RESUMEN
The objective of this study was to test the hypothesis that Leptin induced in vitro growth in preantral follicles in sheep involves modulation of P450 aromatase expression and steroidogenesis. Accordingly, the expression of P450 aromatase gene was studied in the cumulus cells and oocytes isolated from different stages of preantral follicles (PFs') grown in vivo, cultured in TCM 199B, TCM 199B + Leptin (10 ng/ml) (TCM199BL) or a standard PF culture medium supplemented with Leptin (10 ng/ml) (SML). Ovarian follicles grown in vivo or in SML expressed P450 aromatase both in cumulus cells and oocytes at all the development stages. In the oocytes from PFs' grown in vitro, P450 expression was consistently lower than in those from in vivo grown follicles at all except the preantral stage. The patterns of expression of aromatase gene in the cumulus cells from in vivo grown and the PFs' cultured in TCM 199BL were similar. Significantly higher levels of progesterone production were supported by SML at all the development stages than the other two media. Oestradiol concentration in the spent TCM 199B and SML showed a significant increase as the development progressed from preantral to large antral stage. However, such increase was not sustained beyond early antral stage in the PFs' cultured in TCM199BL. It is concluded that Leptin modulates the expression P450 aromatase while supporting the in vitro development of the ovarian follicles in sheep.
Asunto(s)
Aromatasa/metabolismo , Estrógenos/biosíntesis , Leptina/farmacología , Folículo Ovárico/efectos de los fármacos , Progesterona/biosíntesis , Ovinos , Animales , Aromatasa/genética , Medios de Cultivo , Femenino , Regulación Enzimológica de la Expresión Génica/efectos de los fármacos , Técnicas de Cultivo de Tejidos/veterinariaRESUMEN
In rats with adjuvant-induced arthritis, the effect of (+)-catechin (CA) and 0-(beta-hydroxyethyl) rutosides (HR) on the crosslinking of collagen was studied. Compared with controls the arthritic group showed an increase in the reversibility of neutral salt-soluble collagen gel, solubility of acid-insoluble collagen to denaturing agents and the ratio of alpha/beta subunits of neutral salt-soluble collagen. These results suggest an impaired maturation of collagen in arthritic animals. Administration of CA or HR to the arthritic animals caused a decrease in the reversibility of collagen gel and in the solubility of acid-insoluble collagen to denaturing agents and to pronase. In addition, the electrophoretic patterns of neutral salt-soluble collagen on SDS polyacrylamide gels also showed a decrease in the alpha/beta ratio. All these results may collectively indicate that both flavonoids promote the crosslinking of collagen in arthritic animals.
Asunto(s)
Artritis Experimental/metabolismo , Artritis/metabolismo , Colágeno/metabolismo , Flavonoides/farmacología , Animales , Catequina/farmacología , Hidroxietilrutósido/farmacología , Técnicas In Vitro , Masculino , Ratas , Piel/metabolismoRESUMEN
In rats with adjuvant induced arthritis, the effect of CA or HR on the metabolism of collagen in skin was studied. In untreated animals with adjuvant induced arthritis, the synthesis of collagen is decreased, the catabolism of newly formed collagen is intensified and the conversion of soluble collagen to insoluble collagen is impaired. Bioflavonoids were found to increase the collagen synthesis, accelerate the conversion of soluble collagen to insoluble collagen and inhibit the catabolism of soluble collagen. The beneficial action of bioflavonoids on the metabolism of collagen in adjuvant induced arthritis was discussed.