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1.
Blood ; 103(1): 128-32, 2004 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-12958075

RESUMEN

Fanconi anemia (FA) is an autosomal recessive cancer susceptibility syndrome characterized by cellular sensitivity to genotoxic agents. In recent years, FA proteins have been associated with different molecules involved in signal transduction, which has raised the interest in FA-dependent signaling pathways. Here, we report that the c-Jun N-terminal kinase (JNK) fails to phosphorylate in response to UV radiation and treatment with mitomycin C in FA lymphoblast cells derived from type A patients (FA-A). Furthermore, defective kinase activity seems to be specific for JNK, because extracellular signal-regulated kinase (ERK) responded to the proper stimuli in FA-A cells. We also demonstrate that the early growth-response factor-1 (Egr-1), a JNK downstream target gene that is normally induced by genotoxic stress, is not upregulated in UV-treated FA-A cells. Moreover, FA-A cells are more sensitive to apoptosis than control lymphoblasts. Both JNK and Egr-1 may be part of a pathway triggered by FA proteins, because functional correction of FA-A cells by gene transfer restores, at least in part, JNK activation and Egr-1 expression after UV exposure. Together, our data suggest that activation of JNK and expression of Egr-1 gene in B lymphoblasts mediate a cellular response to genotoxic agents that may be induced by FA proteins.


Asunto(s)
Proteínas de Unión al ADN/genética , Anemia de Fanconi/genética , Anemia de Fanconi/metabolismo , Proteínas Inmediatas-Precoces , Factores de Transcripción/genética , Linfocitos B/metabolismo , Linfocitos B/efectos de la radiación , Secuencia de Bases , Línea Celular , ADN Complementario/genética , Regulación hacia Abajo , Proteína 1 de la Respuesta de Crecimiento Precoz , Activación Enzimática/efectos de la radiación , Proteína del Grupo de Complementación A de la Anemia de Fanconi , Prueba de Complementación Genética , Humanos , Proteínas/genética , Proteínas/metabolismo , Rayos Ultravioleta
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