RESUMEN
OBJECTIVE: Cheongseoikki-tang (CIT, Korean), also called Qingshu Yiqi decoction () and Seisho-ekki-to (Japanese), is well known as an effective traditional combination of herbs for treating cardiovascular diseases. This study was to research its effects on bone marrow-derived mast cell (BMMC)-mediated allergy and inflammation mechanisms. METHODS: In this study, the biological effect of Cheongseoikki-tang ethanol extract (CITE) was evaluated, focusing on its effects on the production of allergic mediators by phorbol 12-myristate 13-acetate (PMA) plus calcium ionophore A23187 (A23187)-stimulated BMMCs. These allergic mediators included interleukin-6 (IL-6), prostaglandin D2 (PGD2), leukotriene C4 (LTC4), and ß-hexosaminidase (ß-hex). RESULTS: Our data revealed that CITE inhibited the production of IL-6, PGD2, LTC4, and ß-hex induced by PMA plus A23187 (P<0.05). CONCLUSION: These findings indicate that CITE has the potential for use in the treatment of allergy.
Asunto(s)
Antiinflamatorios/uso terapéutico , Células de la Médula Ósea/patología , Medicamentos Herbarios Chinos/uso terapéutico , Hipersensibilidad/tratamiento farmacológico , Mastocitos/patología , Animales , Antiinflamatorios/farmacología , Calcimicina/farmacología , Degranulación de la Célula/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Medicamentos Herbarios Chinos/farmacología , Hipersensibilidad/patología , Interleucina-6/metabolismo , Leucotrieno C4/farmacología , Masculino , Mastocitos/efectos de los fármacos , Mastocitos/fisiología , Ratones , Ratones Endogámicos BALB C , Prostaglandina D2/biosíntesis , Acetato de Tetradecanoilforbol/farmacología , beta-N-Acetilhexosaminidasas/metabolismoRESUMEN
Tetrandrine (TET) is a bis-benzylisoquinoline alkaloid derived from the radix of Stephania tetrandra S. Moore. TET performs a wide spectrum of biological activities. The radix of S. tetrandrae has been used traditionally in Asia, including Korea, to treat congestive circulatory disorders and inflammatory diseases. The aim of this study was to examine the mechanism of antibacterial activity of tetrandrine against Staphylococcus aureus. The mechanism was investigated by studying the effects of TET in combination with detergent or membrane potential un-couplers. In addition, the direct involvement of peptidoglycan (PGN) was assessed in titration assays. TET activity against S. aureus was 125-250 µg/mL, and the minimum inhibitory concentration (MIC) of the two reference strains was 250 µg/mL. The OD(600) of each suspension treated with a combination of ethylenediaminetetraacetic acid (EDTA), tris(hydroxymethyl) aminomethane (TRIS), and Triton X-100 (TX) with TET (0.25×MIC) had been reduced from 43% to 96%. Additional structure-function studies on the antibacterial activity of TET in combination with other agents may lead to the discovery of more effective antibacterial agents.