Cryptanshinone extract of Salvia miltiorrhiza stimulates pediatric acute myeloid leukemia stem cell apoptosis and the anti-inflammatory mechanism via accelerating microRNA-211-5p to supress Janus kinase 2/signal transducer and activator of transcription 3 signaling pathway activation.

This study was designed to explore cryptanshinone (CPT) extract of Salvia miltiorrhiza stimulating pediatric acute myeloid leukemia (AML) stem cell (LSC) apoptosis and anti-inflammatory mechanism via accelerating microRNA (miR)-211-5p to restrain Janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3) pathway activation. Obtaining blood samples from pediatric acute myeloid leukemia patients and healthy volunteers and detecting miR-211-5p and JAK2 were performed. Purchase of the human AML cell line KG1a was conducted, and sorting of KG1a cells was to gain LSC. Test of miR-211-5p and JAK2, the phosphorylation of JAK2/STAT3 was implemented. Pretreatment of LSCs was with CPT. Variation of miR-211-5p and JAK2 in LSCs was via plasmid transfection to explore their actions in cell advancement with apoptosis and inflammation. Identification of the targeting of miR-211-5p with JAK2 was implemented. In results: MiR-211-5p was declined in endometrial cancer, while JAK2 was elevated; CPT was available to boost LSC apoptosis and restrain the inflammation; elevated miR-211-5p or repressive JAK2 was available to strengthen the acceleration of CPT on LSCs apoptosis and the repression of inflammation; MiR-211-5p targeted JAK2; augmented JAK2 was available to turn around the action of elevated miR-211-5p. We conclude that CPT extract of Salvia miltiorrhiza stimulated pediatric LSC apoptosis and restrained the inflammation via accelerating microRNA (miR)-211-5p to suppress JAK2/STAT3 pathway activation.

[Evaluation of the antiatherosclerotic properties of natural compounds of plant origin on cell cultures of the human aortic intima]. / Otsenka antiateroskleroticheskikh svoistv nekotorykh prirodnykh soedinenii rastitel'nogo proiskhozhdeniia na kul'ture kletok intimy aorty cheloveka.

Administration of alizole, berberine, pheonol and pheoniphlorine in doses of 30-100 micrograms/ml in the culture of the atherosclerotic human aortic intimal cells decreased the content of intracellular cholesterol by 35-41% and reduced the proliferative activity of the cells. In healthy donors administration of single doses of berberine and pheoniphlorine (0.9 g) increased the antiatherogenic potential of the serum which was estimated on the primary culture of the intimal cells.

[Antiatherogenic properties of the soy protein isolate 500 E]. / Antiaterogennye svoistva belkovogo izoliata soi 500 E.

Two and 4 hours after a single intake of 50 g of protein isolate soy 500 E in the food, the activity of lysosomal hydrolases in blood platelets, the cholesterol (CS) content in lipoprotein-containing immune complexes, and the accumulation of CS in the subendothelial cells of human aorta intima reduced in introduction into a culture of serum obtained after intake of protein isolate soy. Direct introduction of a isolate 500 E water-alcohol extract into a culture of atherosclerotic plaques of human aorta intima caused decrease of the CS content in the cells and their proliferation. Protein isolate soy, therefore, produces an antiatherogenic effect in a cell culture and causes the antiatherosclerotic properties of serum.