RESUMEN
Continuing our search for vitamin D analogues, we explored the modification of the steroidal side chain and inserted a methylene moiety in position C-22 together with either lengthening the side chain or introducing a ring at the terminal end. Our conformational studies confirmed that the presence of a methylene group attached to C-22 restricts the conformational flexibility of the side chain, which can result in changes in biological characteristics of a molecule. All synthesized 1α,25-dihydroxy-2,22-dimethylene-19-norvitamin D3 analogues proved equal to calcitriol in their ability to bind to the vitamin D receptor, and most of them exert significantly higher differentiation and transcriptional activity than calcitriol. The most active compounds were characterized by the presence of an elongated side chain or 26,27-dimethylene bridge. The synthetic strategy was based on the Wittig-Horner coupling of the known A-ring phosphine oxide with the corresponding Grundmann ketones prepared from a 20-epi-Inhoffen-Lythgoe diol derived from vitamin D2.
Asunto(s)
Calcitriol/análogos & derivados , Calcio/metabolismo , Animales , Unión Competitiva , Huesos/efectos de los fármacos , Huesos/metabolismo , Calcitriol/farmacología , Técnicas de Química Sintética , Relación Dosis-Respuesta a Droga , Evaluación Preclínica de Medicamentos/métodos , Regulación de la Expresión Génica/efectos de los fármacos , Células HL-60 , Humanos , Intestinos/efectos de los fármacos , Luciferasas/genética , Masculino , Conformación Molecular , Ratas Sprague-Dawley , Receptores de Calcitriol/genética , Receptores de Calcitriol/metabolismo , Relación Estructura-Actividad , Transcripción GenéticaRESUMEN
Vitamin D and sunlight have each been reported to protect against the development of experimental autoimmune encephalomyelitis (EAE), a mouse model of multiple sclerosis (MS). To date, the contribution of each has been unclear as ultra violet (UV) exposure also causes the generation of vitamin D in the skin. To examine whether the UV based suppression of EAE results, at least, in part from the production of vitamin D, we studied the effect of UV light on EAE in mice unable to produce 7-dehydroxycholesterol (7-DHC), the required precursor of vitamin D. Furthermore, we examined UV suppression of EAE in mice devoid of the vitamin D receptor (VDR). Our results demonstrate that UV light suppression of EAE occurs in the absence of vitamin D production and in the absence of VDR. Future investigations will focus on identifying the pathway responsible for the protective action of UV in EAE and presumably human MS.
Asunto(s)
Encefalomielitis Autoinmune Experimental/metabolismo , Encefalomielitis Autoinmune Experimental/terapia , Esclerosis Múltiple/terapia , Fototerapia , Receptores de Calcitriol/metabolismo , Vitamina D/metabolismo , Animales , Colecalciferol/análogos & derivados , Colecalciferol/metabolismo , Modelos Animales de Enfermedad , Encefalomielitis Autoinmune Experimental/genética , Femenino , Humanos , Masculino , Ratones , Ratones Endogámicos C57BL , Esclerosis Múltiple/genética , Esclerosis Múltiple/metabolismo , Receptores de Calcitriol/genética , Piel/metabolismo , Piel/efectos de la radiación , Rayos UltravioletaRESUMEN
Human studies have shown that individuals with colon cancer tend to have lower serum 25-hydroxy-vitamin D3 [25(OH)D3] levels compared with healthy controls, but whether this link is causative, a result of the disease or an indicator of another factor altogether has yet to be demonstrated. In humans, vitamin D, calcium and UV exposure are inextricably linked; therefore, understanding the individual and combined roles of each of these will require animal models specifically designed to address these questions. To begin to untangle this network, our group has employed the ApcPirc/+ rat, which contains a truncating mutation in the Apc gene, leading to the development of colonic tumors. Our group previously utilized this model to demonstrate that vitamin D supplementation above normal does not reduce colonic tumor burden and, in fact, increased tumor multiplicity in a dose-dependent manner. In the current study, we tested whether vitamin D deficiency plays a causative role in tumor development using two strains which differ in their susceptibility to intestinal tumorigenesis. In the colon, vitamin D deficiency did not increase the development of tumors in either strain, and was actually protective in one strain. Unexpectedly, low dietary calcium combined with vitamin D deficiency significantly suppressed tumor development in the small intestine and colon of both strains. The vast majority of tumors in the human intestine occur in the colon, and we find no evidence to support a direct role of vitamin D deficiency in increasing colonic tumorigenesis, and low calcium might protect against tumor development.This article has an associated First Person interview with the first author of the paper.
Asunto(s)
Proteína de la Poliposis Adenomatosa del Colon/genética , Calcio de la Dieta/uso terapéutico , Carcinogénesis/patología , Neoplasias del Colon/patología , Neoplasias del Colon/prevención & control , Progresión de la Enfermedad , Sustancias Protectoras/uso terapéutico , Deficiencia de Vitamina D/complicaciones , Proteína de la Poliposis Adenomatosa del Colon/metabolismo , Animales , Calcitriol/sangre , Calcio/sangre , Calcio de la Dieta/farmacología , Carcinogénesis/efectos de los fármacos , Carcinogénesis/genética , Proliferación Celular/efectos de los fármacos , Proliferación Celular/genética , Neoplasias del Colon/genética , Sulfato de Dextran , Modelos Animales de Enfermedad , Regulación Neoplásica de la Expresión Génica/efectos de los fármacos , Antígeno Ki-67/genética , Antígeno Ki-67/metabolismo , Límite de Detección , Sustancias Protectoras/farmacología , Ratas , Aumento de Peso/efectos de los fármacosRESUMEN
BACKGROUND: Vitamin D analogs and calcimimetics are used to manage secondary hyperparathyroidism (SHPT) in dialysis patients. DP001 is an oral vitamin D analog that suppresses parathyroid hormone (PTH) in uremic rats, osteopenic women, and hemodialysis patients. The safety and effectiveness of DP001 suppressing PTH in dialysis patients previously managed with active vitamin D with or without a calcimimetic are presented. METHODS: A multicenter, randomized, double-blind study compared DP001 to placebo in hemodialysis patients with serum-intact PTH (iPTH) ≥300 pg/ml. The primary efficacy endpoint was the proportion of patients achieving 2 consecutive ≥30% decreases in iPTH levels during the 12 weeks of treatment. Calcium, phosphorus, calcium × phosphorus product and safety were also evaluated. The responses to DP001 were compared in patients previously treated with both active vitamin D and a calcimimetic to those previously on active vitamin D alone. RESULTS: Sixty-two patients were randomized (n = 34 DP001; n = 28 placebo). At week 12, 78% of all DP001-treated patients and 7% of all placebo-treated patients achieved the primary endpoint (p < 0.0001); iPTH fell 45% in the DP001 group and increased 37% in the placebo group. No patient exceeded the safety threshold of 2 consecutively corrected serum calcium levels ≥11.0 mg/dl. Patients previously on cinacalcet plus active vitamin D also responded to DP001 (n = 10) resulting in a 55% decrease in iPTH, while those on placebo (n = 9) increased by 70%. CONCLUSION: DP001 safely and effectively suppressed iPTH in hemodialysis patients with SHPT that were previously managed with active vitamin D alone or with a calcimimetic (www.clinicaltrials.gov, NCT01922843).
Asunto(s)
Calcitriol/análogos & derivados , Hiperparatiroidismo Secundario/tratamiento farmacológico , Fallo Renal Crónico/terapia , Diálisis Renal , Adulto , Anciano , Calcitriol/uso terapéutico , Calcio/metabolismo , Método Doble Ciego , Femenino , Humanos , Hiperparatiroidismo Secundario/etiología , Hiperparatiroidismo Secundario/metabolismo , Fallo Renal Crónico/complicaciones , Masculino , Persona de Mediana Edad , Hormona Paratiroidea/metabolismo , Fósforo/metabolismo , Resultado del TratamientoRESUMEN
BACKGROUND: Epidemiologic studies in humans have shown associations between greater sunlight exposure, higher serum 25-hydroxycholecalciferol [25(OH)D3] concentrations, and reduced colon cancer risk. However, results from a limited number of vitamin D supplementation trials in humans have not shown a protective effect. OBJECTIVE: We sought to determine whether adding to the diet increasing amounts of either 25(OH)D3, the stable metabolite measured in serum and associated with cancer risk, or cholecalciferol (vitamin D3), the compound commonly used for supplementation in humans, could reduce emergent adenomas (chemoprevention) or decrease the growth of existing adenomas (treatment) in the colons of vitamin D-sufficient rats carrying a truncation mutation of adenomatous polyposis coli (Apc), a model of early intestinal cancer. METHODS: Apc(Pirc/+) rats were supplemented with either vitamin D3 over a range of 4 doses [6-1500 µg/(kg body weight · d)] or with 25(OH)D3 over a range of 6 doses [60-4500 µg/(kg body weight · d)] beginning after weaning. Rats underwent colonoscopy every other week to assess effects on adenoma number and size. At termination (140 d of age), the number of tumors in the small intestine and colon and the size of tumors in the colon were determined, and serum calcium and 25(OH)D3 measurements were obtained. RESULTS: At lower doses (those that did not affect body weight), neither of the vitamin D compounds reduced the number of existing or emergent colonic tumors (P-trend > 0.24). By contrast, supplementation at higher doses (those that caused a suppression in body weight gain) with either 25(OH)D3 or vitamin D3 caused a dose-dependent increase in colonic tumor number in both males and females (P-trend < 0.003). CONCLUSIONS: No evidence for protection against colon tumor development was seen with lower dose supplementation with either cholecalciferol or 25-hydroxycholecalciferol. Thus, the association between sunlight exposure and the incidence of colon cancer may involve factors other than vitamin D concentrations. Alternative hypotheses warrant investigation. Furthermore, this study provides preliminary evidence for the need for caution regarding vitamin D supplementation of humans at higher doses, especially in individuals with sufficient serum 25(OH)D3 concentrations.
Asunto(s)
Adenoma/tratamiento farmacológico , Adenoma/prevención & control , Calcifediol/farmacología , Colecalciferol/farmacología , Neoplasias del Colon/tratamiento farmacológico , Neoplasias del Colon/prevención & control , Animales , Calcifediol/sangre , Calcio de la Dieta/sangre , Colecalciferol/sangre , Colon/efectos de los fármacos , Colon/metabolismo , Dieta , Suplementos Dietéticos , Modelos Animales de Enfermedad , Relación Dosis-Respuesta a Droga , Femenino , Masculino , Ratas , Ratas Endogámicas F344 , Deficiencia de Vitamina D/tratamiento farmacológicoRESUMEN
The 20R- and 20S-isomers of 25-hydroxy-2-methylene-vitamin D3 and 3-desoxy-1α,25-dihydroxy-2-methylene-vitamin D3 have been synthesized. Two alternative synthetic routes were devised for preparation of the required A-ring synthons, starting from the chiral compound derived from the (-)-quinic acid and, alternatively, from the commercially available achiral precursor, monoprotected 1,4-cyclohexanedione. The A-ring dienynes were coupled by the Sonogashira process with the respective C,D-ring fragments, the enol triflates derived from the protected (20R)- or (20S)-25-hydroxy Grundmann ketones. All four compounds possessed significant in vivo activity on bone calcium mobilization and intestinal calcium transport. The presence of a 2-methylene group increased intestinal calcium transport activity of all four analogues above that of the native hormone, 1α,25-dihydroxyvitamin D3. In contrast, bone calcium mobilization was equal to that produced by 1α,25-dihydroxyvitamin D3 in compounds having a (20S)-configuration or diminished to one-tenth that of 1α,25-dihydroxyvitamin D3 in compounds with a (20R)-configuration.
Asunto(s)
Calcifediol/química , Calcio/metabolismo , 25-Hidroxivitamina D3 1-alfa-Hidroxilasa/metabolismo , Animales , Huesos/efectos de los fármacos , Huesos/metabolismo , Calcifediol/farmacología , Calcitriol/análogos & derivados , Calcitriol/química , Técnicas de Química Sintética , Evaluación Preclínica de Medicamentos/métodos , Células HL-60/efectos de los fármacos , Humanos , Mucosa Intestinal/metabolismo , Intestinos/efectos de los fármacos , Masculino , Estructura Molecular , Ratas Sprague-Dawley , Receptores de Calcitriol/metabolismo , Relación Estructura-ActividadRESUMEN
BACKGROUND: The use of 1α-hydroxylated vitamin D therapy to control secondary hyperparathyroidism in renal failure patients has been a success story, culminating with the demonstration of increased life expectancy in patients treated with these compounds. However, hypercalcemic episodes have been a recurrent problem with these therapies and have resulted in the added use of calcium mimetics. Clearly there is good reason to search for improved vitamin D therapy. In our inventory of vitamin D compounds, 2-methylene-19-nor-(20S)-1α,25-dihydroxyvitamin D3 (2MD) surfaced as a potential candidate. This was based on its preferential localization in the parathyroid gland and a clear suppression of serum parathyroid hormone (PTH) levels without a change in serum calcium in a clinical trial in postmenopausal women. METHODS: 2MD has now been tested in the rat 5/6-nephrectomy model of renal failure, and in postmenopausal women to determine if it can suppress serum PTH at doses that do not elevate serum calcium and serum phosphorus concentrations. RESULTS: Daily oral treatment of uremic rats on 2.5 ng/bw/day of 2MD dramatically suppressed PTH without a change in serum calcium or serum phosphorus. Further, PTH was suppressed in postmenopausal women after only 3 daily oral doses of 2MD that continued for 4 weeks with no change in serum calcium or serum phosphorus. CONCLUSION: These results coupled with a pharmacokinetic half-life of ~24 h suggest that 2MD given either daily or at the time of dialysis may be a superior therapy for secondary hyperparathyroidism in chronic renal failure patients.
Asunto(s)
Conservadores de la Densidad Ósea/farmacología , Calcitriol/análogos & derivados , Hormona Paratiroidea/sangre , Posmenopausia/efectos de los fármacos , Animales , Conservadores de la Densidad Ósea/metabolismo , Conservadores de la Densidad Ósea/uso terapéutico , Calcitriol/metabolismo , Calcitriol/farmacología , Calcitriol/uso terapéutico , Calcio/sangre , Cromatografía Líquida de Alta Presión , Método Doble Ciego , Femenino , Humanos , Hiperparatiroidismo Secundario/tratamiento farmacológico , Hiperparatiroidismo Secundario/etiología , Masculino , Glándulas Paratiroides/metabolismo , Fósforo/sangre , Posmenopausia/sangre , Ratas , Insuficiencia Renal Crónica/complicaciones , UremiaRESUMEN
We have recently obtained 1-desoxy and 3-desoxy analogs of (20S)-1α,25-dihydroxy-2-methylene-19-norvitamin D3 (2MD), a compound exerting significantly enhanced calcemic activity and currently being evaluated as a potential drug for osteoporosis. In order to further explore this class of pharmacologically important vitamin D compounds we have decided to synthesize analogs characterized by the presence of two A-ring exocyclic methylene groups attached to C-2 and C-10. The Sonogashira coupling of a triflate enol of the protected (20R)- or (20S)-25-hydroxy Grundmann ketone and the corresponding dienyne A-ring fragment provided the target compounds. A new synthetic path was elaborated, leading to the desired A-ring synthon, that started from commercially available 1,4-cyclohexanedione monoethylene acetal. Biological in vitro and in vivo activities of the synthesized 25-hydroxy-2-methylene-vitamin D3 compounds, belonging to 20R- and 20S-series, were evaluated and discussed. This article is part of a Special Issue entitled 'Vitamin D Workshop'.
Asunto(s)
Calcitriol/análogos & derivados , Animales , Huesos/efectos de los fármacos , Huesos/metabolismo , Calcitriol/síntesis química , Calcitriol/farmacología , Calcio/metabolismo , Diferenciación Celular/efectos de los fármacos , Técnicas de Química Sintética , Evaluación Preclínica de Medicamentos , Células HL-60 , Humanos , Ratas , Receptores de Calcitriol/metabolismo , Estereoisomerismo , Relación Estructura-Actividad , Transcripción Genética/efectos de los fármacosRESUMEN
The steroid hormone 1α,25-dihydroxyvitamin D3 [1α,25-(OH)2D3] is the most active metabolite of vitamin D3 which exerts its control over a multitude of biological processes related to calcium and phosphorus homeostasis, cell proliferation and differentiation, and immune regulation. Unfortunately, the therapeutic application of 1α,25-(OH)2D3 is limited by induction of hypercalcemia. The need for vitamin D compounds with selective biological profiles has stimulated the synthesis of more than three thousand analogs of 1α,25-(OH)2D3. Most of these compounds have structural modifications in the side chain and A-ring; there is also an increasing number of modifications in the CD-rings and limited number in the triene system (seco-B ring). Herein, we report the synthesis and biological evaluation of seco-A-19-nor analogs of 1α,25-dihydroxyvitamin D3, developed to study the role of ring A in the biological activity of 1α,25-(OH)2D3. Interestingly, compounds 2 and 4 show substantial ability to bind the vitamin D receptor and the former is also characterized by selective intestinal calcium transport activity. This article is part of a Special Issue entitled 'Vitamin D Workshop'.
Asunto(s)
Calcitriol/análogos & derivados , Animales , Sitios de Unión , Calcitriol/síntesis química , Calcitriol/farmacología , Calcio/metabolismo , Diferenciación Celular/efectos de los fármacos , Técnicas de Química Sintética , Evaluación Preclínica de Medicamentos , Células HL-60 , Humanos , Modelos Moleculares , Ratas , Receptores de Calcitriol/química , Receptores de Calcitriol/metabolismo , Relación Estructura-Actividad , Transcripción Genética/efectos de los fármacosRESUMEN
We have recently described the synthesis and biological evaluation of 1α,25-dihydroxy-6-methylvitamin D3 which binds the vitamin D receptor (VDR) very effectively. Unfortunately, this compound has a strong tendency to rearrange to its previtamin form. Taking this into consideration, we decided to synthesize a series of 6-substituted analogs of 1α,25-dihydroxy-19-norvitamin D3 which lack the exomethylene moiety at C-10 and thus are unable to undergo a conversion to their respective previtamin forms. The synthesis of analogs bearing different substituents at C-6 was accomplished by Suzuki-Miyaura cross-coupling reaction of a bicyclic organoboron derivative (CD-ring fragment) with the respective alkenyl halides (A-ring synthons). The biological in vitro studies of the affinity of synthesized analogs to the full-length recombinant rat VDR indicate that presence of a bulky, polar substituent at C-6 results in decrease in binding ability. Moreover, introduction of a 6-methyl substituent into the 1α,25-dihydroxy-19-norvitamin D3 molecule results in the 9 times lower affinity of the homolog to the VDR while the same modification of calcitriol has not influenced binding activity. This article is part of a Special Issue entitled 'Vitamin D Workshop'.
Asunto(s)
Calcitriol/análogos & derivados , Animales , Calcitriol/síntesis química , Calcitriol/farmacología , Diferenciación Celular/efectos de los fármacos , Técnicas de Química Sintética , Evaluación Preclínica de Medicamentos , Células HL-60 , Humanos , Ratas , Receptores de Calcitriol/metabolismo , Relación Estructura-Actividad , Transcripción Genética/efectos de los fármacosRESUMEN
9-Alkylidene analogs of 19-nor-1α,25-(OH)2D3 were synthesized, possessing a 'reversed' triene system compared to the natural hormone. The conjugated triene moiety of the novel analogs was constructed by coupling an enyne anion, representing an A-ring synthon, with a 9α-substituted Grundmann ketone derivative. Regioselective dehydration followed by semihydrogenation under Lindlar conditions, provided the desired 9-alkylated 19-norprevitamins which were thermally isomerized to the corresponding 9-methylene and 9-ethylidene analogs of 19-norcalcitriol. It was established that only the former compound had significant binding affinity to the full-length recombinant rat vitamin D receptor. The remaining in vitro studies show very low activity of both analogs. This article is part of a Special Issue entitled 'Vitamin D Workshop'.
Asunto(s)
Calcitriol/análogos & derivados , Animales , Calcitriol/síntesis química , Calcitriol/farmacología , Diferenciación Celular/efectos de los fármacos , Técnicas de Química Sintética , Evaluación Preclínica de Medicamentos , Células HL-60 , Humanos , Ratas , Receptores de Calcitriol/metabolismo , Relación Estructura-Actividad , Transcripción Genética/efectos de los fármacosRESUMEN
Epidemiological studies indicate that sunlight exposure and vitamin D are each associated with a lower risk of colon cancer. The few controlled supplementation trials testing vitamin D in humans reported to date show conflicting results. We have used two genetic models of familial colon cancer, the Apc(Pirc/+) (Pirc) rat and the Apc(Min/+) (Min) mouse, to investigate the effect of 25-hydroxyvitamin D(3) [25(OH)D(3)] and two analogs of vitamin D hormone on colonic tumors. Longitudinal endoscopic monitoring allowed us to test the efficacy of these compounds in preventing newly arising colonic tumors and in affecting established colonic tumors. 25(OH)D(3) and two analogs of vitamin D hormone each failed to reduce tumor multiplicities or alter the growth patterns of colonic tumors in the Pirc rat or the Min mouse.
Asunto(s)
Neoplasias del Colon/prevención & control , Vitamina D/administración & dosificación , Animales , Neoplasias del Colon/metabolismo , Suplementos Dietéticos , Modelos Animales de Enfermedad , Femenino , Masculino , Ratones , Ratas , Vitamina D/metabolismoRESUMEN
During our ongoing structure-activity studies in the vitamin D area, we obtained (20S)-1alpha,25-dihydroxy-2-methylene-19-norvitamin D3 (5). This analog, designated 2MD, is characterized by a significantly enhanced calcemic activity and is currently evaluated as a potential drug for osteoporosis. Therefore, it was of interest to synthesize also its 1-desoxy analog and to evaluate its biological action. These studies were aimed at solving an intriguing problem: can such a vitamin also be hydroxylated in vivo at the allylic C-1 position despite lack of the exomethylene moiety at C-10? The Wittig-Horner coupling of the known protected (20S)-25-hydroxy Grundmann ketone 17 and the phosphine oxides 16 and 33, differing in their hydroxyls protection, provided the target 1-desoxy-2MD (6) after removal of the silyl protecting groups. Two synthetic paths have been elaborated leading to the desired A-ring synthons and starting from commercially available compounds: 1,4-cyclohexanedione monoethylene acetal (7) and (-)-quinic acid (19). The biological activity in vitro of the synthesized 1-desoxy-2MD (6) was evaluated and this analog was found to have an affinity for the vitamin D receptor (VDR) similar as its parent compound 2MD (5) while being much less active in the transcriptional assay. The results of the biological tests in vivo are also discussed.
Asunto(s)
Calcitriol/análogos & derivados , Animales , Unión Competitiva , Calcitriol/síntesis química , Calcitriol/farmacología , Diseño de Fármacos , Evaluación Preclínica de Medicamentos/métodos , Células HL-60 , Humanos , Masculino , Óxidos/química , Fosfinas/química , Ratas , Ratas Sprague-Dawley , Receptores de Calcitriol/química , Proteínas Recombinantes/química , Transcripción GenéticaRESUMEN
UNLABELLED: Vitamin D analogs are being developed that retain therapeutic effects but are less calcemic and phosphatemic, a concern in CKD patients who are prone to vascular calcification. We tested a new analog of vitamin D, 2MbisP, and found that it suppresses PTH at doses that do not affect serum Ca or P. INTRODUCTION: Calcitriol is used for the treatment of secondary hyperparathyroidism. However, its use is often limited by the development of hypercalcemia and hyperphosphatemia, an important consideration in patients with chronic kidney disease (CKD) because they are prone to vascular calcification. To minimize this toxicity, structural modifications in the vitamin D molecule have led to the development of calcitriol analogs with selective actions. MATERIALS AND METHODS: In this study, we compared the effects of 1,25(OH)(2)D(3) and a new analog, 2-methylene-19-nor-(20S)-1 alpha-hydroxy-bishomopregnacalciferol (2MbisP), on the development of secondary hyperparathyroidism and established secondary hyperparathyroidism in uremic rats and on mobilization of calcium and phosphorus from bone in parathyroidectomized rats. The clearance from circulation, half-life, and binding affinities to the vitamin D-binding protein and vitamin D receptor of this compound were also evaluated. RESULTS: Uremia produced a marked rise in plasma PTH, but treatment every other day for 2 wk with either 1,25(OH)(2)D(3) (4 ng) or 2MbisP (250, 750, 1500, or 3000 ng) suppressed this increase by >50%. The suppression by 1,25(OH)(2)D(3), however, was accompanied by increases in ionized calcium, phosphorus, and the calcium x phosphorus product, whereas these three parameters were unchanged by 2MbisP. The binding affinity of 2MbisP was 10-20 times less for the vitamin D receptor and 1000 times less for the serum vitamin D-binding protein compared with 1,25(OH)(2)D(3). Also, 2MbisP was cleared more rapidly from the circulation (t1/2 = 10 min) than 1,25-(OH)(2)D(3) (t1/2=7-9 h). In parathyroidectomized rats fed calcium-or phosphorus-deficient diets, daily injections of 2MbisP (1500 or 3000 ng), unlike 1,25(OH)(2)D(3) (50 ng), had no effect on calcium or phosphorus mobilization from bone. CONCLUSIONS: In uremic rats, 2MbisP can suppress PTH at doses that do not affect plasma calcium, phosphorus, and calcium x phosphorus product. This new vitamin D analog may represent an important tool in the treatment of secondary hyperparathyroidism in patients with CKD.