RESUMEN
Oral administration of protein can induce antigen-specific immune hyporesponsiveness. However, the utility of oral tolerance to autoantigens in the treatment of autoimmune diseases may be limited when candidate autoantigens cannot be produced by conventional systems in quantities sufficient for clinical studies. Plants may be ideally suited for this purpose, as they can synthesize, glycosylate and assemble mammalian proteins to provide huge quantities of relatively low cost soluble proteins. Furthermore, edible transgenic plants could provide a simple and direct method of autoantigen delivery for oral tolerance. Therefore, the aim of this study was to determine whether a transgenic plant expression system was capable of synthesizing the diabetes-associated autoantigen, glutamic acid decarboxylase (GAD) in an immunogenic form and whether the oral administration of an autoantigen expressed by a plant could directly induce protective immune responses in a mouse model of diabetes. We show that a GAD-expressing transgenic plant, given as a dietary supplement, inhibits the development of diabetes in the non-obese diabetic (NOD) mouse.
Asunto(s)
Autoantígenos/inmunología , Diabetes Mellitus Tipo 1/prevención & control , Glutamato Descarboxilasa/inmunología , Tolerancia Inmunológica , Agrobacterium tumefaciens , Animales , Autoanticuerpos/sangre , Autoantígenos/administración & dosificación , Autoantígenos/genética , Células Cultivadas , Diabetes Mellitus Tipo 1/inmunología , Dieta , Femenino , Vectores Genéticos , Glutamato Descarboxilasa/administración & dosificación , Glutamato Descarboxilasa/genética , Interferón gamma/análisis , Interleucina-10/análisis , Interleucina-4/análisis , Ratones , Ratones Endogámicos NOD , Plantas Modificadas Genéticamente , Plantas Tóxicas , Proteínas Recombinantes de Fusión/administración & dosificación , Proteínas Recombinantes de Fusión/genética , Proteínas Recombinantes de Fusión/inmunología , Solanum tuberosum , Bazo/citología , NicotianaRESUMEN
Tissue tolerance to pelvic intraarterial chemoembolization with cisplatin-lipiodol suspension was studied in rabbits. Cisplatin (3 mg/kg) was used with different doses of lipiodol (0.0, 0.1, 0.2, and 0.3 ml/kg). Cisplatin-lipiodol suspension was injected into the umbilical artery through a long polyethylene catheter. Local tissue concentration of platinum was increased with lipiodol, while that in the liver, heart, and kidneys was reduced. Tissue retention of platinum was linearly related to the dosage of lipiodol. With a single dose of 0.2 ml/kg lipiodol, only slight degeneration and sparse hemorrhage were observed without necrosis.