RESUMEN
BACKGROUND: To study the radioprotective effects of flavonoids from Rosa roxburghii Tratt (FRT). MATERIALS AND METHODS: The radioprotective effects of FRT were investigated by examining cell viability, 30-day survival of mice and the number of colony-forming units in spleen (CFU-S) after total-body 60Co irradiation. RESULTS: The survival rates of irradiated cells gradually increased with increasing concentrations of FRT. The survival rate was the highest at 87% with a concentration of 30 µg/mL. Pretreatment with FRT was needed to realize its radioprotective activity in mice at the dose of 60 mg/kg. With the increasing doses of 30 mg/kg, 60 mg/kg and 120 mg/kg, the numbers of CFU-S increased, and were significantly different compared with the control group. CONCLUSIONS: Pretreatment with FRT prior to irradiation resulted in significantly higher cell survival at 24 h after 5 Gy radiation, increased 30-day survival in mice after exposure to a potentially lethal dose of 8 Gy, and resulted in a higher number of CFU-S in mice after exposure to a dose of 6 Gy. These results collectively indicate that FRT is an effective radioprotective agent.
Asunto(s)
Flavonoides/farmacología , Rayos gamma , Linfocitos/efectos de los fármacos , Extractos Vegetales/farmacología , Protectores contra Radiación/farmacología , Rosa/química , Bazo/efectos de los fármacos , Animales , Ensayo de Unidades Formadoras de Colonias , Flavonoides/química , Linfocitos/efectos de la radiación , Masculino , Ratones , Extractos Vegetales/química , Protectores contra Radiación/química , Bazo/efectos de la radiación , Tasa de Supervivencia , Irradiación Corporal TotalRESUMEN
Our previous study has demonstrated that the hypothalamic supraoptic nucleus (SON) plays a role in pain modulation. Oxytocin (OXT) and arginine vasopressin (AVP) are the important hormones synthesized and secreted by the SON. The experiment was designed to investigate which hormone was relating with the antinociceptive role of the SON in the rat. The results showed that (1) microinjection of L-glutamate sodium into the SON increased OXT and AVP concentrations in the SON perfusion liquid, (2) pain stimulation induces OXT, but not AVP release in the SON, and (3) intraventricular injection (pre-treatment) with OXT antiserum could inhibit the pain threshold increase induced by SON injection of L-glutamate sodium, but administration of AVP antiserum did not influence the antinociceptive role of SON stimulation. The data suggested that the antinociceptive role of the SON relates to OXT rather than AVP.
Asunto(s)
Analgésicos/farmacología , Arginina Vasopresina/metabolismo , Hipotálamo/efectos de los fármacos , Oxitocina/metabolismo , Dolor/metabolismo , Núcleo Supraóptico/efectos de los fármacos , Núcleo Supraóptico/metabolismo , Animales , Ácido Glutámico/farmacología , Inyecciones Intraventriculares , Masculino , Radioinmunoensayo , Ratas , Ratas Sprague-DawleyRESUMEN
OBJECTIVE: To study the chemical constituents of Isodon macrophyllus. METHODS: Compounds were spearated and purified by silica gel column chromatography and their structures were elucidated on the basis of spectral data and chemical evidences. RESULTS: Six compounds were obtained from EtOAc extract of leaves of Isodon macroplhllus and identified as rabdophyllin H(I), lushan-ruhescensins F(II), maovecrystal J(III), Taibaijap onicain A(IV), rabdosinate(V), dancostero(VI). CONCLUSION: Co mpounds II-IV were isolated from this plant for the first time.