RESUMEN
INTRODUCTION: A vaccine against hepatitis C has not yet been developed. Recombinant proteins and plasmids encoding hepatitis C virus (HCV) proteins, the components of candidate vaccines, induce a weak immune response and require the use of adjuvants. The aim of the work was to study the adjuvant action of an aqueous solution of fullerene C60 during immunization of mice with HCV recombinant protein NS5B (rNS5B) that is an RNA-dependent RNA polymerase, or with NS5B-encoding pcNS5B plasmid. MATERIALS AND METHODS: An aqueous solution of dispersed fullerene (dnC60) was obtained by ultrafiltration. C57BL/6 mice were immunized with rNS5B subcutaneously, pcNS5B intramuscularly mixed with different doses of dnC60 three times, then the humoral and cellular response to HCV was evaluated. RESULTS: Mice immunization with rNS5B in a mixture with dnC60 at doses of 250 g/mouse significantly induced humoral response: a dose-dependent increase in IgG1 antibody titers was 720 times higher than in the absence of fullerene. There was no increase in the cellular response to rNS5B when administered with dnC60. The humoral response to DNA immunization was weak in mice of all groups receiving pcNS5B. The cellular response was suppressed when the plasmid was injected in a mixture with dnC60. CONCLUSIONS: Dispersed fullerene dnC60 is a promising adjuvant for increasing the immunostimulating activity of weakly immunogenic proteins including surface and other HCV proteins, important for a protective response. Further research is needed to enhance the ability of dnC60 to boost the cellular immune response to the components of the candidate vaccine.
Asunto(s)
Fulerenos , Hepatitis C , Vacunas de ADN , Vacunas contra Hepatitis Viral , Ratones , Animales , Hepacivirus , Fulerenos/farmacología , Fulerenos/metabolismo , Secuencia de Bases , Aminoácidos/genética , Aminoácidos/metabolismo , Aminoácidos/farmacología , Ratones Endogámicos C57BL , Adyuvantes Inmunológicos/genética , Inmunidad Celular , Proteínas Recombinantes/genética , Ratones Endogámicos BALB C , Vacunas de ADN/genética , Vacunas de ADN/farmacología , Vacunas contra Hepatitis Viral/genética , Vacunas contra Hepatitis Viral/farmacologíaRESUMEN
Zinc and vitamin C supplementation of the body is important for CNS functioning. Zinc ions are involved in the neurotransmission (signal transmission from acetylcholine, catecholamine, serotonin, prostaglandin receptors) and in ubiquitin-related protein degradation. Zinc deficits are associated with Alzheimer's disease and depression. Zinc supplementation (10-30 mg daily) improves neurologic recovery rate in patients with stroke and brain injury, has a positive impact on memory and reduces hyperactivity in children. Vitamin C, a zinc synergist, maintains antioxidant resources of the brain, synaptic activity and detoxification. Vitamin C in dose 130-500 mg daily should be used to prevent dementia and neurodegenerative pathology.
Asunto(s)
Antioxidantes/farmacología , Ácido Ascórbico/farmacología , Atención/efectos de los fármacos , Memoria/efectos de los fármacos , Enfermedades del Sistema Nervioso/prevención & control , Vitaminas/administración & dosificación , Zinc/farmacología , Enfermedad de Alzheimer/prevención & control , Antioxidantes/administración & dosificación , Ácido Ascórbico/administración & dosificación , Encéfalo/efectos de los fármacos , Encéfalo/metabolismo , Niño , Suplementos Dietéticos , Sinergismo Farmacológico , Humanos , Enfermedades del Sistema Nervioso/epidemiología , Proteolisis , Riesgo , Ubiquitinación , Vitaminas/farmacología , Zinc/administración & dosificación , Zinc/deficienciaRESUMEN
A novel antiviral drug with immunomodulatory activity (Phosprenyl) is presented. The main active ingredient of the preparation is polyprenyl phosphates. This medicine is highly efficient against a number of viruses, including HIV in vitro, and tick-borne encephalitis and rabies viruses in the experimental models in vivo. In veterinary practice Phosprenyl is now regarded as an effective therapeutic means for treatment of canine distemper, hepatitis, enteritis, etc.
Asunto(s)
Adyuvantes Inmunológicos , Antivirales , Enfermedades de los Perros/tratamiento farmacológico , Fosfatos de Poliisoprenilo , Virosis/veterinaria , Adyuvantes Inmunológicos/química , Adyuvantes Inmunológicos/farmacología , Adyuvantes Inmunológicos/uso terapéutico , Animales , Antivirales/química , Antivirales/farmacología , Antivirales/uso terapéutico , Ensayos Clínicos como Asunto , Cricetinae , Moquillo/tratamiento farmacológico , Perros , Fosfatos de Dolicol/fisiología , Evaluación Preclínica de Medicamentos , Enteritis/tratamiento farmacológico , Enteritis/veterinaria , Hepatitis Viral Animal/tratamiento farmacológico , Ratones , Estructura Molecular , Infecciones por Parvoviridae/tratamiento farmacológico , Infecciones por Parvoviridae/veterinaria , Fosfatos de Poliisoprenilo/química , Fosfatos de Poliisoprenilo/farmacología , Fosfatos de Poliisoprenilo/uso terapéutico , Ratas , Virosis/tratamiento farmacológicoRESUMEN
Viremia accompanying influenza infection and the possibility of transplacental passage of the virus into the fetus make it expedient to develop measures for the prophylaxis of intrauterine infection of the fetus in case of influenza during pregnancy. The work presents the optimum scheme of administration of T-activin for prophylactic purposes to pregnant mice with acute influenza infection. Besides, the use of T-activin for immunocorrection in case of established congenital influenza infection in mice is proposed.