Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Más filtros

Bases de datos
Tipo del documento
Intervalo de año de publicación
1.
Anal Sci ; 37(9): 1253-1258, 2021 Sep 10.
Artículo en Inglés | MEDLINE | ID: mdl-33612559

RESUMEN

The present work studied an acclimation method for phosphorus accumulating organisms (PAOs) with a high content of acetone in culture solutions to develop microbial-based enzyme sensors for highly hydrophobic organophosphorus (OP) pesticides. Through three steps of cultivation and acclimation, only rod-shaped bacteria survived among the various PAOs. The extracellular enzymes released from the acclimated PAOs were salted out by using ammonium sulfate, then purified by a dialysis membrane and a DEAE-Sepharose FF anion exchange column. Two enzyme components were successfully separated-both of which showed hydrolase activity on disodium p-nitrophenyl phosphate (enzyme I, 1.57 µmol/(min·µg); enzyme II, 0.88 µmol/(min·µg) at 45°C). Further, SDS-PAGE gel electrophoresis results showed that the molecular weights of enzymes I and II were about 15.11 and 11.98 kDa, respectively. On this basis, the applicability of the enzyme in hydrophobic OP biosensors was demonstrated.


Asunto(s)
Arildialquilfosfatasa , Fósforo , Aclimatación , Acetona , Electroforesis en Gel de Poliacrilamida , Peso Molecular
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA