Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 6 de 6
Filtrar
1.
Am J Physiol Endocrinol Metab ; 321(5): E621-E635, 2021 11 01.
Artículo en Inglés | MEDLINE | ID: mdl-34569272

RESUMEN

Amino acids are involved in energy homeostasis, just as are carbohydrates and lipids. Therefore, mechanisms controlling protein intake should operate independently and in combination with systems controlling overall energy intake to coordinate appropriate metabolic and behavioral responses. The objective of this study was to quantify the respective roles of dietary protein and carbohydrate levels on energy balance, plasma fibroblast growth factor 21 (FGF21) and insulin growth factor 1 (IGF-1) concentrations, and hypothalamic neurotransmitters (POMC, NPY, AgRP, and CART). In a simplified geometric framework, 7-wk-old male Wistar rats were fed 12 diets containing 3%-30% protein for 3 wk, in which carbohydrates accounted for 30%-75% of the carbohydrate and fat part of the diet. As a result of this study, most of the studied parameters (body composition, energy expenditure, plasma FGF21 and IGF-1 concentrations, and Pomc/Agrp ratio) responded mainly to the protein content and to a lesser extent to the carbohydrate content in the diet.NEW & NOTEWORTHY As mechanisms controlling protein intake can operate independently and in combination with those controlling energy intakes, we investigated the metabolic and behavioral effects of the protein-carbohydrate interaction. With a simplified geometric framework, we showed that body composition, energy balance, plasma FGF21 and IGF-1 concentrations, and hypothalamic Pomc/Agrp ratio were primarily responsive to protein content and, to a lesser extent, to carbohydrate content of the diet.


Asunto(s)
Carbohidratos de la Dieta/farmacología , Proteínas en la Dieta/farmacología , Metabolismo Energético/fisiología , Factores de Crecimiento de Fibroblastos/biosíntesis , Hipotálamo/fisiología , Proteína Relacionada con Agouti/metabolismo , Animales , Composición Corporal/efectos de los fármacos , Expresión Génica , Factor I del Crecimiento Similar a la Insulina/biosíntesis , Factor I del Crecimiento Similar a la Insulina/genética , Masculino , Neurotransmisores/metabolismo , Proopiomelanocortina/metabolismo , Ratas , Ratas Wistar
2.
Sci Rep ; 11(1): 12436, 2021 06 14.
Artículo en Inglés | MEDLINE | ID: mdl-34127689

RESUMEN

To study, in young growing rats, the consequences of different levels of dietary protein deficiency on food intake, body weight, body composition, and energy balance and to assess the role of FGF21 in the adaptation to a low protein diet. Thirty-six weanling rats were fed diets containing 3%, 5%, 8%, 12%, 15% and 20% protein for three weeks. Body weight, food intake, energy expenditure and metabolic parameters were followed throughout this period. The very low-protein diets (3% and 5%) induced a large decrease in body weight gain and an increase in energy intake relative to body mass. No gain in fat mass was observed because energy expenditure increased in proportion to energy intake. As expected, Fgf21 expression in the liver and plasma FGF21 increased with low-protein diets, but Fgf21 expression in the hypothalamus decreased. Under low protein diets (3% and 5%), the increase in liver Fgf21 and the decrease of Fgf21 in the hypothalamus induced an increase in energy expenditure and the decrease in the satiety signal responsible for hyperphagia. Our results highlight that when dietary protein decreases below 8%, the liver detects the low protein diet and responds by activating synthesis and secretion of FGF21 in order to activate an endocrine signal that induces metabolic adaptation. The hypothalamus, in comparison, responds to protein deficiency when dietary protein decreases below 5%.


Asunto(s)
Dieta con Restricción de Proteínas/efectos adversos , Factores de Crecimiento de Fibroblastos/metabolismo , Hipotálamo/metabolismo , Hígado/metabolismo , Deficiencia de Proteína/metabolismo , Animales , Modelos Animales de Enfermedad , Ingestión de Energía , Metabolismo Energético , Factores de Crecimiento de Fibroblastos/sangre , Humanos , Masculino , Deficiencia de Proteína/sangre , Ratas , Respuesta de Saciedad
3.
Am J Physiol Regul Integr Comp Physiol ; 316(5): R486-R501, 2019 05 01.
Artículo en Inglés | MEDLINE | ID: mdl-30735436

RESUMEN

Low-protein diets most often induce increased energy intake in an attempt to increase protein intake to meet protein needs with a risk of accumulation as fat of the excess energy intake. In female adult BALB/c mice, a decrease in dietary casein from 20% to 6% and 3% increased energy intake and slightly increased adiposity, and this response was exacerbated with soy proteins with low methionine content. The effect on fat mass was however limited because total energy expenditure increased to the same extent as energy intake. Lean body mass was preserved in all 6% fed mice and reduced only in 3% casein-fed animals. Insulin response to an oral glucose tolerance test was reduced in soy-fed mice and in low-protein-fed mice. Low-protein diets did not affect uncoupling protein 1 and increased fibroblast growth factor 21 (FGF21) in brown adipose tissue and increased FGF21, fatty acid synthase, and cluster of differentiation 36 in the liver. In the hypothalamus, neuropeptide Y was increased and proopiomelanocortin was decreased only in 3% casein-fed mice. In plasma, when protein was decreased, insulin-like growth factor-1 decreased and FGF21 increased and plasma FGF21 was best described by using a combination of dietary protein level, protein-to-carbohydrate ratio, and protein-to-methionine ratio in the diet. In conclusion, reducing dietary protein and protein quality increases energy intake but also energy expenditure resulting in an only slight increase in adiposity. In this process, FGF21 is probably an important signal that responds to a complex combination of protein restriction, protein quality, and carbohydrate content of the diet.


Asunto(s)
Adiposidad , Dieta con Restricción de Proteínas , Carbohidratos de la Dieta/administración & dosificación , Ingestión de Energía , Metabolismo Energético , Factores de Crecimiento de Fibroblastos/sangre , Factor I del Crecimiento Similar a la Insulina/metabolismo , Metionina/deficiencia , Valor Nutritivo , Almidón/administración & dosificación , Tejido Adiposo/metabolismo , Alimentación Animal , Fenómenos Fisiológicos Nutricionales de los Animales , Animales , Biomarcadores , Carbohidratos de la Dieta/metabolismo , Regulación hacia Abajo , Femenino , Hipotálamo/metabolismo , Hígado/metabolismo , Ratones Endogámicos BALB C , Almidón/metabolismo , Regulación hacia Arriba
4.
Br J Nutr ; 114(8): 1132-42, 2015 Oct 28.
Artículo en Inglés | MEDLINE | ID: mdl-26285832

RESUMEN

High-protein diets are known to reduce adiposity in the context of high carbohydrate and Western diets. However, few studies have investigated the specific high-protein effect on lipogenesis induced by a high-sucrose (HS) diet or fat deposition induced by high-fat feeding. We aimed to determine the effects of high protein intake on the development of fat deposition and partitioning in response to high-fat and/or HS feeding. A total of thirty adult male Wistar rats were assigned to one of the six dietary regimens with low and high protein, sucrose and fat contents for 5 weeks. Body weight (BW) and food intake were measured weekly. Oral glucose tolerance tests and meal tolerance tests were performed after 4th and 5th weeks of the regimen, respectively. At the end of the study, the rats were killed 2 h after ingestion of a calibrated meal. Blood, tissues and organs were collected for analysis of circulating metabolites and hormones, body composition and mRNA expression in the liver and adipose tissues. No changes were observed in cumulative energy intake and BW gain after 5 weeks of dietary treatment. However, high-protein diets reduced by 20 % the adiposity gain induced by HS and high-sucrose high-fat (HS-HF) diets. Gene expression and transcriptomic analysis suggested that high protein intake reduced liver capacity for lipogenesis by reducing mRNA expressions of fatty acid synthase (fasn), acetyl-CoA carboxylase a and b (Acaca and Acacb) and sterol regulatory element binding transcription factor 1c (Srebf-1c). Moreover, ketogenesis, as indicated by plasma ß-hydroxybutyrate levels, was higher in HS-HF-fed mice that were also fed high protein levels. Taken together, these results suggest that high-protein diets may reduce adiposity by inhibiting lipogenesis and stimulating ketogenesis in the liver.


Asunto(s)
Tejido Adiposo/metabolismo , Dieta Alta en Grasa/efectos adversos , Proteínas en la Dieta/administración & dosificación , Sacarosa en la Dieta/efectos adversos , Lipogénesis , Ácido 3-Hidroxibutírico/sangre , Acetil-CoA Carboxilasa/genética , Acetil-CoA Carboxilasa/metabolismo , Adiposidad , Animales , Glucemia/metabolismo , Composición Corporal , Peso Corporal , HDL-Colesterol/sangre , LDL-Colesterol/sangre , Grasas de la Dieta/administración & dosificación , Sacarosa en la Dieta/administración & dosificación , Ingestión de Energía , Ácido Graso Sintasas/genética , Ácido Graso Sintasas/metabolismo , Ghrelina/sangre , Prueba de Tolerancia a la Glucosa , Hipotálamo/metabolismo , Leptina/sangre , Hígado/metabolismo , Masculino , Músculo Esquelético/metabolismo , Ratas , Ratas Wistar , Proteína 1 de Unión a los Elementos Reguladores de Esteroles/genética , Proteína 1 de Unión a los Elementos Reguladores de Esteroles/metabolismo , Triglicéridos/sangre
5.
Appetite ; 90: 136-43, 2015 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-25772196

RESUMEN

Studies have reported a better satiating effect of eggs when compared with common cereal-based breakfasts, an effect that can be attributed to their macronutrient composition. Our aim was to compare the satiating power of an omelette and cottage cheese, both being common food snacks with similar nutrient compositions (containing proteins and lipids) but in different food forms. Thirty healthy volunteers participated in a randomized crossover trial. On each test day, the subjects consumed one of the two snacks, both providing 1346 kJ, 26 g protein, 21 g lipids, and 8 g lactose. The elapsed time between the snack and lunch request, their food intake at lunch, and their satiety scores were recorded. In a subgroup of 10 volunteers, blood was sampled to measure plasma metabolites and hormones. The two preloads were similar in terms of the time between the snack and a request for the buffet (167 ± 8 min), energy intake at the buffet (3988 ± 180 kJ) and appetite ratings. Plasma amino acid and urea concentrations indicated a marked delay in kinetic delivery after the eggs compared with the cottage cheese. In contrast, glucose, triglycerides and cholesterol displayed similar profiles after the snack. GIP and insulin secretions increased significantly after the cottage cheese, while glucagon and GLP-1 secretions were delayed with the omelette. We conclude that despite important differences in protein kinetics and their subsequent effects on hormone secretion, eggs and cottage cheese had a similar satiating power. This strongly suggests that with dose of proteins that is compatible to supplement strategies, i.e. 20-30 g, a modulation of protein kinetics is ineffective in increasing satiety.


Asunto(s)
Apetito/fisiología , Queso , Óvulo , Saciedad/fisiología , Adulto , Aminoácidos/sangre , Glucemia/análisis , Colesterol/sangre , Estudios Cruzados , Citocinas/sangre , Ingestión de Alimentos/fisiología , Ingestión de Energía/fisiología , Femenino , Voluntarios Sanos , Humanos , Insulina/sangre , Cinética , Masculino , Periodo Posprandial/fisiología , Triglicéridos/sangre , Urea/sangre , Adulto Joven
6.
Amino Acids ; 40(5): 1461-72, 2011 May.
Artículo en Inglés | MEDLINE | ID: mdl-20890620

RESUMEN

The aim of this study was to determine the contribution of dietary amino acids (AA) to energy metabolism under high protein (HP) diets, using a double tracer method to follow simultaneously the metabolic fate of α-amino groups and carbon skeletons. Sixty-seven male Wistar rats were fed a normal (NP) or HP diet for 14 days. Fifteen of them were equipped with a permanent catheter. On day 15, after fasting overnight, they received a 4-g meal extrinsically labeled with a mixture of 20 U-[(15)N]-[(13)C] AA. Energy metabolism, dietary AA deamination and oxidation and their transfer to plasma glucose were measured kinetically for 4 h in the catheterized rats. The transfer of dietary AA to liver glycogen was determined at 4 h. The digestive kinetics of dietary AA, their transfer into liver AA and proteins and the liver glycogen content were measured in the 52 other rats that were killed sequentially hourly over a 4-h period. [(15)N] and [(13)C] kinetics in the splanchnic protein pools were perfectly similar. Deamination increased fivefold in HP rats compared to NP rats. In the latter, all deaminated AA were oxidized. In HP rats, the oxidation rate was slower than deamination, so that half of the deaminated AA was non-oxidized within 4 h. Non-oxidized carbon skeletons were poorly sequestrated in glycogen, although there was a significant postprandial production of hepatic glycogen. Our results strongly suggest that excess dietary AA-derived carbon skeletons above the ATP production capacity, are temporarily retained in intermediate metabolic pools until the oxidative capacities of the liver are no longer overwhelmed by an excess of substrates.


Asunto(s)
Aminoácidos/administración & dosificación , Dieta , Proteínas en la Dieta/administración & dosificación , Suplementos Dietéticos , Ingestión de Energía , Periodo Posprandial , Adenosina Trifosfato/biosíntesis , Animales , Desaminación , Descarboxilación , Metabolismo Energético , Cinética , Hígado/metabolismo , Masculino , Ratas , Ratas Wistar
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA