[The gastroprotective action of acidic mineral water from the Azhyg-Sug source on the stress-induced injuries to the stomach of white rats]. / Gastroprotektivnoe deistvie kisloi mineral'noi vody istochnika Azhyg-Sug pri stress-indutsirovannom povrezhdenii zheludka u belykh krys.

BACKGROUND: The relevance of this study is determined by the need for the scientifically sound substantiation of the possibility for the use of acidic mineral waters in the treatment of digestive diseases taking into consideration their widespread application in ethnomedicine, in particular in the Republic of Tuva. AIM: The objective of the present study was to elucidate the gastroprotective action of acidic mineral water from the Azhyg-Sug source (Republic of Tyva) on the experimental animals as exemplified by the model of ulcerogenesis under the conditions of immobilization stress. MATERIAL AND METHODS: The experimental studies were carried out with the use of 32 white rats of the Wistar line. Ulcerogenesis was initiated by means of immobilization stress. We evaluated the pathomorphological characteristics of the gastric mucosa and the number of destructions based on the Pauls index. The state of the lipid peroxidation system and antioxidative protection were determined from the content of malon dialdehyde and reduced glutathione, extracellular catalase and superoxide dismutase activities in erythrocytes. RESULTS: The study has demonstrated that the mineral water from the Azhyg-Sug source slows down the development of the inflammatory and destructive necrotic processes in the mucosa of the stomach of albino rats. The depth of erosion in the animals receiving mineral water was 2.3 and 3.4 times lower than in the control animals (p≤0.05). The antioxidant effect of mineral water was confirmed by the 14-20% decrease of the MDA concentration as well as by the increase of the catalase and superoxide dismutase (SOD) activities by 21-25% and 20-30% respectively in comparison with the control animals. CONCLUSION: The Azhyg-Sug mineral water has the strong gastroprotective influence on the experimental animals having the induced neurogenic ulcer. One of the mechanisms underlying the gastroprotective action of the investigated mineral water arises from its ability to inhibit the processes of lipid peroxidation with the simultaneous enhancement of the activity of the antioxidant system of the organism.

[Antimutagenic and antioxidant features of confectionery products containing the powder from the leaves of Hippophae rhamnoides L.]

The aim of the work was to determine the possibility of using the powder from the leaves of Hippophae rhamnoides L. for enriching flour confectionery and to evaluate the antimutagenic and antioxidant activity of the product. The experiment was carried out on 24 white Wistar rats with initial body weight (b.w.) 180-200 g. The animals of the experimental group (n=8) received confection containing sea buckthorn powder at a rate of 20 mg per 100 g b.w. for 14 days on the background of a standard vivarium diet. The animals of the control and intact groups received confection containing no bioactive supplement at the same dose. Antimutagenic and antioxidant effects were estimated in a day after a single injection of cyclophosphamide at a dose of 20 mg/kg b.w. The number of chromosomal aberrations in bone marrow cells of white rats was counted and the activity of catalase, superoxide dismutase (SOD), the level of reduced glutathione and the concentration of TBA-active products in blood were evaluated. The intake of the confectionery containing the powdered H. rhamnoides leaves resulted in the 45% decrease of the number of damaged cells, 50% decrease of the proportion of cells with multiple chromosome breaks and 52% decrease of the number of achromatic gaps as compared to animals of the control group (n=8). The cake intake increased the activity of catalase (by 52%) and SOD (by 33%) and glutathione content (by 26%) in blood.

PHYTOTHERAPY OF ANXIETY DISORDERS / Монголын эм зүй, эм судлал

The experiments on white Wistar rats have shown that Cimicifuga dahurica (TURCZ) Maxim. tincture and Schizonepeta multifi da (L.)Briq. extract have anxiolytic effect. Cimicifuga dahurica tincture stimulates exploratory activity of animals in the open fi eld test increasing the number of rearings and entries in the central area of the open fi eld and decreasing the number of boles and grooming acts. The Schizonepeta multifi da dry extract in the range of 50 to 300 mg/kg increases the number of water intake in the Vogel confl ict test. The effi cacy of the tested remedies – Cimicifuga dahurica tincture (at the doses of 0.5 and 1.0 ml/kg) and Schizonepeta multifi da extract (in the range of 100-300 mg/ kg) was higher on some parameters than that of valerian preparations. Key words: anxiolytic effect, Cimicifuga dahurica (TURCZ) Maxim. tincture, Schizonepetam multifi da (L.) Briq. extract. Introduction The development of medicinal remedies for the correction of functional impairments of the nervous system is important due to the prevalence of neuro-psychic diseases. At the same time, widely used anxiolytics negatively affect the human body causing hyper-sedation, myorelaxation, etc. [1, 3, 8]. Therefore, the plant remedies which have few side effects are prospective for pharmacological correction of the nervous system disturbances as alternative therapy or auxiliary remedy for increasing the effi cacy of receptor preparations [2, 5]. In this context the perennial plants Cimicifuga dahurica (TURCZ) Maxim. and Schizonepeta multifi da (L.) Briq. used in folk medicine as sedatives and for headaches are of great interest in the therapy of anxiety disorders [6, 7]. The aim of the study was to evaluate the infl uence of Cimicifuga dahurica tincture and Schizonepeta multifi da dry extract on the behavior of white rats in test systems characterizing anxiolytic effect of the medicinal remedy.

[Effect of Hypecoum erectum extract on morphofunctional state of the liver in rats with tetracycline-associated hepatitis].

The effect of Hypecoum erectum L. extract on the morphofunctional condition of the liver in rats with experimental tetracycline-associated hepatitis was studied. The experiment included 40 albino rats Wistar. The animals treated with tetracycline hydrochloride (1.0 g/kg body weight) were exposed to the extract in a dose of 50 mg/kg for 5 days. On the 7th day of the experiment the following indices were determined: malonic dialdehyde concentration, catalase activity, the levels of ATP, pyruvate and lactate in the liver homogenate, as well as the blood levels of reduced glutathione. The liver pathomorphological investigation was applied. The H. erectum extract was shown to inhibit lipid peroxidation, to increase the activity of the host endogenous antioxidant system, to normalize the hepatocyte energy provision and to limit the liver degeneration.