Immunoinformatics design of B and T-cell epitope-based SARS-CoV-2 peptide vaccination.

SARS-COV-2 is a virulent respiratory virus, first identified in China (Wuhan) at the end of 2019. Scientists and researchers are trying to find any possible solution to this deadly viral disease. Different drug source agents have been identified, including western medicine, natural products, and traditional Chinese medicine. They have the potential to counteract COVID-19. This virus immediately affects the liver and causes a decrease in oxygen levels. In this study, multiple vacciome approaches were employed for designing a multi-epitope subunit vaccine for battling against SARS-COV-2. Vaccine designing, immunogenicity, allergenic, and physico-chemical assessment were performed by using the vacciome approach. The vaccine design is likely to be antigenic and produce potent interactions with ACE2 and NSP3 receptors. The developed vaccine has also been given to in-silico cloning models and immune response predictions. A total number of 12 CTL and 12 HTL antigenic epitopes were predicted from three selected covid-19 virulent proteins (spike protein, nucleocapsid protein, and membrane proteins, respectively) based on C-terminal cleavage and MHC binding scores. These predicted epitopes were amalgamated by AYY and GPGPG linkers, and a ß-defensins adjuvant was inserted into the N-terminus of this vaccine. This analysis shows that the recommended vaccine can produce immune responses against SARS-COV-2. Designing and developing of the mentioned vaccine will require further experimental validation.

Ethno veterinary uses of medicinal plants of district Karak, Pakistan.

ETHNOPHARMACOLOGICAL RELEVANCE: In the study area, the traditional knowledge regarding the uses of local wild medicinal plants for treating diseases of domestic animals and birds is totally in the custody of elders of the existing community. The young ones are not much aware about such important practices. AIM OF THE STUDY: The main aim of the study was to document and to release this knowledge from the custody of elders and share with the community. MATERIALS AND METHODS: Total 115 people between 20 and 80 years of age were interviewed and information was collected through semi-structured questionnaires. The data obtained were quantitatively analyzed using the use value (UV) formula. The collected specimens were pressed, dried, preserved, mounted on Herbarium sheets, identified properly and were submitted in the Herbarium, Department of Botany, Hazara University, Mansehra, Pakistan. RESULTS: With the co-ordination and cooperation of the local people, 46 plant species of 42 genera belonging to 31 families were collected, 3 were monocotyledons while 43 plant species belonged to dicotyledonae class. Considering taxonomic characteristics, it was confirmed that 12 trees, 10 shrubs and 22 herbs were commonly used by the local people in ethno veterinary practices. Two plants like Cistanche tubulosa and Cuscuta reflexa from family Orobanchaceae and family Cuscutaceae respectively lack chlorophyll and are parasites on host plants like Doedonia, Ziziphus, Calligonum and Calotropis. The powder of both plants showed great ethno veterinary value. The parts of 46 plant species commonly used for ethno veterinary practices were whole plants (32.60%), leaves (26.08%), fruits (17.39%), stems (13.04%) and roots (10.86%). Medicinal plants were administered through various routes i.e. oral (78.26%), skin (17.21%) and smoke (4.34%). CONCLUSION: The traditional knowledge of local plants of ethno veterinary values is mainly possessed by elders and transmitted from generation to generation with chances of elimination of such traditional knowledge due to less awareness. The present study was designed to document this ethno veterinary related knowledge and to share it with community members for use in future.