Methodologic considerations in the measurement of glycemic index: glycemic response to rye bread, oatmeal porridge, and mashed potato.

BACKGROUND: Methodologic choices affect measures of the glycemic index (GI). The effects on GI values of blood sampling site, reference food type, and the number of repeat tests have been insufficiently determined. OBJECTIVE: The objective was to study the effect of methodologic choices on GI values. Comparisons were made between venous and capillary blood sampling and between glucose and white bread as the reference food. The number of tests needed for the reference food was assessed. Rye bread, oatmeal porridge, and instant mashed potato were used as the test foods. DESIGN: Twelve healthy volunteers were served each test food once and both reference foods 3 times at 1-wk intervals in a random order after they had fasted overnight. Capillary and venous blood samples were drawn at intervals for 3 h after each study meal. RESULTS: GIs and their CVs based on capillary samples were lower than those based on venous samples. Two tests of glucose solution as the reference provided stable capillary GIs for the test foods. The capillary GIs did not differ significantly when white bread was used as the reference 1, 2, or 3 times, but the variation was lower when tests were performed 2 and 3 times. Capillary GIs with white bread as the reference were 1.3 times as high as those with glucose as the reference. The capillary GIs of rye bread, oatmeal porridge, and mashed potato were 77, 74, and 80, respectively, with glucose as the reference. CONCLUSIONS: Capillary blood sampling should be used in the measurement of GI, and reference tests with glucose or white bread should be performed at least twice.

Black currant seed oil and fish oil supplements differ in their effects on fatty acid profiles of plasma lipids, and concentrations of serum total and lipoprotein lipids, plasma glucose and insulin.

European diets provide a suboptimal intake of eicosapentaenoic (20:5n3) and docosahexaenoic (22:6n3) acids, which are derived mainly from fish oils. The present study indicates that black currant seed oil, which contains 14.5% alpha-linolenic (18:3n3), 12.6% gamma-linolenic (18:3n6), 47.5% linoleic (18:2n6) and 2.7% stearidonic (18:4n3) acids, could potentially serve as alternative to fish oil as a n3 fatty acid source. Fifteen healthy females participated in a randomized, double-blind, crossover study including two 4-week periods with either 3 g/day of black currant seed oil or 2.8 g/day of fish oil separated by a 4-week washout period. The results show that black currant seed oil supplementation increased the proportion of 18:3n6 in triacylglycerols (TAG) and cholesteryl esters (CE), and that of dihomo-gamma-linolenic (20:3n6) in TAGs, CEs and glycerophospholipids (GPL) (P<.05). Proportion of 18:3n6 was higher (P<.05) after black currant seed oil than after fish oil in TAGs and CEs, and that of 20:3n6 in TAGs, CEs and GPLs. Black currant seed oil supplementation caused only minor changes in the proportions of 20:5n3 or 22:6n3. Serum levels of LDL cholesterol were lower (P<.05) after black currant seed oil compared to fish oil. Plasma glucose concentration decreased during the fish oil supplementation (P<.05).

Triacylglycerol molecular weight and to a lesser extent, fatty acid positional distribution, affect chylomicron triacylglycerol composition in women.

Postprandial composition of chylomicron triacylglycerols (TAG) and their clearance may be affected by the molecular weight of TAG, their fatty acid (FA) combinations and the positional distribution of FA in TAG. Delayed postprandial TAG clearance is a risk factor for cardiovascular disease. However, due to the complexity of traditional analysis methods, the composition of individual TAG molecules is frequently overlooked. In this study, chylomicron TAG molecular weight distribution and regioisomerism were followed in 10 healthy female volunteers after two fat loads with identical FA composition but different positional distributions (palm oil and transesterified palm oil). An efficient tandem mass spectrometric method of analysis was applied. During the 6-h observation period, the relative concentrations of TAG with 48:2 [48 acyl carbons and 2 double bonds (ACN:DB)], 50:3 and 50:2 decreased, whereas the proportions of 48:0 (tripalmitin), 52:3 and 54:4 remained constant and the proportion of 54:3 (triolein) increased (P < 0.05). The existence of seven regioisomers containing palmitic, oleic and linoleic acids in different sn-positions was studied. The amount of 1,3-dipalmitoyl-2-oleoyl-sn-glycerol was less (P < 0.05) 1.5 h postprandially than at 2-5 h after palm oil, and less (P < 0.05) at 1.5 h than at 2-6 h after transesterified palm oil. This may be an indication of a loss of palmitic acid in the gut. Taken together, TAG molecular weight composition and to a lesser extent, positional distribution, seem to affect the rates of chylomicron TAG clearance in humans.