikusetsusaponin Ia, and glycyrrhetic acid induced significant inhibitory effects on glucose transport in sheep erythrocytes.
Asunto(s)
Eritrocitos/efectos de los fármacos , Proteínas de Transporte de Monosacáridos/efectos de los fármacos , Panax , Plantas Medicinales , Saponinas/farmacología , Triterpenos/farmacología , Animales , Eritrocitos/metabolismo , Ginsenósidos , Glucosa/metabolismo , Interacciones de Hierba-Droga , Técnicas In Vitro , Proteínas de Transporte de Monosacáridos/metabolismo , Ovinos , Relación Estructura-ActividadRESUMEN
The effect of plant extracts on D-glucose uptake by Ehrlich ascites tumour cells was examined. Among the 23 extracts of medicinal plants, five samples inhibited, and six samples activated, the uptake significantly. From one of the active plants, Lagerstroemia speciosa, two triterpenoids, colosolic acid and maslinic acid were isolated. Colosolic acid was shown to be a glucose transport activator. Since this compound was known to have hypoglycemic activity, our simple in vitro bioassay method can at least be used as a first screening for anti-diabetic activity.