RESUMEN
The formation of new scaffolds to enhance healing magnitude is necessarily required in biomedical applications. Granulation tissue formation is a crucial stage of wound healing in which granulation tissue grows on the surface of a wound by the formation of connective tissue and blood vessels. In the present study, porous hydrogels were synthesized using chitosan incorporating latex of the Calotropis procera plant by using a freeze-thaw cycle to stimulate the formation of granulation tissue and angiogenesis in wound healing applications. Structural analysis through Fourier transform infrared (FTIR) spectroscopy confirmed the interaction between chitosan and Calotropis procera. Latex extract containing hydrogel showed slightly higher absorption than the control during water absorption analysis. Thermogravimetric analysis showed high thermal stability of the 60:40 combination of chitosan (CS) and Calotropis procera as compared to all other treatments and controls. A fabricated scaffold application on a chick chorioallantoic membrane (CAM) showed that all hydrogels containing latex extract resulted in a significant formation of blood vessels and regeneration of cells. Overall, the formation of connective tissues and blood capillaries and healing magnitude decreased in ascending order of concentration of extract.
Asunto(s)
Calotropis/metabolismo , Quitosano/química , Hidrogeles/química , Neovascularización Fisiológica , Cicatrización de Heridas , Animales , Materiales Biocompatibles , Embrión de Pollo , Membrana Corioalantoides/metabolismo , Congelación , Látex/química , Ensayo de Materiales , Microscopía Electrónica de Rastreo , Extractos Vegetales/química , Polímeros/química , Regeneración , Espectroscopía Infrarroja por Transformada de Fourier , TermogravimetríaRESUMEN
We demonstrated one-step synthesis of silver nanoparticles (AgNPs) from Cassia fistula fruit extract and their antibacterial activity against E. coli and K. pneumoniae. Biogenic AgNPs were characterized by scanning electron microscopy, X-Ray diffraction and fourier transform infrared spectroscopy. Results confirmed spherical shaped AgNPs with an average crystallite size of â¼69 nm. Dose-dependent (0, 10, 20, 40 and 80 µg mL-1) growth kinetic studies showed 100% potency against E. coli (20 µg mL-1) and K. pneumoniae (80 µg mL-1) after 1 and 5 h, respectively. Surface morphology analysis revealed formation of groove/pits in the lysed cell membrane that eventually led to bacterial death.