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Int J Oncol ; 23(2): 303-10, 2003 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-12851678

RESUMEN

The serum level of beta1,4-galactosyltransferase (beta1,4-GalT) is increased in both malignancy and benign diseases. Galactosyltransferase associated with tumor (GAT) is one of the soluble forms of beta1,4-GalT, and is a marker of ovarian cancer with a high specificity. GAT and normal soluble beta1,4-GalT are both derived from the same membrane-bound form of the enzyme. This study investigated the mechanism of GAT elevation in patients with ovarian cancer. The serum levels of GAT and normal beta1,4-GalT were measured using specific monoclonal antibodies. In addition, nude mice bearing human ovarian cancer were used to assess the kinetics of tumor-derived enzymes. GAT and normal beta1,4-GalT were both detected in ovarian cancer patients, but only GAT reflected the tumor status. In tumor-bearing nude mice, both soluble forms of beta1,4-GalT were released from tumor cells, but the half-life of GAT was far shorter than that of normal beta1,4-GalT. Addition of serum from healthy women to colostrum (which has a high GAT content) reduced the GAT level, while adding patient serum caused a significantly smaller reduction of GAT. Addition of the serum from mouse which includes no human beta1,4-GalT to colostrum also reduced the GAT level with no significant change of total soluble beta1,4-GalT. These findings indicate that human serum contains certain factors that decrease the GAT level, but these factors are inhibited in ovarian cancer patients so that a high GAT level persists. It seems that the decrease of GAT occurs as a result of conversion into normal beta1,4-GalT.


Asunto(s)
Biomarcadores de Tumor/sangre , N-Acetil-Lactosamina Sintasa/sangre , Neoplasias Ováricas/enzimología , Animales , Biomarcadores de Tumor/genética , Northern Blotting , Calostro/enzimología , Femenino , Galactosiltransferasas/sangre , Galactosiltransferasas/genética , Humanos , Técnicas para Inmunoenzimas , Ratones , Ratones Endogámicos BALB C , Ratones Desnudos , Persona de Mediana Edad , N-Acetil-Lactosamina Sintasa/genética , Neoplasias Experimentales/enzimología , ARN Mensajero/metabolismo , Células Tumorales Cultivadas
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