RESUMEN
Oxidative damage to sperm during cooled storage is a significant issue, and selenium with antioxidant potential could be a solution. Moreover, nano-sized selenium offers more advantages compared to its ionic forms. This research aimed to assess the impact of selenium nanoparticles (SeNPs) supplemented in the INRA96 extender on the quality of Turkmen stallion sperm and lipid peroxidation during 72 h of cooled storage. A total of 25 ejaculates were treated using different concentrations of SeNPs, including no SeNPs (Control), 0.5 µM SeNPs (SeNPs 0.5), 1.0 µM SeNPs (SeNPs 1.0), and 1.5 µM SeNPs (SeNPs 1.5). The samples were then evaluated for sperm quality characteristics and lipid peroxidation. The results indicated a significant decrease (P < 0.05) in total and progressive motility, viability, and plasma membrane functionality after 48 h of cooled storage, along with an increase (P < 0.05) in spermatozoa abnormality and malondialdehyde (MDA) levels as the cooled storage time increased. However, SeNPs demonstrated an improvement (P < 0.05) in sperm total motility after 24 h of cooled storage, progressive motility throughout the entire 72-hour period, functionality of the plasma membrane after 48 hours of cooled storage, spermatozoa abnormality after 48 h of cooled storage, and semen MDA levels throughout the cooled storage (P < 0.05). In conclusion, the enrichment of the INRA96 extender with nano-sized selenium can enhance the quality of Turkmen stallion sperm during storage at 5 °C by increasing total, progressive, and curvilinear motilities, improving plasma membrane functionality, and reducing sperm abnormalities and lipid peroxidation.