RESUMEN
The aim of this study was to investigate the sedative activity of the aqueous leaf extract of Passiflora quadrangularis, a species that is widely cultivated and consumed in South America, and to identify its main constituents and elucidate the involvement of the GABAergic pathway in its mechanism of action. The bioguided fractionation of the crude extract showed a positive relationship between the sedative activity of the extract and its flavonoids. The methods employed to identify and isolate its main flavonoids resulted in the identification of vitexin-2''-O-xyloside, vitexin-2''-O-glucoside, orientin-2''-O-xyloside and orientin-2''-O-glucoside. Vitexin-2"-O-xyloside, the major flavonoid of the extract, showed sedative activity after oral administration in mice.
Asunto(s)
Flavonoides/farmacología , Neuronas GABAérgicas/fisiología , Hipnóticos y Sedantes/farmacología , Passiflora , Extractos Vegetales/farmacología , Ácido gamma-Aminobutírico/fisiología , Animales , Diazepam/farmacología , Relación Dosis-Respuesta a Droga , Flavonoides/aislamiento & purificación , Moduladores del GABA/farmacología , Neuronas GABAérgicas/efectos de los fármacos , Hipnóticos y Sedantes/aislamiento & purificación , Masculino , Ratones , Extractos Vegetales/aislamiento & purificación , Transducción de Señal/efectos de los fármacos , Transducción de Señal/fisiologíaRESUMEN
ABSTRACT Several species of the genus Passiflora are distributed all over South America, and many of these species are used in popular medicine, mainly as sedatives and tranquilizers. This study analyzes the chemical profile of extracts of four Passiflora species used in folk medicine, focusing on the flavonoids, alkaloids and saponins. We employed simple and fast fingerprint analysis methods by high performance liquid chromatography, ultra performance liquid chromatography and capillary electrophoresis techniques. The analysis led to the detection and identification of C-glycosylflavonoids in all the plant extracts, these being the main constituents in P. tripartita var. mollissima and P. bogotensis. Saponins were observed only in P. alata and P. quadrangularis, while harmane alkaloids were not detected in any of the analyzed extracts in concentrations higher than 0.0187 ppm, the detection limit determined for the UPLC method.
RESUMEN
In this study, we applied a gradient High-Speed Counter-Current Chromatography (HSCCC) method that allowed, by direct injection of an aqueous crude extract of the leaves of Passiflora bogotensis, the successful isolation of six flavonoids in a single run, with purity of each compound higher than 81%. This separation enabled the isolation of two new flavonoid glycosides, apigenin-6-C-α-l-rhamnopyranosyl-(1â2)-(6â³-O-acetyl)-ß-d-glucopyranoside (2) and luteolin-6-C-α-l-rhamnopyranosyl-(1â2)-(6â³-O-acetyl)-ß-d-glucopyranoside (4), and four known ones, isovitexin (1), isoorientin (3), isovitexin-2â³-O-rhamnoside (5) and isoorientin-2â³-O-rhamnoside (6). The structures of the isolated compounds were identified by HPLC-DAD, LC-MS, (1)H and (13)C NMR and comparison with literature data. The inhibitory activities of all of these compounds were evaluated in vitro on α-glucosidase from S. cerevisiae, and the IC50 was determinate. This is the first study concerning the chemical composition and biological activity of Passiflora bogotensis.