RESUMEN
OBJECTIVE: Erythrosine (E127), a synthetic food dye containing iodine and sodium, has often been used inside packaged foods and beverages in Turkey and many other countries. We evaluated the effects of erythrosine on neural tube development in early-stage chicken embryos. METHODS: The study included 4 groups, with a total of 80 embryos: a control group, a normal saline group, a half-dose group, and a high-dose group. After 30 hours of incubation, saline and erythrosine solution was injected under the embryonic discs. At the end of 72 hours, the embryos were excised and evaluated macroscopically and histopathologically. RESULTS: Neural tube defects were detected in the erythrosine-administered groups with statistically significant differences. In contrast, the embryos in the control and saline groups displayed normal development. CONCLUSIONS: Erythrosine increased the risk of neural tube defects in early-stage chicken embryos, even at half of the approved dose.
Asunto(s)
Eritrosina/farmacología , Colorantes Fluorescentes/farmacología , Defectos del Tubo Neural/embriología , Tubo Neural/efectos de los fármacos , Anomalías Inducidas por Medicamentos/embriología , Anomalías Inducidas por Medicamentos/etiología , Animales , Embrión de Pollo , Desarrollo Embrionario/efectos de los fármacos , Tubo Neural/embriología , Defectos del Tubo Neural/inducido químicamenteRESUMEN
AIM: The dose dependent effects of alpha lipoic acid (α-LA) were investigated morphologically on rat vasospasm model. MATERIAL AND METHODS: 32 rats were divided into four groups: group I=control; group II=vasospasm; group III=vasospasm +low dose (20 mg/kg) intraperitoneal α-LA administered; and group IV=vasospasm +high dose (100 mg/kg) intraperitoneal α-LA administered. Histological and morphometric examinations were carried out for each groups under light microscope. RESULTS: The mean vascular wall thickness displayed significant increase in group II and III compared with group I (p < .05). Statistical comparison of group II and IV, regarding vascular wall thickness showed a significant decrease in group IV, and regarding vascular lumen area showed a significant increase in group IV (p < .05). CONCLUSION: It is demonstrated α-LA reduces the effects of vasospasm in high dose treatment group by decreasing the wall thickness and increasing the lumen surface area. The present study suggests that adequate dose of α-LA is a potential therapeutic agent in experimental vasospasm model.