RESUMEN
As a pharmaceutical plant with multi-bioactivity, Ageratum conyzoides appears to be a valuable agricultural resource. In this study, the complete mitochondrial (mt) genome of A. conyzoides was sequenced through Illumina sequencing method, and the mt genome was recovered after de novo assembly and annotation. The results showed that the 219,198 bp mt genome has a total of 52 genes, including 30 protein-coding genes, 3 rRNA genes and 19 tRNA genes. The overall GC content of this mitogenome is 45.4%. By phylogenetic analysis using maximum-likelihood (ML) method, A. conyzoides showed the closest relationship with Diplostephium hartwegii in the family of Asteroideae.
RESUMEN
Four natural compounds were obtained by concentrating, separating and purifying from the Folium isatidis. These natural compounds have been characterized by elemental analysis, IR spectrum, NMR and single-crystal X-ray diffraction analysis. The results show that these natural compounds are 4(3H)-quinazolinone (I), 2,4(1H,3H)-quinazolinedione (II), methyl 3,4-dihydro-4-oxoquinazoline-2-carboxylate (III) and ethyl 3,4-dihydro-4-oxoquinazoline-2-carboxylate (IV). The antibacterial activity experiment showed that I and II had better activity against Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Salmonella than III, IV and other multiple components, because III and IV have long branches and steric hindrance effect. Compounds I and II have planar structure, which can more easily combine with these bacteria and kill them. The above results have good guiding significance for studying the antibacterial activity for single components or mixtures from natural origin.
Asunto(s)
Antibacterianos/farmacología , Productos Biológicos/farmacología , Medicamentos Herbarios Chinos/farmacología , Isatis/química , Antibacterianos/química , Antibacterianos/aislamiento & purificación , Bacillus subtilis/efectos de los fármacos , Productos Biológicos/química , Productos Biológicos/aislamiento & purificación , Cristalografía por Rayos X , Medicamentos Herbarios Chinos/química , Medicamentos Herbarios Chinos/aislamiento & purificación , Escherichia coli/efectos de los fármacos , Pruebas de Sensibilidad Microbiana , Modelos Moleculares , Hojas de la Planta/química , Salmonella/efectos de los fármacos , Staphylococcus aureus/efectos de los fármacosRESUMEN
OBJECTIVE: To study the chemical constituents of Cardiospermum halicacabum. METHODS: The constituents were isolated and purified by silica gel and polyamide, their chemical structures were identified by physicochemcial properties and spectral methods. RESULTS: Eight compounds were separated and identified as: pentadecanoie acid (1), apigenin (2), protocatechuic acid (3), protocatechualdehyde (4), hentriacontanol (5), calycosin (6), rutin (7), quercetin (8). CONCLUSION: All compounds are isolated from this plant for the first time.