RESUMEN
In this study, a novel water-soluble polysaccharide (PVLP-1) was extracted and purified from Sacha inchi (Plukenetia volubilis L.) seeds and the structure, antioxidant and immunomodulatory activity of PVLP-1 were investigated. PVLP-1 (144 kDa) consisted of glucose (69.76%), mannose (14.86%), arabinose (10.53%), galactose (2.42%), ribose (1.23%), rhamnose (0.27%) and xylose (0.93%). PVLP-1 displayed characteristic polysaccharide bands in Fourier transform NMR spectra and infrared. The primary structure of PVLP-1 was a heteropolysaccharide with a backbone of (1 â 6)-linked glucose, sidechains of (1 â 4)-linked mannose, (1 â 4)-linked glucose and (1 â 3, 6)-linked mannose and a residue unit of â1)-linked arabinose as revealed the methylation analysis. PVLP-1 possessed good water-holding capacity (WHC), oil-holding capacity (OHC) and antioxidant capacities. Besides, PVLP-1 induced the proliferation of RAW264.7 cell and enhanced the expression of inflammatory cytokines IL-6, TNF-alpha(TNF-α) and IL-1 beta (IL-1ß). The present study indicated that PVLP-1 possessed immune-enhancing bioactivities and could be functional food or adjuvant drug to improve biological immunity of immunodeficiency diseases and hypoimmunity.
Asunto(s)
Adyuvantes Inmunológicos/farmacología , Antioxidantes/farmacología , Proliferación Celular/efectos de los fármacos , Euphorbiaceae/química , Polisacáridos/análisis , Polisacáridos/farmacología , Semillas/química , Animales , Arabinosa/análisis , Supervivencia Celular/efectos de los fármacos , Carbohidratos de la Dieta/metabolismo , Carbohidratos de la Dieta/farmacología , Galactosa/análisis , Glucosa/análisis , Interleucina-1beta/metabolismo , Interleucina-6/metabolismo , Manosa/análisis , Ratones , Fagocitosis/efectos de los fármacos , Aceites de Plantas/química , Polisacáridos/química , Polisacáridos/aislamiento & purificación , Células RAW 264.7 , Ramnosa/análisis , Ribosa/análisis , Espectroscopía Infrarroja por Transformada de Fourier , Factor de Necrosis Tumoral alfa/metabolismo , Agua/química , Xilosa/análisisRESUMEN
In this study, a novel lactic acid probiotic named Bacillus sp. DU-106 was introduced to ferment Dendrobium officinale (D. officinale) polysaccharides, and the effects of such process on the structure and immunostimulatory activity of D. officinale polysaccharide were investigated. Three polysaccharides were subsequently purified from unfermented D. officinale stem (UDP-1), fermented D. officinale stem (FDP-1), and polysaccharide in fermented liquid (FLP-1). After fermentation, the average molecular weight (Mw) of FDP-1 increased from 4.92â¯×â¯105â¯Da (UDP-1) to 5.21â¯×â¯105â¯Da. Fermentation increased the proportions of mannose in FDP-1 by 51.38% compared with that in UDP-1. FDP-1 substantially stimulated cell proliferation and nitric oxide and interleukin-1ß (IL-1ß) production in RAW 264.7 cells. Probiotic fermentation by Bacillus sp. DU-106 could alter monosaccharide composition and Mw and promote immunostimulatory activities of D. officinale polysaccharide, implying the possible application of Bacillus sp. DU-106-fermented D. officinale polysaccharides as auxiliary functional material in immunotherapy.