Effects of land use on the distribution of soil microplastics in the Lihe River watershed, China.

Soil contamination by microplastics (MPs) has gained widespread attention, whose fate may be influenced by land use types. The effects of land use types and the intensity of human activities on the distribution and sources of soil MPs at the watershed scale are unclear. In this study, 62 surface soil sites in representing five land use types (urban, tea garden, dryland, paddy field and woodland) and 8 freshwater sediment sites were investigated in the Lihe River watershed. MPs were detected in all samples, and the average abundance of soil and sediments was 401.85 ± 214.02 and 222.13 ± 54.66 items/kg, respectively. The soil MPs abundance followed the sequence: urban > paddy field > dryland > tea garden > woodland. Soil MP distribution and MP communities were significant different (p < 0.05) among land use types. The similarity of MP community highly correlated with geographic distance, and woodlands and freshwater sediments may be a potential fate for MPs in the Lihe River watershed. Soil clay, pH, and bulk density significantly correlated with MP abundance and fragment shape (p < 0.05). The positive correlation between population density, Total- Point of Interest (POI) and MP diversity indicates the importance of intensity of human activities in exacerbating soil MP pollution (p < 0.001). Plastic waste sources accounted for 65.12%, 58.60%, 48.15% and 25.35% of MPs in urban, tea garden, dryland and paddy field soils, respectively. Differences in the intensity of agricultural activities and cropping patterns were associated with different percentages of mulching film sources in the three types of agricultural soils. This study provides new ideas for the quantitative analysis of soil MP sources in different land use types.

Dietary fatty acids promote lipid droplet diversity through seipin enrichment in an ER subdomain.

Exogenous metabolites from microbial and dietary origins have profound effects on host metabolism. Here, we report that a sub-population of lipid droplets (LDs), which are conserved organelles for fat storage, is defined by metabolite-modulated targeting of the C. elegans seipin ortholog, SEIP-1. Loss of SEIP-1 function reduces the size of a subset of LDs while over-expression of SEIP-1 has the opposite effect. Ultrastructural analysis reveals SEIP-1 enrichment in an endoplasmic reticulum (ER) subdomain, which co-purifies with LDs. Analyses of C. elegans and bacterial genetic mutants indicate a requirement of polyunsaturated fatty acids (PUFAs) and microbial cyclopropane fatty acids (CFAs) for SEIP-1 enrichment, as confirmed by dietary supplementation experiments. In mammalian cells, heterologously expressed SEIP-1 engages nascent lipid droplets and promotes their subsequent expansion in a conserved manner. Our results suggest that microbial and polyunsaturated fatty acids serve unexpected roles in regulating cellular fat storage by promoting LD diversity.