Evaluation of physical-mechanical properties, antibacterial effect, and cytotoxicity of temporary restorative materials.

The objective of this study was to compare selective physical-mechanical properties, antibacterial effects and cytotoxicity of seven temporary restorative materials (TRM): five resin-based materials [Bioplic (B), Fill Magic Tempo (FM), Fermit inlay (F), Luxatemp LC (L) and Revotek LC (R)], and zinc oxide-eugenol cement (IRM) and glass ionomer cement (GIC) as the controls. Material and methods The physical-mechanical properties were evaluated by determining microleakage (ML), ultimate tensile strength (UTS) and Shore D hardness (SDH). In addition, the polymerization rate (Pr-1), depth of cure (DC), water sorption and solubility (WS/SL) were evaluated. The antimicrobial effects of the materials were assessed by biofilm accumulation of Streptococcus mutans (BT) and the direct contact test (DCT) by exposure to Enterococcus faecalis for 1 and 24 h, and cytotoxicity by MTT assay. The data were analyzed by ANOVA or Kruskall-Wallis tests, and a complementary post-hoc method (p<0.05). Results Group B, followed by FM and GIC had significantly lower percentages of microleakage in comparison with the other groups; Groups FM and L showed the highest WS, while Groups R and FM showed the significantly lowest SL values (p<0.05). Group R showed the statistically highest UTS mean and the lowest DC mean among all groups. Group F showed the lowest S. mutans biofilm accumulation (p=0.023). Only the Group L showed continued effect against E. faecalis after 1 h and 24 h in DCT. The L showed statistically lower viability cell when compared to the other groups. Conclusions These findings suggest the antibacterial effect of the temporary materials Fill Magic and Bioplic against S. mutans, while Luxatemp showed in vitro inhibition of S. mutans biofilm accumulation and E. faecalis growth. Regarding the cell viability test, Luxatemp was the most cytotoxic and Fill Magic was shown to be the least cytotoxic.

Evaluation of physical-mechanical properties, antibacterial effect, and cytotoxicity of temporary restorative materials

Abstract The objective of this study was to compare selective physical-mechanical properties, antibacterial effects and cytotoxicity of seven temporary restorative materials (TRM): five resin-based materials [Bioplic (B), Fill Magic Tempo (FM), Fermit inlay (F), Luxatemp LC (L) and Revotek LC (R)], and zinc oxide-eugenol cement (IRM) and glass ionomer cement (GIC) as the controls. Material and methods The physical-mechanical properties were evaluated by determining microleakage (ML), ultimate tensile strength (UTS) and Shore D hardness (SDH). In addition, the polymerization rate (Pr-1), depth of cure (DC), water sorption and solubility (WS/SL) were evaluated. The antimicrobial effects of the materials were assessed by biofilm accumulation of Streptococcus mutans (BT) and the direct contact test (DCT) by exposure to Enterococcus faecalis for 1 and 24 h, and cytotoxicity by MTT assay. The data were analyzed by ANOVA or Kruskall-Wallis tests, and a complementary post-hoc method (p<0.05). Results Group B, followed by FM and GIC had significantly lower percentages of microleakage in comparison with the other groups; Groups FM and L showed the highest WS, while Groups R and FM showed the significantly lowest SL values (p<0.05). Group R showed the statistically highest UTS mean and the lowest DC mean among all groups. Group F showed the lowest S. mutans biofilm accumulation (p=0.023). Only the Group L showed continued effect against E. faecalis after 1 h and 24 h in DCT. The L showed statistically lower viability cell when compared to the other groups. Conclusions These findings suggest the antibacterial effect of the temporary materials Fill Magic and Bioplic against S. mutans, while Luxatemp showed in vitro inhibition of S. mutans biofilm accumulation and E. faecalis growth. Regarding the cell viability test, Luxatemp was the most cytotoxic and Fill Magic was shown to be the least cytotoxic.

In situ evaluation of color stability and hardness' decrease of resin-based composites.

OBJECTIVE: To determine the color stability (CS) and hardness' decrease (HD) of two commercial resin-based composites (RBCs), after in situ staining conditions (SC). METHODS: Six disc-shaped specimens (6 × 2 mm) of two RBCs-Filtek Z350 XT (FZX) and IPS Empress Direct (IED)-were randomly distributed into palatal devices. Eight volunteers (aged 20-30 years) participated in this study. The in situ SC consisted of 400 mL consumption (2 × 200 mL/day) of water, orange juice, cola soda, and coffee and 140 mL consumption of red wine, 10 days each. CS was obtained by CIELAB color difference (ΔE*) and HD (in %) by the Knoop method (50 g for 15 s), after curing (baseline) and after the in situ SC. Data were statistically analyzed at α = 0.05 significance level. RESULTS: For both RBCs, the coffee (5.7 ± 0.5 for FZX; 5.9 ± 1.3 for IED) and red wine (6.1 ± 1.1 for FZX; 6.2 ± 1.6 for IED) groups showed the highest ΔE* while water the lowest (1.6 ± 0.6 for FZX; 1.8 ± 0.2 for IED). The highest HD was induced by red wine group for FZX (40.7 ± 5.4) and by cola soda for IED (40.6 ± 3.2) while the water groups (8.3 ± 1.7 for FZX; 7.8 ± 2.1 for IED) presented the lowest values for both RBCs. CONCLUSION: Both RBCs were affected by the in situ SC, but no significant differences for CS and HD were observed between them. Red wine and coffee showed higher discoloration potential. CLINICAL SIGNIFICANCE: This in situ study suggests that color stability and hardness' decrease of resin-based composites were dependent on the type of consumed beverage and materials' composition. Patients with esthetic direct restorations should be aware of the degradative potential of them.