RESUMEN
The study was conducted to confirm the effects of selenium biofortification on the bioactive compounds and antioxidant activity of wheat microgreen extract. The microgreens were cultivated in the DFT hydroponic system with different concentrations of Se (0 [control], 0.125, 0.25, 0.50, and 1.00â¯mg/L from sodium selenite) in a growth chamber by controlling temperature (25/20⯰C, day/night), light (12â¯h light/dark; intensity 150⯵molâ§m-2â§s-1 with white fluorescence lamp), and humidity (60%) for 10â¯days. Se biofortification increased the germination rate and decreased microgreen length and yield. Chlorophyll and carotenoid levels increased in the Se-biofortified microgreen extract. Bioactive compounds such as phenolics, flavonoids, vitamin C, and anthocyanin significantly increased in 0.25-0.50â¯mg/L of Se-biofortified microgreen extracts. Antioxidant (ABTS, DPPH, NSA and SOD-like) activity also increased at moderate levels (0.25-0.50â¯mg/L) of Se biofortification. Therefore, Se biofortification may be useful for the industrial manufacture of new products from wheat microgreen extract.
Asunto(s)
Antioxidantes/química , Extractos Vegetales/química , Triticum/química , Ácido Ascórbico/análisis , Biofortificación/métodos , Carotenoides/análisis , Clorofila/análisis , Flavonoides/análisis , Germinación/efectos de los fármacos , Fenoles/análisis , Selenito de Sodio/farmacología , Espectrofotometría Ultravioleta , Triticum/crecimiento & desarrollo , Triticum/metabolismo , Regulación hacia Arriba/efectos de los fármacosRESUMEN
This study was conducted to confirm the effects of salinity stress on bioactive compounds and antioxidant activity of wheat microgreen extract. The microgreens were cultivated for 8â¯days in organic media with different concentrations of Na [0 (control), 12.5, 25, 50, and 100â¯mM from sodium chloride] which was contained in a growth chamber with controlled temperature (20/15⯰C, day/night), light (14/10â¯h, light/dark; intensity 150⯵mol·m-2·s-1 with quantum dot light-emitting diodes), and humidity (60%). Treatment with increasing concentrations of Na resulted in an increase in the Na content of microgreens. Treatment with 12.5â¯mM of NaCl significantly maximized ß-carotene (1.21⯵g/mL), phenolic acid (41.70⯵g/mL), flavonoid (165.47⯵g/mL), and vitamin C (29.51⯵g/mL) levels and the nitrite-scavenging activities (37.33%) in wheat microgreen extracts. In addition, the salt-stress caused due to treatment with 25â¯mM of NaCl resulted in the highest anthocyanin (51.43⯵g/mL), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (89.31%), and 2,2-diphenyl-1-picrylhydrazyl (63.28%) radical-scavenging activity. Therefore, attaining adequate levels of salt-stress may be useful for the industrial manufacturing of new products from wheat microgreen extract.