Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 4 de 4
Filtrar
Más filtros

Bases de datos
Tipo del documento
País de afiliación
Intervalo de año de publicación
1.
Molecules ; 26(13)2021 Jun 25.
Artículo en Inglés | MEDLINE | ID: mdl-34202230

RESUMEN

Prediabetes (PrDM) is a prodromal stage of diabetes mellitus (DM) with an increasing prevalence worldwide. During DM progression, individuals gradually develop complications in various organs. However, lungs are suggested to be affected later than other organs, such as the eyes, heart or brain. In this work, we studied the effects of PrDM on male Wistar rats' lungs and whether the regular consumption of white tea (WTEA) for 2 months contributes to the improvement of the antioxidant profile of this tissue, namely through improved activity of the first line defense antioxidant enzymes, the total antioxidant capacity and the damages caused in proteins, lipids and histone H2A. Our data shows that PrDM induced a decrease in lung superoxide dismutase and glutathione peroxidase activities and histone H2A levels and an increase in protein nitration and lipid peroxidation. Remarkably, the regular WTEA intake improved lung antioxidant enzymes activity and total antioxidant capacity and re-established the values of protein nitration, lipid peroxidation and histone H2A. Overall, this is the first time that lung is reported as a major target for PrDM. Moreover, it is also the first report showing that WTEA possesses relevant chemical properties against PrDM-induced lung dysfunction.


Asunto(s)
Diabetes Mellitus Experimental/metabolismo , Pulmón/metabolismo , Estrés Oxidativo/efectos de los fármacos , Extractos Vegetales/farmacología , Estado Prediabético/metabolismo , Té/química , Animales , Antioxidantes/metabolismo , Biomarcadores/metabolismo , Glutatión Peroxidasa/metabolismo , Histonas/metabolismo , Peroxidación de Lípido/efectos de los fármacos , Masculino , Extractos Vegetales/química , Procesamiento Proteico-Postraduccional/efectos de los fármacos , Ratas , Ratas Wistar , Superóxido Dismutasa/metabolismo
2.
J Nutr Biochem ; 37: 83-93, 2016 11.
Artículo en Inglés | MEDLINE | ID: mdl-27637002

RESUMEN

Prediabetes has been associated with alterations in male reproductive tract, especially in testis and epididymis. Moreover, in vitro studies described a promising action of tea (Camellia sinensis L.) against metabolic dysfunctions. Herein, we hypothesized that white tea (WTEA) ingestion by prediabetic animals could ameliorate the metabolic alterations induced by the disease in testicular and epididymal tissues, preserving sperm quality. WTEA infusion was prepared and its phytochemical profile was evaluated by 1H-NMR. A streptozotocin-induced prediabetic rat model was developed and three experimental groups were defined: control, prediabetic (PreDM) and prediabetic drinking WTEA (PreDM+WTEA). Metabolic profiles of testis and epididymis were evaluated by determining the metabolites content (1H-NMR), protein levels (western blot) and enzymatic activities of key metabolic intervenient. The quality of spermatozoa from cauda epididymis was also assessed. Prediabetes increased glucose transporter 3 protein levels and decreased lactate dehydrogenase activity in testis, resulting in a lower lactate content. WTEA ingestion led to a metabolic adaptation to restore testicular lactate content. Concerning epididymis, prediabetes decreased the protein levels of several metabolic intervenient, resulting in decreased lactate and alanine content. WTEA consumption restored most of the evidenced alterations, however, not lactate content. WTEA also improved epididymal sperm motility and restored sperm viability. Prediabetes strongly affected testicular and epididymal metabolic status and most of these alterations were restored by WTEA consumption, resulting in the improvement of sperm quality. Our results suggest that WTEA consumption can be a cost-effective strategy to improve prediabetes-induced reproductive dysfunction.


Asunto(s)
Epidídimo/metabolismo , Conservación de Alimentos , Alimentos Funcionales , Infertilidad Masculina/prevención & control , Estado Prediabético/dietoterapia , , Testículo/metabolismo , Alanina/metabolismo , Animales , Supervivencia Celular , Epidídimo/enzimología , Epidídimo/patología , Alimentos Funcionales/análisis , Transportador de Glucosa de Tipo 3/metabolismo , Infertilidad Masculina/etiología , Infertilidad Masculina/metabolismo , Ácido Láctico/metabolismo , Masculino , Resonancia Magnética Nuclear Biomolecular , Estado Prediabético/inducido químicamente , Estado Prediabético/metabolismo , Estado Prediabético/fisiopatología , Distribución Aleatoria , Ratas Wistar , Motilidad Espermática , Espermatogénesis , Espermatozoides/metabolismo , Espermatozoides/patología , Estreptozocina/toxicidad , Té/química , Testículo/enzimología , Testículo/patología
3.
Reprod Biomed Online ; 31(4): 544-56, 2015 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-26276042

RESUMEN

Prediabetes represents a major risk factor for the development of type 2 diabetes mellitus (T2DM). It encompasses some, but not all, T2DM diagnostic criteria. Prediabetes has been recently associated with altered testicular function and increased testicular oxidative stress (OS). Tea is widely consumed and its anti-hyperglycaemic/antioxidant properties are known. This study aimed to evaluate whether white tea (WTEA) consumption by prediabetic rats could prevent testicular OS, preserving sperm quality. For that purpose, WTEA (presenting a high catechin content) was given to 30-day-old streptozotocin-induced prediabetic rats for 2 months. Testicular antioxidant potential and OS were evaluated, as well as sperm parameters, by standard techniques. WTEA consumption improved glucose tolerance and insulin sensitivity in prediabetic rats. Testicular antioxidant potential was increased by WTEA consumption, restoring protein oxidation and lipid peroxidation, although glutathione content and redox state were not altered. WTEA consumption improved sperm concentration and sperm quality (motility, viability and abnormality) was restored. Overall, WTEA consumption improved reproductive health of male prediabetic rats. Based on the study results, WTEA consumption appears to be a natural, economical and effective strategy to counteract the deleterious effects of prediabetes on male reproductive health, but further studies will be needed before a definitive recommendation is made.


Asunto(s)
Estrés Oxidativo , Estado Prediabético/dietoterapia , Análisis de Semen , , Testículo/metabolismo , Animales , Complicaciones de la Diabetes/dietoterapia , Complicaciones de la Diabetes/etiología , Complicaciones de la Diabetes/patología , Diabetes Mellitus Experimental/dietoterapia , Diabetes Mellitus Experimental/patología , Diabetes Mellitus Experimental/fisiopatología , Prueba de Tolerancia a la Glucosa , Glutatión/metabolismo , Infertilidad Masculina/dietoterapia , Infertilidad Masculina/etiología , Infertilidad Masculina/patología , Resistencia a la Insulina , Peroxidación de Lípido , Masculino , Fitoquímicos/química , Estado Prediabético/patología , Estado Prediabético/fisiopatología , Carbonilación Proteica , Ratas , Ratas Wistar , Recuento de Espermatozoides , Motilidad Espermática , Espermatozoides/anomalías , Té/química
4.
Mol Hum Reprod ; 20(11): 1067-76, 2014 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-25205674

RESUMEN

Melatonin co-operates with insulin in the regulation of glucose homeostasis. Within the testis, glucose metabolism in the somatic Sertoli cells (SCs) is pivotal for spermatogenesis. Since the effects of melatonin on male reproductive physiology remain largely unknown, we hypothesized that melatonin may affect spermatogenesis by modulating SC metabolism, interacting with insulin. To test our hypothesis, rat SCs were maintained in culture for 24 h in the presence of insulin, melatonin or both and metabolite production/consumption was determined by proton nuclear magnetic resonance ((1)H-NMR). Protein levels of glucose transporters (GLUT1 and GLUT3), phosphofructokinase 1, lactate dehydrogenase (LDH) and monocarboxylate transporter 4 were determined by western blot. LDH activity was also assessed. SCs treated with melatonin showed an increase in glucose consumption via modulation of GLUT1 levels, but decreased LDH protein expression and activity, which resulted in lower lactate production. Moreover, SCs exposed to melatonin produced and accumulated less acetate than insulin-exposed cells. The combined treatment (insulin plus melatonin) increased acetate production by SCs, but intracellular acetate content remained lower than in insulin exposed cells. Finally, the intracellular redox state, as reflected by intracellular lactate/alanine ratio, was maintained at control levels in SCs by melatonin exposure (i.e. melatonin, alone or with insulin, increased the lactate/alanine ratio versus cells treated with insulin). Furthermore, SCs exposed to insulin plus melatonin produced more lactate and maintained the protein levels of some glycolysis-related enzymes and transporters at control levels. These findings illustrate that melatonin regulates SCs metabolism, and thus may affect spermatogenesis. Since lactate produced by SCs provides nutritional support and has an anti-apoptotic effect in developing germ cells, melatonin supplementation may be an effective therapy for diabetic male individuals facing subfertility/infertility.


Asunto(s)
Glucólisis/efectos de los fármacos , Melatonina/farmacología , Células de Sertoli/efectos de los fármacos , Animales , Glucosa/metabolismo , Transportador de Glucosa de Tipo 1/metabolismo , Transportador de Glucosa de Tipo 3/metabolismo , Infertilidad Masculina/metabolismo , Insulina/farmacología , L-Lactato Deshidrogenasa/metabolismo , Masculino , Resonancia Magnética Nuclear Biomolecular , Oxidación-Reducción , Fosfofructoquinasa-1/metabolismo , Ratas , Ratas Wistar , Células de Sertoli/metabolismo
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA