Role of gamma-oryzanol in drought-tolerant and susceptible cultivars of rice (Oryza sativa L.).

Drought-tolerant cultivars and their phytochemical composition, which has a role in providing drought tolerance are gaining importance. In this study, rice bran oil and semi-purified oryzanol (SPO) obtained from five rice (Oryza sativa L.) cultivars, namely P1401 and PB1 (drought-susceptible) and N22, PNR381 and APO (drought-tolerant) were analyzed for the gamma-oryzanol content, an antioxidant present in considerable amount in the rice bran. The higher level of gamma-oryzanol and its antioxidant activity was observed in drought-tolerant cultivars (N22, PNR381 and APO) as compared to drought-susceptible (PB1 and P1401), suggesting the role of gamma-oryzanol in drought tolerance, as antioxidants are known to play an important role by scavenging free radicals. The total antioxidant activity of gamma-oryzanol might be attributed to 24-methylene cycloartanyl ferulate, a major component of gamma-oryzanol. By enhancing the level of active oryzanol components identified in this study by genetic and molecular means could impart increased drought tolerance.

Characterization and expression of codon optimized soybean phytase gene in E. coli.

Phytic acid, the major storage form of phosphorus in plant seeds is degraded by the phytases to yield inositol and free phosphate, contributing thereby to the improved bioavailability of phytate phosphorus and essential minerals in plant foods and simultaneous reduction in phosphorus pollution of the terrestrial and aquatic ecosystems. As a possible strategy for altering seed phytate levels, the approach involving reduction of phytate content by ectopically expressing endogenous phytase gene during seed development of soybean (Glycine max L. cv. Pusa-20) was attempted in the present study. Semi-quantitative RT-PCR revealed the maximum expression of phytase gene transcripts in germinating cotyledons (approximately 10 days after germinations), compared to other vegetative tissues. A full-length phytase cDNA was amplified from the germinating seedlings by splicing by overlap extension (SOE)-PCR and its sequence analysis revealed an open-reading-frame of 1644 bp, including an N terminal signal peptide of 28 amino acids. Predicted amino acid sequence (547-aa) of molecular mass 62 kDa on alignment with related purple acid phosphatases in other plants shared five conserved domains and seven invariant amino acids involved in coordination of the metals in the binuclear center of purple acid phosphatases. Owing to a large number of E. coli low-usage codons in soybean phytase gene, the modified gene was cloned into a prokaryotic expression vector pET-28a (+) and its expression in E. coli was confirmed by SDS-PAGE and Western blot analysis. Bioassay of the crude expression product in E. coli revealed a functional phytase gene, showing a great potential for developing low phytate transgenic soybean through its seed-specific overexpression in the early stages of seed development.

Polyhydroxyalkanoate biosynthesis in Bacillus cereus SPV under varied limiting conditions and an insight into the biosynthetic genes involved.

AIMS: A new strain of Bacillus, Bacillus cereus SPV, was found to be capable of using a wide range of carbon sources for the production of polyhydroxyalkanoates (PHAs) (Valappil et al. 2007b). Limiting nutrient in the culture conditions is crucial for PHA production. In this study, B. cereus SPV was grown in different culture conditions with limitation of potassium, nitrogen, sulphur and phosphorous to establish the impact of nutritional limitation on PHA production. METHODS AND RESULTS: The PHA yields obtained were found to be 13.4, 38, 13.15 and 33.33% dcw for potassium, nitrogen, sulphur and phosphorus limitations, respectively. Gas chromatography-mass spectrometry analysis of the isolated polymers showed the presence of P(3HB) under nitrogen, sulphur and phosphate-limiting conditions and P(3HB-3HV) copolymer under potassium limiting conditions. This ability of B. cereus SPV to accumulate different PHA monomers from structurally unrelated carbon sources led to an interest in the molecular analysis of PHA biosynthesis in this organism. To achieve this, PCR was used to identify the polyhydroxyalkanoate biosynthetic genes in B. cereus SPV. CONCLUSION: Sequence analysis of the PCR products from B. cereus SPV revealed the sequence of the putative biosynthetic genes, and possible regions involved in substrate binding. The nucleotide sequence reported in this paper is in the GenBank nucleotide sequence database under accession number DQ486135. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first report comparing the capability of B. cereus SPV to produce PHAs under different culture conditions of potassium, nitrogen, sulfur and phosphate limitations. The results in this study suggest the unique ability of B. cereus SPV to supply both 3HB and 3HV monomers from a structurally unrelated carbon source, glucose.

Installation of solarium--2000 for phototherapy.

Sunlight is the cheapest and most easily available source of ultraviolet light for phototherapy. The disadvantage of using sunlight as the source of ultraviolet light includes its variation with time , place and season, lack of privacy, and atmospheric conditions. To ensure correct dose of UV light in spite of variation in irradiation from the sunlight,we used a PMA2100 meter [Figure:2] and 2110 UVA detector ( Solar light Co, Philadelphia). Privacy for the patients was achieved by erecting a solarium and incidental UVB radiation was cut off by using window glass as the roof of the solarium. We recommend the solarium as a less expensive alternative to artificial phototherapy units, especially in places where there is plenty of sunlight.

Optimization of a screening lead for factor VIIa/TF.

The structure-based design and progression of a screening lead to a 3nM factor VIIa/TF inhibitor with improved selectivity versus related enzymes is described.

Evaluation of garlic oil as a contact dissolution agent for gallstones: comparison with monooctanoin.

AIM: To find a better contact solvent to dissolve gallstones we studied invitro use of garlic oil and compared it with monooctanoin. METHODS: In the first stage gall stones obtained from patients after cholecystectomy were subjected to dissolution in different concentrations of garlic oil. Then the rate of dissolution of gall stones in garlic oil was compared to that in monooctanoin in stage II. Efficacy of various concentration of garlic oil and monoocatanoin in dissolving gallstones in artificial gall bladder and common bile duct models were compared in stage III. RESULTS: Garlic oil dissolved the cholesterol gall stones in proportion to the concentration used. The gall stone fragmentation was faster [6 hours V/s 36 hours] and more [88.30% V/s 71.01%] by garlic oil in comparison to monooctanoin in test tubes and even in artificial gallbladder and common bile duct models. CONCLUSIONS: Garlic oil is a better contact dissolving agent of gallstones than monooctanoin.

Influence of noise spectra on auditory frequencies & degree of temporary threshold shift.

Influence of noise spectra on auditory frequencies of 131 audiometrically normal human volunteers was assessed. Each subject was exposed to four different types of noise on different days for a period of 30 min. Noises utilized in this study were broadband noise (100 dBA), broadband noise (BBN) mixed independently with tones of 0.5, 1.2 and 4 kHz at three intensity levels (85, 95 and 105 dBA) and noises of different bandwidths (100 dBA) around the above tones as centre frequencies. BBN produced maximum TTS2 at 4 kHz (21.42 +/- 0.61 to 26.15 +/- 1.00 dB), while concentration of sound energy around these tones, affected hearing at one-half octave above the point of concentration of sound energy. TTS2 produced by 4 kHz tonal component of 95 and 105 dBA and 2 kHz of 105 dBA were significantly higher than the one produced by BBN at 4 kHz. The single tones of 2 and 4 kHz and noise of 1/3rd octave bandwidth (cf 4 kHz) produced TTS2s which were significantly higher as compared to that of BBN at 4 kHz. At the same centre frequency, noise concentrated in thinner bands was more injurious than that in the thicker bands. Lower frequencies of hearing appears to be more resistant to noise as for the same sound pressure level the TTS2 observed in low frequencies was less as compared to higher frequencies.