[A case of subacute combined degeneration of spinal cord caused by inhaling laughing gas].

Laughing gas (Nitrogen monoxide) is currently abused due to its low price and easy availability. This article discussed the clinical manifestations of a patient with subacute combined degeneration of the spinal cord caused by inhalation of laughing gas. The patient developed numbness of extremities, unstable walking, and decreased serum vitamin B(12) level. MRI of the cervical spine showed abnormal signals in the lateral and posterior cords of the cervical spinal cord (C2-6) , neuroelectrophysiological examination showed peripheral nerve damage in the extremities. After treatment with vitamin B(12) supplementation, the patient's condition gradually improved. Clinicians diagnose subacute combined degeneration of the spinal cord, especially when the patient has no gastrointestinal disease, diet, malnutrition, etc., they need to carefully inquire about the history of nitrous oxide inhalation to avoid missed diagnosis.

Methionine and selenium yeast supplementation of the maternal diets affects antioxidant activity of breeding eggs.

Four hundred fifty 52-wk-old Langshan layer hens (dual-purpose type, an indigenous poultry breed of China) were randomly divided into 9 treatments with 5 replicates in each treatment. Birds were fed corn-soybean diets (0.13 mg of Se/kg) supplemented with 0, 0.30, and 0.60 mg/kg of Se from Se yeast and 3.2, 4.0, and 5.4 g of dl-Met/kg, respectively. Increasing Se yeast supplementation significantly increased Se concentration in the egg yolk (P < 0.01) and the Se concentration of the 3.2 g of Met/kg treatment was higher than those of the 4.0 and 5.4 g of Met/kg treatments. Adding 0.3 mg of Se/kg to the diet significantly increased glutathione peroxidase (GSH-Px) activity in the egg yolk compared with 0 and 0.6 mg of Se/kg (P < 0.01) and increasing Se yeast supplementation significantly increased the GSH-Px activity in the egg albumen (P < 0.01). Increasing Met supplementation significantly decreased the GSH-Px activity in both the yolk and the albumen of the eggs (P < 0.01). Methionine supplemented at 3.2 and 4.0 g/kg significantly increased glutathione concentration in the egg yolk compared with 5.4 g of Met/kg (P < 0.01) and increasing Met supplementation increased the glutathione concentration in the egg albumen. Increasing Met supplementation significantly decreased malondialdehyde concentration in the egg yolk (P < 0.01) and Se supplemented at 0 and 0.6 mg/kg increased the malondialdehyde concentrations in the egg yolk compared with 0.3 mg of Se/kg (P < 0.01). Methionine supplemented at 4.0 and 5.4 g/kg significantly decreased carbonyl concentration compared with 3.2 g of Met/kg. The conclusion was drawn that Se yeast and Met supplementation of the maternal diets could enhance antioxidant activity of breeding eggs.

Comparative study of SPORL and dilute-acid pretreatments of spruce for cellulosic ethanol production.

The performance of two pretreatment methods, sulfite pretreatment to overcome recalcitrance of lignocellulose (SPORL) and dilute acid (DA), was compared in pretreating softwood (spruce) for fuel ethanol production at 180 degrees Celsius for 30 min with a sulfuric acid loading of 5% on oven-dry wood and a 5:1 liquor-to-wood ratio. SPORL was supplemented with 9% sodium sulfite (w/w of wood). The recoveries of total saccharides (hexoses and pentoses) were 87.9% (SPORL) and 56.7% (DA), while those of cellulose were 92.5% (SPORL) and 77.7% (DA). The total of known inhibitors (furfural, 5-hydroxymethylfurfural, and formic, acetic and levulinic acids) formed in SPORL were only 35% of those formed in DA pretreatment. SPORL pretreatment dissolved approximately 32% of the lignin as lignosulfonate, which is a potential high-value co-product. With an enzyme loading of 15 FPU (filter paper units) per gram of cellulose, the cellulose-to-glucose conversion yields were 91% at 24h for the SPORL substrate and 55% at 48 h for the DA substrate, respectively.

Effects of selenium and methionine supplementation of breeder hen diets on selenium concentration and oxidative stability of lipids in the thigh muscles of progeny.

The effects of dietary supplementation to female chickens with selenium (Se) and methionine (Met) on the next generation were studied. Lang-shan breeding hens (450) were obtained at 52 wk of age and randomly allotted to 9 treatments; 5 replicates of each treatment were carried out. The breeders were fed a basal corn-soybean meal diet (0.13 mg Se/kg) supplemented with 0, 0.30, or 0.60 mg/kg Se from Sel-Plex and 0.32%, 0.40%, or 0.54% Met for the 30-d adapting period and 70-d experiment period. Se and glutathione (GSH) concentrations, glutathione peroxidase (GSH-Px) activity, and the oxidative stability of muscular lipids of 90-d progeny were determined by testing the TBARS values. When breeders received the highest levels of Met or Se, GSH-Px activity was decreased, the Se concentration and the oxidative stability of muscular lipids were increased with the supplementation of Se or Met. When breeder hens were given a Met-deficient diet, supplementing with Se decreased the Se deposition in progeny thigh. With regard to lipid oxidation, 0.3 mg/kg maternal dietary Se supplementation decreased the oxidative stability of muscle lipid and 0.6 mg/kg Se supplementation showed no difference from the control. When breeders were fed a Se-deficient diet, the GSH-Px activity was increased significantly and the oxidative stability of progeny muscles was decreased with the supplementation of Met. It was concluded that supplementation of the maternal diet with higher Se and Met can increase Se deposition in progeny muscle and lead to more effective protection against lipid oxidation in progeny thighs.

Methionine and selenium yeast supplementation of the maternal diets affects color, water-holding capacity, and oxidative stability of their male offspring meat at the early stage.

Four hundred fifty 52-wk-old Lang-shan breeding hens (dual-purpose type, an indigenous poultry breed of China) were randomly divided into 9 treatments with 5 replicates each treatment. They were fed corn-soybean diets with 0, 0.30, and 0.60 mg of Se/kg from Se yeast and 3.2, 4.0, and 5.4 g of dl-Met/kg, respectively. After incubation, 250 chickens each treatment were randomly divided into 5 replicates and fed the same diet. At 21 d old, 10 male chicks in each treatment were slaughtered. There results were as follows. (1) The Se content significantly increased with the increase of Se yeast supplementation (P < 0.01). (2) The carbonyl content of the myofibrillar protein significantly decreased with the increase of Met supplementation (P < 0.01) and the carbonyl content of the 0 mg of Se/kg treatment was higher than the 0.3 mg of Se/kg treatment (P < 0.01). (3) Selenium supplementation at 0.30 and 0.60 mg/kg significantly decreased malondialdehyde content compared with that of 0 mg of Se/kg (P < 0.01) and 4.0 and 5.4 g of Met/kg supplementation significantly decreased malondialdehyde content compared with that of 3.2 g of Met/kg (P < 0.01). (4) Supplementation of Met at 5.4 g/kg significantly increased International Commission on Illumination a* value compared with 3.2 and 4.0 g of Met/kg (P < 0.01). Supplementation of Se at 0.6 mg/kg significantly increased a* value compared 0 and 0.3 mg of Se/kg (P < 0.01) and 0 mg of Se/kg significantly increased b* value compared with 0.30 and 0.60 mg of Se/kg (P < 0.01). (5) Selenium supplemented at 0.30 and 0.60 mg/kg decreased drip loss compared with 0 mg of Se/kg and 4.0 and 5.4 g of Met/kg decreased drip loss compared with 3.2 g of Met/kg, respectively. The conclusion was drawn that Met and Se yeast supplementation of the maternal diets could improve color, water-holding capacity, and oxidative stability of male offspring meat to an extent.